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Correlation of serum GH and IGF-1 with satellite cell responsiveness in Targhee rams

Published online by Cambridge University Press:  02 September 2010

M. V. Dodson ,
K. L. Hossner ,
J. L. Vierck ,
B. Mathison  and
E. Krabbenhoft
M. V. Dodson
Affiliation:
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman WA 99164-6320, USA
K. L. Hossner
Affiliation:
Department of Animal Sciences, Colorado State University, Fort Collins CO 80523, USA
J. L. Vierck
Affiliation:
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman WA 99164-6320, USA
B. Mathison
Affiliation:
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman WA 99164-6320, USA
E. Krabbenhoft
Affiliation:
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman WA 99164-6320, USA
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Abstract

This study was performed to assess the relationship between serum growth hormone (GH) and insulin-like growth factor 1 (IGF-1) levels, slaughter weight, and myogenic satellite cell growth kinetics of Targhee rams. Serum was collected from 19 rams at 15-min intervals over a period of 4h. Radioimmunoassays of serum samples for GH revealed considerable variation (within individual rams) over the sampling period, with mean values ranging from 0·63 to 4·88 μg/l (mean overall 2·3 (s.e. 0·33) μg/l; no. = 19). There was no significant correlation between GH levels and slaughter weight (r = −0·11; P > 0·05) at 155 (s.e. 1·08) days. Serum IGF-1 levels of (individual) rams were invariant over the sampling period, with individual means ranging from 62 to 233 μg/l (mean overall of 117 (s.e. 45·6) ugll; no. = 19). IGF-1 was not strongly correlated with slaughter weight (r = +0·35; P > 0·05). Satellite cells were isolated from the left m. semimembranosus of all rams at slaughter and grown in culture to evaluate proliferation amount and differentiation extent. The correlations between serum GH levels and satellite cell proliferation and differentiation in vitro were r = −0·53 (P < 0·05) and r = −0·52 (P < 0·05), respectively. Serum IGF-1 showed no significant correlations to proliferation (r = +0·07; P > 0·05) or to differentiation (r = −0·07; P > 0·05) of the satellite cells. These data suggest that serum GH levels in Targhee rams may not reflect muscle growth potential if correlated to body weight of 155 days. Furthermore, as IGF-1 was not correlated significantly with slaughter weight or to variables of satellite cell proliferation and differentiation, another mode of satellite cell regulation (possibly paracrine controllers) is more likely at play to coordinate the satellite cell involvement in muscle growth in Targhee rams at 155 days.

Keywords

differentiationproliferationsatellite cellssheepsomatomedinsomatotropin
Type
Research Article
Information
Animal Science , Volume 62 , Issue 1 , February 1996 , pp. 89 - 96
Copyright
Copyright © British Society of Animal Science 1996

