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Genetic differentiation of Indian camel (Camelus dromedarius) breeds using random oligonucleotide primers

Published online by Cambridge University Press:  01 August 2011

S.C. Mehta ,
B.P. Mishra  and
M.S. Sahani
S.C. Mehta
Affiliation:
National Research Centre on Camel, Bikaner 334001, India
B.P. Mishra
Affiliation:
National Bureau of Animal Genetic Resources, Karnal 132001, India
M.S. Sahani
Affiliation:
National Research Centre on Camel, Bikaner 334001, India
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Summary

The camel population in India is facing a severe decline which demands that immediate steps are taken to ensure its conservation. Characterisation is an integral part of the conservation program. The Polymerase Chain Reaction-Randomly Amplified Polymorphic DNA profile of unrelated camels of the Bikaneri (29), Jaisalmeri (30) and Kachchhi (18) breeds were analyzed. Reproducible polymorphic bands with varying frequencies among the three breeds of camel were obtained with five oligonucleotide primers. A total of 75 bands were amplified, of which 27 (36%) were polymorphic. The probability of obtaining identical fingerprints was observed to be the lowest in primer GC-10 (5.7%) followed by OP-08 (8.7%), GT-10 (11.3%), G-2 (15.5%) and G-1 (80%). Breed informative bands were amplified. The maximum genetic variability was observed in the Bikaneri (0.80±0.05) followed by the Kachchhi (0.84±0.06) and the Jaisalmeri (0.87±0.05) breeds. The inter-breed genetic distance estimates indicated a closer relationship in the Bikaneri-Kachchhi camels, (0.075), followed by the Jaisalmeri-Kachchhi (0.106) and Bikaneri-Jaisalmeri (0.132) breeds. A similar genetic relationship was observed when the degree of population subdivision was measured between the Bikaneri-Kachchhi (0.529), Jaisalmeri-Kachchhi (0.558) and Bikaneri-Jaisalmeri (0.566) breeds.

Resumen

La población de camélidos en la India se enfrenta con un declive importante que requiere iniciar con una rápida intervención en vistas de su conservación. Un parte integral del programa de conservación está representado por la caracterización. Se ha analizado el perfil de ADN polimórfico amplificado casualmente de la cadena de reacción de polimerasa en camélidos sin relación tales las razas Bikaneri (29), Jaisalmeri (30), y Kachchhi (18). Las bandas polifórmicas reproducibles con frecuencias variantes entre las tres razas we obtuvieron con cinco oligonucleotidos primarios. Un total de 75 bandas fueron amplificadas, de las cuales 27 (el 36%) resultaron polimórficas. La probabilidad de obtener huellas idénticas fue inferior en el primer GC-10 (5,7%), seguido por OP-08 (8,7%), GT-10 (11,3%), G-2 (15,5%) y G-1 (80%). Las bandas de información de raza fueron amplificadas. El máximo de variabilidad genética se observó en la raza Bikaneri (0,80±0,05) seguida por la raza Kachchhi (0,85±0,06) y la Jaisalmeri (0,87±0,05). La distancia genética estimada entre razas indica una relación estrecha entre las razas Bikaneri y Kachchhi (0,075), seguida por Jaisalmeri-Kachchhi (0,106) y Bikaneri-Jaisalmeri (0,132). Se observó una relación genética similar cuando el grado de subdivisión de la población fue medido entre Bikaneri-Kachchhi (0,529), Jaisalmeri-Kachchhi (0,558) y Bikaneri-Jaisalmeri (0,566).

Keywords

CharacterisationRAPDCamelus dromedariesStatistical analysisGenetic distanceSimilarities
Type
Research Articles
Information
Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales , Volume 39 , April 2006 , pp. 77 - 88
Copyright
Copyright © Food and Agriculture Organization of the United Nations 0000

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