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Molecular serogrouping of Escherichia coli

Published online by Cambridge University Press:  22 February 2018

Chitrita DebRoy ,
Pina M. Fratamico  and
Elisabeth Roberts
Chitrita DebRoy*
Affiliation:
Department of Veterinary and Biomedical Sciences, E. coli Reference Center, The Pennsylvania State University, University Park, PA, USA
Pina M. Fratamico
Affiliation:
U.S. Department of Agriculture, Eastern Regional Research Center, Agricultural Research Service, Wyndmoor, PA, USA
Elisabeth Roberts
Affiliation:
Department of Veterinary and Biomedical Sciences, E. coli Reference Center, The Pennsylvania State University, University Park, PA, USA
*
*Corresponding author. E-mail: rcd3@psu.edu
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Abstract

O-antigens present on the surface of Escherichia coli provide antigenic specificity for the strain and are the main components for O-serogroup designation. Serotyping using O-group-specific antisera for the identification of E. coli O-serogroups has been traditionally the gold-standard for distinguishing E. coli strains. Knowledge of the O-group is important for determining pathogenic lineage, classifying E. coli for epidemiological studies, for determining virulence, and for tracing outbreaks of diseases and sources of infection. However, serotyping has limitations, as the antisera generated against each specific O-group may cross-react, many strains are non-typeable, and others can autoagglutinate or be rough (lacking an O-antigen). Currently, the nucleotide sequences are available for most of the 187 designated E. coli O-groups. Public health and other laboratories are considering whole genome sequencing to develop genotypic methods to determine O-groups. These procedures require instrumentation and analysis that may not be accessible and may be cost-prohibitive at this time. In this review, we have identified unique gene sequences within the O-antigen gene clusters and have targeted these genes for identification of O-groups using the polymerase chain reaction. This information can be used to distinguish O-groups by developing other platforms for E. coli diagnostics in the future.

Keywords

serotypinggenotypingPCRsequencingO-antigen gene cluster
Type
Review Article
Information
Animal Health Research Reviews , Volume 19 , Issue 1 , June 2018 , pp. 1 - 16
Copyright
Copyright © Cambridge University Press 2018 

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