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References

Allen, R. E., Dodson, M. V., Boxhorn, L. K., Davis, S. L. and Hossner, K. L. 1986. Satellite cell proliferation in response to pituitary hormones. Journal of Animal Science 62: 1596–1601.Google Scholar
Bauman, D. E., Eisemann, J. H. and Currie, W. B. 1982. Hormonal effects on partitioning of nutrients for tissue growth: role of growth hormone and prolactin. Federation Proceedings 41: 2538–2544.Google Scholar
Breier, B. H., Bass, J. J., Butler, J. H. and Gluckman, P.D. 1986. The somatotrophic axis in young steers: influence of nutritional status on pulsitile release of growth hormone and circulating concentrations of insulin-like growth factor 1. Journal of Endocrinology 111: 209.Google Scholar
Buonomo, F. C. and Baile, C. A. 1988. Developmental changes in serum GH, IGF-1, IGF-II and thyroid hormone levels of swine with different mature body weights. Proceedings of the seventieth annual meeting of the Endocrine Society, abstr. no. 877.Google Scholar
Clemmons, D. R., Elgin, R. G., Han, V. K. M., Casella, S. J., D'Ercole, A. J. and Van Wyk, J. J. 1986. Cultured fibroblast monolayers secrete a protein that alters the cellular binding of somatomedin-C/insulinlike growth factor I. Journal of Clinical Investigation 77:1548–1556.CrossRefGoogle ScholarPubMed
Clemmons, D. R. and Underwood, L. E. 1991. Nutritional regulation of IGF-1 and IGF-1 binding proteins. Annual Review of Nutrition 11: 393–412.Google Scholar
Cook, J. J., Haynes, K. M. and Werther, G. A. 1988. Mitogenic effects of growth hormone in cultured human fibroblasts. Journal of Clinical Investigation 81: 206–212.CrossRefGoogle ScholarPubMed
Cottam, Y. H., Blair, H. T., Gallaher, B. W., Purchas, R. W., Breier, B. H., McCutcheon, S. N. and Gluckman, P. D. 1992. Body growth, carcass composition, and endocrine changes in lambs chronically treated with recombinantly derived insulin-like growth factor-I. Endocrinology 130: 2924–2930.CrossRefGoogle ScholarPubMed
Daughaday, W. R., Parker, K. A., Borowsky, S., Trivedi, B. and Kapadia, M. 1982. Measurement of somatomedin-related peptides in fetal, neonatal and maternal rat serum by insulin-like growth factor (IGF) I radioimmunoassay, IGF-II radioreceptor assay (RRA) and multiplication stimulating activity RRA after acid-ethanol extraction. Endocrinology 110: 575.Google Scholar
Davis, M. E., Bishop, M. D., Park, N. H. and Simmen, R. C. M. 1995. Divergent selection for blood serum insulin-like growth factor I concentration in beef cattle. 1. Nongenetic effects. Journal of Animal Science 73:1927–1932.CrossRefGoogle ScholarPubMed
Davis, S. L., Hossner, K. L. and Ohlson, D. L. 1984. Endocrine regulation of growth in ruminants. In Manipulation of growth in farm animals (ed. Roche, J. F. and O'Callaghan, D.), pp. 151–183. Martinus Nijhoff, Boston.Google Scholar
Dodson, M. V., Allen, R. E. and Hossner, K. L. 1985. Ovine somatomedin, multiplication-stimulating activity, and insulin promote skeletal muscle satellite cell proliferation in vitro. Endocrinology 117: 2357.CrossRefGoogle ScholarPubMed
Dodson, M. V., Davis, S. L., Ohlson, D. L. and Ercanbrack, S. K. 1983. Temporal patterns of growth hormone, prolactin and thyrotropin secretion in Targhee rams selected for rate and efficiency of gain. Journal of Animal Science 57: 338.CrossRefGoogle ScholarPubMed
Dodson, M. V., McFarland, D. C., Martin, E. L. and Brannon, M. A. 1986. Isolation of satellite cells from ovine skeletal muscles. Journal of Tissue Culture Methods 10: 233–237.CrossRefGoogle Scholar
Dodson, M. V., McFarland, D., Bandman, E., Dayton, W. R., Yablonka-Reuveni, Z., Green, E., Doumit, M., Bergen, W., Merkel, R., Vierck, J., Velleman, S. and Koumans, J. 1995. Status of satellite cell research in agriculture. Basic and Applied Myology 5: 5–9.Google Scholar
