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Comparative genomics and proteomics to study tissue-specific response and function in natural Mycobacterium bovis infections

Published online by Cambridge University Press:  13 August 2007

Victoria Naranjo ,
Christian Gortazar ,
Margarita Villar  and
José de la Fuente
Victoria Naranjo
Affiliation:
Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain
Christian Gortazar
Affiliation:
Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain
Margarita Villar
Affiliation:
Instituto de Tecnologías Química y Medioambiental ITQUIMA, University of Castilla La Mancha, Campus Universitario s/n, 13071 Ciudad Real, Spain
José de la Fuente*
Affiliation:
Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain Center for Veterinary Health Sciences, Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA
*
*Corresponding author. E-mail: jose_delafuente@yahoo.com or jose.de_la_fuente@okstate.edu
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Abstract

Bovine tuberculosis (bTB) is an established zoonotic disease which affects cattle and wildlife worldwide and new strategies are required to control and eradicate the disease. The European wild boar (Sus scrofa) is a major reservoir of bTB in Spain. The objective of this paper was to review tissue-specific response and function of mandibular lymph nodes (MLN) and oropharyngeal tonsils (OT) in European wild boar naturally infected with Mycobacterium bovis. Genomics and proteomics data were used to compare differential gene expression and global protein patterns in OT and MLN of M. bovis-infected and uninfected European wild boar and the results were analyzed considering previous reports of experimental infections in laboratory and domestic animals. The results showed tissue-specific differences in OT and MLN in response to M. bovis infection. Tissue-specific differences in gene expression and protein profiles suggested different functions for OT and MLN during mycobacterial infection and provided information to characterize the pathobiology of M. bovis infection in European wild boar with important implications for the control of bTB in Spain. The characterization of molecular events in tissues that play different roles during mycobacterial infection in naturally infected individuals may be relevant to understand the pathobiology of M. bovis infection and to design effective strategies for the control of bTB in wildlife reservoirs.

Keywords

bovine tuberculosiswild boarmycobacteriaIberian red deervaccine
Type
Review Article
Information
Animal Health Research Reviews , Volume 8 , Issue 1 , June 2007 , pp. 81 - 88
Copyright
Copyright © Cambridge University Press 2007

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