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Differential Acridine Orange Staining of Human Chromosomes

Published online by Cambridge University Press:  27 January 2025

G.L. Castoldi ,
G.D. Grusovin ,
G.L. Scapoli  and
R. Spanedda
G.L. Castoldi*
Affiliation:
Medical Clinic of the University, Ferrara, Italy
G.D. Grusovin
Affiliation:
Medical Clinic of the University, Ferrara, Italy
G.L. Scapoli
Affiliation:
Medical Clinic of the University, Ferrara, Italy
R. Spanedda
Affiliation:
Medical Clinic of the University, Ferrara, Italy
*
Clinica Medica dell'Università, Ferrara, Italy

Summary

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The acridine orange staining of metaphases previously treated with hot salt solutions, exhibits a differential banding pattern of the chromosomes. According to the physicochemical interpretation of the stained structures, the green and red fluorescent segments of the chromosomes should be considered as constituted respectively by double-stranded DNA and single-stranded DNA. The banding pattern is relatively consistent in different metaphases, although some occasional variations of the bands may be referred to the interference of chromosomal acid proteins. In general, the sequence of the bands is compatible with the picture of the reverse banding.

Riassunto

Riassunto

L'applicazione della colorazione con arancio di acridina a metafasi precedentemente trattate con soluzioni saline a caldo fornisce, in fluorescenza, una colorazione differenziale di bande a livello dei cromosomi. L'interpretazione fisico-chimica delle modalità di estrinsecazione della colorazione consente di definire come composte da DNA double stranded le regioni colorate in verde e come composte da DNA single stranded quelle colorate metacromaticamente in rosso arancio. La dislocazione delle bande appare relativamente costante, seppure con occasionali deformazioni, per la possibile interferenza di strutture quali mucopolisaccaridi e proteine acide, nelle fasi di colorazione. Il quadro presentato dalla sequenza delle bande lungo i singoli cromosomi sembra nel complesso sovrapporsi a quello del reverse banding.

Résumé

Résumé

La coloration de métaphases préalablement traitées par des solutions salines à chaud, avec orange d'acridine, démontre la présence de bandes différemment fluorescentes à niveau des chromosomes. L'interprétation des modalités physico-chimiques de la coloration permet de considérer comme constituées par double-stranded DNA les régions chromosomiques qui donnent une fluorescence verte et par single-stranded DNA les régions métachromatiquement colorées en rouge-orange. La disposition des bandes est relativement constante dans des différentes métaphases, bien que des variations occasionnelles peuvent se manifester peut-être par rapport à l'interférence de protéines acides le long des chromosomes. Le tableau présenté par les bandes fluorescentes au niveau des chromosomes est dans l'ensemble comparable à celui du reverse banding.

Zusammenfassung

Zusammenfassung

Die durch AO Färbung von mit Salzlösungen im voraus behandelten Metaphasen zeigt eine Reihe unterscheidlich fluoreszierender Streifen der Chromosomen. Die physico-chemische Grundlage der Färbung mit AO erlauben die grüne fluoreszierende Streifen der Chromosomen als von double-stranded DNA und die röte fluoreszierende Streifen als von single-stranded DNA zusammengesetzt zu betrachten. Die Streifenanordnung scheint relativ konstant zu sein; einige Abweichungen sind in der Hauptsache zu der Interferenz säurer proteischen Strukturen zurückzuführen. Im allegemeinen ist die Streifenreihe vergleichbar mit dem Bild des reverse banding.

Type
Research Article
Information
Acta geneticae medicae et gemellologiae: twin research , Volume 21 , Issue 4 , October 1972 , pp. 319 - 326
Copyright
Copyright © The International Society for Twin Studies 1978

References

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