Dodson, M. V., Mathison, B. A. and Mathison, B. D. 1990. Effects of medium and substratum on ovine satellite cell attachment, proliferation and differentiation in vitro. Cell Differentiation and Development 29: 59–66.Google Scholar
Eigenmann, J. E., Amador, A. and Patterson, D. F. 1988. Insulin-like growth factor I levels in proportionate dogs, chondrodystrophic dogs and in giant dogs. Ada Endocrinologica 118: 105–108.Google Scholar
Eigenmann, J. E., Patterson, D. F. and Froesch, E. R. 1984. Body size parallels insulin-like growth factor I levels but not growth hormone secretory capacity. Acta Endocrinologica 106: 448.Google Scholar
Fletcher, T. P., Thomas, G. B., Dunshea, F. R., Moore, L. G. and Clarke, I. J. 1995. IGF feedback effects on growth hormone secretion in ewes: evidence for action at the pituitary but not the hypothalamic level. Journal of Endocrinology 144: 323–331.CrossRefGoogle Scholar
Florini, J. R. 1987. Hormonal control of muscle growth. Muscle and Nerve 10: 577–598.Google Scholar
Froesch, E. R. and Zapf, J. 1985. IGF's/somatomedins: significance for growth. In Endocrine genetics and genetics of growth, pp. 75–89. Alan R. Liss, New York.Google Scholar
Furlanetto, R. W., Underwood, L. E., Van Wyk, J. J. and D'Ercole, A. J. 1977. Estimation of somatomedin-C levels in normals and patients with pituitary disease by radioimmunoassay. Journal of Clinical Investigation 60: 648.CrossRefGoogle ScholarPubMed
Green, H., Morikawa, M. and Nixon, T. 1985. A dual effector theory of growth-hormone action. Differentiation 29: 195–198.CrossRefGoogle ScholarPubMed
Greenwood, F. C., Hunter, W. M. and Glover, J. S. 1963. The preparation of 1311 labelled human growth hormone of high specific radioactivity. Journal of Biochemistry 89: 114–123.CrossRefGoogle Scholar
Grings, E. E., Scarborough, R., Schally, A. V. and Reeves, J. J. 1988. Response to a growth hormone-releasing hormone analog in heifers treated with recombinant growth hormone. Domestic Animal Endocrinology 5: 47–53.Google Scholar
Hart, I. C. and Johnsson, I. D. 1986. Growth hormone and growth in meat producing animals. In Control and manipulation of animal growth (ed. Buttery, P. J., Haynes, N. B. and Lindsay, D. B.), pp. 135–159. Butterworths, London.CrossRefGoogle Scholar
Hathaway, M. R., Hembree, J. R., Pampusch, M. S. and Dayton, W. R. 1991. Effect of transforming growth factor beta-1 on ovine satellite cell proliferation and fusion. Journal of Cellular Physiology 146: 435–441.CrossRefGoogle ScholarPubMed
Hizuka, N., Takano, K., Shizume, K., Asakawa, K., Miyakawa, M., Tanaka, I. and Horikawa, R. 1986. Insulin-like growth factor I stimulates growth in normal rats. European Journal of Pharmacology 125:143.CrossRefGoogle Scholar
Hodgkinson, S. C., Spencer, G. S. G., Bass, J. J., Davis, S. R. and Gluckman, P. D. 1991. Distribution of circulating insulin-like growth factor-I (IGF-I) into tissues. Endocrinology 129: 2085–2093.CrossRefGoogle ScholarPubMed
Holland, M. D., Hossner, K. L., Niswender, G. D. and Odde, K. G. 1988. Validation of a heterologous radioimmunoassay for insulin-like growth factor in bovine serum. Journal of Endocrinology 119: 281–285.CrossRefGoogle ScholarPubMed
Hua, K. M., Ord, R., Kirk, S., Li, Q. J., Hodgkinson, S. C., Spencer, G. S. G., Molan, P. C. and Bass, J. J. 1993. Regulation of plasma and tissue levels of insulin-like growth factor-I by nutrition and treatment with growth hormone in sheep. Journal of Endocrinology 136: 217–224.Google Scholar
Laron, Z. 1983. Somatomedin, insulin, growth hormone and growth: a review. Israel Journal of Medical Sciences 18: 823.Google Scholar
Mathison, B. D., Mathison, B. A., McNamara, J. P. and Dodson, M. V. 1989. Insulin-like growth factor I receptor analysis of satellite cell-derived myotube membranes established from two lines of Targhee rams selected for growth rate. Domestic Animal Endocrinology 6: 191–201.CrossRefGoogle ScholarPubMed
