Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-10T15:57:33.376Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluación del semen criopreservado de toros Curraleiro Pé Duro, perteneciente al Banco Brasilero de Germoplasma Animal

Published online by Cambridge University Press:  01 May 2014

I.S. Montesinos ,
J.O. Carvalho ,
J.V. Malaquias ,
E. Arnhold ,
G.E. Freneau ,
M.A.N. Dode ,
M.C.S. Fioravanti  and
J.R.B. Sereno
I.S. Montesinos*
Affiliation:
Universidad Federal de Goiás, CEP 74001-970. Goiânia-GO, Brasil
J.O. Carvalho
Affiliation:
Embrapa Cenargen, CEP 70770-900, Brasilia-DF, Brasil
J.V. Malaquias
Affiliation:
Embrapa Cerrados, CEP 73310-970, Planaltina-DF, Brasil
E. Arnhold
Affiliation:
Universidad Federal de Goiás, CEP 74001-970. Goiânia-GO, Brasil
G.E. Freneau
Affiliation:
Universidad Federal de Goiás, CEP 74001-970. Goiânia-GO, Brasil
M.A.N. Dode
Affiliation:
Embrapa Cenargen, CEP 70770-900, Brasilia-DF, Brasil
M.C.S. Fioravanti
Affiliation:
Universidad Federal de Goiás, CEP 74001-970. Goiânia-GO, Brasil
J.R.B. Sereno
Affiliation:
Embrapa Cerrados, CEP 73310-970, Planaltina-DF, Brasil
*
Correspondencia: I.S. Montesinos, Universidad Federal de Goiás, Brasil. e-mail: ivansm.vet@gmail.com
Get access

Summary

This investigation aimed to evaluate the quality of cryopreserved semen from 21 Curraleiro Pé Duro bulls in a germplasm bank, categorizing the doses of the sires according to parameters related to sperm viability, and verifying the fertilizing capacity in vitro of the spermatozoids. All the samples were thawed and analyzed as to morphology, kinetics, plasma membrane integrity and acrosome integrity. Samples of only four bulls were used for evaluating the fertilization rates. The results indicated that the Curraleiro Pé Duro semen doses, stocked in the Brazilian Animal Germplasm Bank, showed variable sperm quality and high in vitro fertilizing capacity, making it possible to design appropriate strategies for assisted reproduction in order to conserve this Brazilian genetic resource, which is in danger of extinction.

Resumen

Esta investigación tuvo el objetivo de evaluar la calidad del semen criopreservado de 21 toros Curraleiro Pé Duro en banco de germoplasma, categorizando las dosis de los reproductores según parámetros relacionados a la viabilidad espermática, y verificando la capacidad fecundante in vitro de los espermatozoides. Todas las muestras fueron descongeladas y analizadas en cuanto a morfología, cinética, integridad de membrana plasmática e integridad de acrosoma. Para evaluar las tasas de fecundación, muestras de sólo cuatro toros fueron utilizadas. Los resultados indicaron que las dosis de semen Curraleiro Pé Duro, almacenadas en el Banco Brasilero de Germoplasma Animal, mostraron calidad espermática variable y alta capacidad fecundante in vitro, posibilitando delinear estrategias adecuadas de reproducción asistida para la conservación de este recurso genético brasilero en peligro de extinción.

Résumé

Cette recherche visait à évaluer la qualité du sperme cryoconservés de 21 taureaux Curraleiro Pé Duro dans une banques de gènes, categorisant les doses des reproduteurs selon les paramètres liés à la viabilité du sperme, et de vérifier la capacité fecondante in vitro des spermatozoïdes. Tous les échantillons ont été décongelés et analysés pour la morphologie, la cinétique et la membrane plasmique et intégrité de l'acrosome. Pour évaluer le taux de fecondation, des échantillons de seulement quatre taureaux ont été utilisés. Les résultats ont indiqués que les doses de sperme Curraleiro Pé Duro, stokée dans la Banque Bresilienne de Gènes Animal, ont montré de haute qualité du sperme variable capacité de fécondation in vitro, permettant délimiter les stratégies appropriés de reproduction assistée pour la préservation de cette ressource génétique bresilienne en voie de disparition.

Keywords

conservación ex situespermatozoiderazas criollascreole breedsex situ conservationspermatozoidcréoles racesla conservation ex situspermatozoïde
Type
Research Article
Information
Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales , Volume 54 , June 2014 , pp. 135 - 140
Copyright
Copyright © Food and Agriculture Organization of the United Nations 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Referencias

