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Blood protein polymorphism and genetic diversity in locally adapted Muscovy duck (Cairina moschata) in Nigeria

Published online by Cambridge University Press:  28 January 2015

A.O. Oguntunji  and
K.L. Ayorinde
A.O. Oguntunji*
Affiliation:
Department of Animal Science and Fisheries Management, Bowen University, Iwo, Osun State, Nigeria
K.L. Ayorinde
Affiliation:
Department of Animal Production, University of Ilorin, Ilorin, Kwara State, Nigeria
*
Correspondence to: A.O. Oguntunji, Department of Animal Science and Fisheries Management, Bowen University, Iwo, Osun State, Nigeria. email: abelmendel@yahoo.co.in
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Summary

Characterization and genetic diversities among members of a species are fundamental to their improvement and conservation. This study was conducted to characterize and estimate genetic diversity in the ecotypes of the locally adapted Muscovy duck in Nigeria using blood proteins (haemoglobin, transferrin and albumin) and enzyme (carbonic anhydrase) markers. Blood samples collected from 20, 40 and 20 adult Muscovy ducks sampled randomly from the Rain Forest, Derived Savanna and Guinea Savanna ecotypes, respectively, were typed for blood proteins and enzyme polymorphism with cellulose acetate paper. Genetic variability in the studied population was accessed using heterozygosity (H), effective number of allele (ne) and polymorphism (percent P). All the 13 allelic variants expressed at the four loci were expressed in the Derived Savanna ecotype but 12 each in the Rain Forest and Guinea Savanna ecotypes. Results showed that all the four loci were polymorphic (100 percent) and the estimated heterozygosity among them was similar (0.424–0.481). Similarity in the estimated genetic variability parameters among ecotypes indicate that the sub-populations understudy was under similar evolutionary forces and there were no appreciable differences among them.

Résumé

La caractérisation et les diversités génétiques parmi les members d'une spécie sont fondamentales pour leurs ameliorations afin de les conservation. Cet etude a ete sait pour pourvoir caracterises et estimer la diversite genetique dans les ecotypes des carnards locaux muscovys au Nigeria en utilisant le sang protein(Haemoglobine, le transferine et l'albumin) et l ’ enzyme (L'anhydrase carbonique) marquers. Les echantillions saguines ramasses de 20, 40 et 20 adultes des carnards Muscovy ont ete pris respectivement par hazard des ecotypes de la foret, de la savvane derive, et de la savanne guinea. Ils ont ete tapes pour les proteins sanguine et l’ enzymes polymorphism eaves les papierrs cellulose. La variable genetique de la population etudie a ete evalue en utilisant heterozygosite (H) les nombres des alleles (ne) effectifs et la polymorphisme (%P0. Tous les treize variants alleliques montres dans les quatre locis sout aussi montres dans l'ecotype de la savanne derive mais les douze dans la foret et daus les ecotype de la guinea savanne. Les resultants montret que tous les quatre locis sont polymorphiques (100%0 et l'heterozygosite estimee parmi eux etait semblable (0.424–0.481). La similarite de ĺa parameter variable de la genetique estmee parmie les ecotypes indique que les sousopopulations etudiees etaient similaires aux forces evolutionaire et il n’ y avait pas de beaucoup difference parmi eux.

Resumen

La caracterización y las diversidades genéticas entre miembros de una especie son fundamentales para su mejora y conservación. Este estudio fue llevado a cabo con el fin de caracterizar y estimar la diversidad genética en los ecotipos del pato mudo localmente adaptado en Nigeria, usando proteínas sanguíneas (hemoglobina, transferrina y albúmina) y marcadores enzimáticos (anhidrasa carbónica). Las muestras de sangre se tomaron en 20, 40 y 20 patos mudos adultos recogidos al azar de los ecotipos Selva, Sabana Derivada y Sabana de Guinea, respectivamente. En las muestras de sangre, se determinaron las proteínas sanguíneas y el polimorfismo enzimático con papel de acetato de celulosa. La variabilidad genética de la población estudiada fue evaluada en base a la heterocigosis (H), el número efectivo de alelos (ne) y el polimorfismo (P, en tanto por ciento). Las trece variantes alélicas asociadas a los cuatro loci se expresaron en el ecotipo Sabana Derivada mientras que en los ecotipos Selva y Sabana de Guinea sólo se expresaron doce. Los resultados mostraron que los cuatro loci fueron polimórficos (100 por cien) y que la heterocigosis estimada fue similar entre ellos (0.424–0.481). La similitud entre ecotipos en las medidas de estimación de la variabilidad genética indica que las subpoblaciones objeto de estudio se hallaban bajo similares fuerzas evolutivas y que no existían diferencias apreciables entre ellas.

Keywords

blood proteincharacterizationgenetic diversityheterozygosityMuscovy ducksLa polymorphisme de la protéin sanguine et la g énétique diversit é des carnards locaux moscovy (Cairina moschata) au NigeriaProtéin sanguinecaracterisationla diversite genetiquel’ heterozysitele carnard muscovyproteína sanguíneacaracterizacióndiversidad genéticaheterocigosispatos mudos
Type
Research Article
Information
Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales , Volume 56 , June 2015 , pp. 9 - 18
Copyright
Copyright © Food and Agriculture Organization of the United Nations 2015 

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