Mauro, A. 1961. Satellite cell of skeletal muscle fibres. Journal of Biophysical and Biochemical Cytology 9: 493–495.Google Scholar
Medrano, J. F. and Bradford, G. E. 1991. Growth performance and plasma insulin-like growth factor I concentrations in sheep selected for high weaning weight. Journal of Animal Science 69: 1912–1918.Google Scholar
Microsoft Corporation. 1990. Excel 3.0 user's guide. Microsoft Corporation, Redmond, WA.Google Scholar
Molnar, G. and Dodson, M. V. 1992. Characterization of sheep semimembranosus muscle and associated satellite cells: expression of fast and slow myosin heavy chain. Basic and Applied Myology 2: 183–190.Google Scholar
Olsen, R. F., Wangsness, P. J., Patton, W. H. and Martin, R. J. 1981. Relationship of serum somatomedin-like activity and fibroblast proliferative activity with age and body weight gain in sheep. Journal of Animal Science 52: 63–68.CrossRefGoogle ScholarPubMed
Purchas, R. W., MacMillan, K. L. and Hafs, H. D. 1970. Pituitary and plasma growth hormone levels in bulls from birth to one year of age. Journal of Animal Science 31: 358.Google Scholar
Roe, J. A., Haji Baba, A. S., Harper, J. M. M. and Buttery, P. J. 1995. Effects of growth factors and gut regulatory peptides on nutrient uptake in ovine muscle cell cultures. Comparative Biochemistry and Physiology 110A: 107–114.Google Scholar
Ronge, H. and Blum, J. 1989. Insulin-like growth factor during growth in bulls. Reproduction, Nutrition, and Development 29: 105–111.Google Scholar
Scanes, C. G., Lazarus, D., Bowen, S., Buonomo, F. C. and Gilbreath, R. L. 1987. Postnatal changes in circulating concentrations of growth hormone, somatomedin C and thyroid hormones in pigs. Domestic Animal Endocrinology 4: 253.Google Scholar
Schoenle, E., Zapf, J., Humbel, R. E. and Froesch, E. R. 1982. Insulin-like growth factor-I stimulates growth in hypophysectomized rats. Nature, London 296: 252.Google Scholar
Statistical Analysis Systems Institute. 1979. User's guide: statistics. Satistical Analysis Systems Institute Inc., Cary, NC.Google Scholar
Straus, D. S. 1994. Nutritional regulation of hormones and growth factors that control mammalian growth. FASEB Journal 8: 6–12.Google Scholar
Tannenbaum, G. S., Guyda, H. J. and Posner, B. I. 1983. Insulin-like growth factors: a role in growth hormone negative feedback and body weight regulation via brain. Science, USA 220: 77–79.Google ScholarPubMed
Van Buul-Offers, S., Ueda, I. and Van den Brande, J. L. 1986. Biosynthetic somatomedin C (SM-C/IGF-I) increases the length and weight of snell-dwarf mice. Pediatric Research 20: 825.CrossRefGoogle ScholarPubMed
Venkateswaran, V., Brackett, E. J., Vierck, J. L., Cloud, J. G. and Dodson, M. V. 1995. Substratum is an important determinant in growth factor regulation of trout-derived satellite cells. Basic and Applied Myology 5: 297–304.Google Scholar
Vierck, J. L., McNamara, J. P., Hossner, K. L. and Dodson, M. V. 1995. Characteristics of ovine skeletal muscle satellite cell strains in a defined culture medium formulated to enhance differentiation: fusion and the IGF-I system. Basic and Applied Myology 5: 11–20.Google Scholar
Zapf, J. and Froesch, E. R. 1986. Insulin-like growth factors/somatomedins: Structure, secretion, biological actions and physiologic role. Hormone Researcli 24: 121.Google Scholar
Zapf, J., Hauri, C. and Froesch, E. R. 1986. Acute metabolic effects and half-lives of intravenously administered insulin-like growth factors I and II in normal and hypophysectomized rats. Journal of Clinical Investigation 77: 1768.CrossRefGoogle Scholar
Zenobi, P. D., Guler, H-P., Zapf, J. and Froesch, E. R. 1988. Insulin-like growth factors in the Gottinger miniature pig. Ada Endocrinologica 117: 343–352.Google Scholar