Amann, R.P. 1989. Can the fertility potential of a seminal sample be predicted accurately? Journal of Andrology 10 (2): 89–98.Google Scholar
Barbas, J.P. & Mascarenhas, R.D. 2009. Cryopreservation of domestic animal sperm cells. Cell and Tissue Banking 10: 49–62.Google Scholar
Barth, A.D. & Oko, R.J. 1989. Abnormal morphology of bovine spermatozoa. Iowa State University Press, Ames (IA), USA.Google Scholar
Britto, C.M.C. & Mello, M.L.S. 1988. Anomalias de cabeça em espermatozóides de gado “Pé Duro”. Revista Brasileira de Genética 11 (1): 63–71.Google Scholar
Carvalho, J.O., Sartori, R., Machado, G.M., Mourão, G.B. & Dode, M.A.N. 2010. Quality assessment of bovine cryopreserved sperm after sexing by flow cytometry and their use in in vitro embryo production. Theriogenology 74: 1521–1530.Google Scholar
CBRA. 1998. Manual para exame andrológico e avaliação de sêmen animal. 2da edición, Colégio Brasileiro de Reprodução Animal, Belo Horizonte, Brasil.Google Scholar
Dode, M.A.N. & Rumpf, R. 2002. Produção in vitro de embriões na espécie bovina. Biotecnologia Ciência & Desenvolvimento 26: 32–37.Google Scholar
Eid, L.N., Lorton, S.P. & Parrish, J.J. 1994. Paternal influence on S-Phase in the first cell cycle of the bovine embryo. Biology of Reproduction 51: 1232–1237.Google Scholar
Fioravanti, M.C.S., Juliano, R.S., Costa, G.L., Abud, L.J., Cardoso, V.S., Carpio, M.G. & Costa, M.F.O. 2011. Conservación del bovino Curraleiro: cuantificación del censo y caracterización de los criadores. Animal Genetic Resources 48: 109–116.Google Scholar
Freneau, G.E. 2011. Aspectos da morfologia espermática em touros. Revista Brasileira de Reprodução Animal 35 (2): 160–170.Google Scholar
Garner, D.L., Pinkel, D., Johnson, L.A. & Pace, M.M. 1986. Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analyses. Biology of Reproduction 34: 127–138.Google Scholar
Graham, J.K. 2001. Assessment of sperm quality. En: Proceedings of the 47th Annual Convention of the AAEP, 24–28 November 2001, San Diego (CA), USA.Google Scholar
Harrison, R.A.P. & Vickers, S.E. 1990. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. Journal of Reproduction and Fertility 88: 343–352.Google Scholar
Januskauskas, A., Gil, J., Soderquist, L., Haard, M.G.M., Haard, M.C., Johannisson, A. & Rodriguez-Martinez, H. 1999. Effect of cooling rates on post-thaw sperm motility, membrane integrity, capacitation status and fertility of dairy bull semen used for artificial insemination in Sweden. Theriogenology 52: 641–658.Google Scholar
Januskauskas, A., Johannisson, A. & Rodriguez-Martinez, H. 2003. Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility. Theriogenology 60: 743–758.Google Scholar
Kathiravan, P., Kalatharan, J., Karthikeya, G., Rengarajan, K. & Kadirvel, G. 2011. Objective sperm motion analysis to asses dairy bull fertility using computer-aided system – a review. Reproduction in Domestic Animals 46: 165–172.Google Scholar
Klinc, P. & Rath, D. 2007. Reduction of oxidative stress in bovine spermatozoa during flow cytometric sorting. Reproduction in Domestic Animals 42: 63–67.Google Scholar
Mariante, A.S. & Egito, A.A. 2002. Animal genetic resources in Brazil: Result of five centuries of natural selection. Theriogenology 57: 223–235.CrossRefGoogle ScholarPubMed
Mariante, A.S., Albuquerque, M.S.M., Egito, A.A., McManus, C., Lopes, M.A. & Paiva, S.R. 2009. Present status of the conservation of livestock genetic resources in Brazil. Livestock Science 120: 204–212.Google Scholar
Mortimer, S.T. 1997. A critical review of the physiological importance and analysis of sperm movement in mammals. Human Reproduction Update 3 (5): 403–439.Google Scholar
Obando, H., Luque, O. & Martínez, R. 2003. Evaluación de la capacidad fertilizante in vitro del semen de toros San Martinero y Brahman. ICA Informa 30 (2): 56–62.Google Scholar
R Development Core Team. 2011. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Disponible en http://www.r-project.org/.Google Scholar
Rao, A.R., Bane, A. & Gustafsson, B.K. 1980. Changes in the morphology of spermatozoa during their passage through the genital tract in dairy bulls with normal and impaired spermatogenesis. Theriogenology 14: 1–12.Google Scholar
Rodriguez-Martinez, H. 2005. Methods for semen evaluation and their relationship to fertility. En: Anais do 16° Congresso Brasileiro de Reprodução Animal, 1–5 Agosto 2005, Goiânia (GO), Brasil.Google Scholar
Saacke, R.G. 2008. Sperm morphology: Its relevance to compensable and uncompensable traits in semen. Theriogenology 70: 473–478.Google Scholar
Sartorio, S.D. 2008. Aplicações de técnicas de análise multivariada em experimentos agropecuários usando o software R. Dissertação em Estatística e Experimentação agronômica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brasil.Google Scholar
Silva, P.F.N. & Gadella, B.M. 2006. Detection of damage in mammalian sperm cells. Theriogenology 65: 958–978.Google Scholar
Sousa, A.P.F., Freneau, G.E., Fioravanti, M.C. & Juliano, R.S. 2004. Estudo da espermatogênese pelo sêmen e a morfologia testicular do Gado Pé Duro. Anais do 1° Congresso de Pesquisa, Ensino e Extensão da Universidade Federal de Goiás, 18–24 Outubro 2004, Goiânia (GO), Brasil.Google Scholar
Tangue, S., Van Soom, A., Sterckx, V., Maes, D. & De Kruif, A. 2002. Assessment of different sperm quality parameters to predict in vitro fertility of bulls. Reproduction in Domestic Animals 37: 127–132.Google Scholar
Verstegen, J., Iguer-Ouada, M. & Onclin, K. 2002. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 57: 149–179.Google Scholar