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Gammaglutamyltransferase activity in buffalo mammary tissue during lactation

Published online by Cambridge University Press:  09 March 2007

M. E. Pero ,
N. Mirabella ,
P. Lombardi ,
C. Squillacioti ,
A. De Luca  and
L. Avallone
M. E. Pero
Affiliation:
Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, University of Naples Federico II, Via Delpino 1, 80137, Napoli, Italy
N. Mirabella
Affiliation:
Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, University of Naples Federico II, Via Delpino 1, 80137, Napoli, Italy
P. Lombardi*
Affiliation:
Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, University of Naples Federico II, Via Delpino 1, 80137, Napoli, Italy
C. Squillacioti
Affiliation:
Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, University of Naples Federico II, Via Delpino 1, 80137, Napoli, Italy
A. De Luca
Affiliation:
Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, University of Naples Federico II, Via Delpino 1, 80137, Napoli, Italy
L. Avallone
Affiliation:
Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, University of Naples Federico II, Via Delpino 1, 80137, Napoli, Italy
*
†Corresponding author. E-mail: pilombar@unina.it
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Abstract

In the present study, the rôle of gammaglutamyltransferase (GGT) during lactation has been investigated in the water buffalo. GGT activity has been evaluated in the mammary tissue at 4 and 6 months after calving and during the non-lactating period. The highest GGT activity levels were found at day 120 (32·57±7·41 U per g) of lactation and were statistically higher than those at 180 (10·76±3·6 U per g) or during the non-lactating period (9·86±7·94 U per g). Histochemistry confirmed these findings and revealed that GGT reactivity was distributed throughout the cytoplasm of alveolar epithelial cells. Such results showed that the GGT production is high during lactation thus supporting the hypothesis that this enzyme plays a rôle in determining milk production in water buffalo by supporting milk protein synthesis.

Keywords

buffaloesγ-glutamyltransferasehistochemistrylactation
Type
Research Article
Information
Animal Science , Volume 82 , Issue 3 , June 2006 , pp. 351 - 354
Copyright
Copyright © British Society of Animal Science 2006

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References

Baumrucker, C. R. and Pocius, P. A. 1978. Gamma-glutamyl transpeptidase in lactating mammary secretory tissue of cow and rat. Journal of Dairy Science 61: 309–314.Google Scholar
Bogin, E., Avidar, Y., Shenkler, S., Israeli, B. A., Spiegel, N. and Cohen, R. 1993. A rapid field test for the determination of colostral ingestion by calves. European Journal of Clinical Chemistry and Clinical Biochemistry 31: 695–699.Google ScholarPubMed
Braun, J. P., Rico, A. G. and Bernard, P. 1978. Blood and tissue distribution of gamma glutamyltransferase in calves. Journal of Dairy Science 61: 596–599.Google Scholar
Cant, J. P., DePeters, E. J. and Baldwin, R. L. 1993. Mammary amino acid utilization in dairy cows fed fat and its relationship to milk protein depression. Journal of Dairy Science 76: 762–774.CrossRefGoogle ScholarPubMed
Chikhi, N., Holic, N., Guellaen, G. and Laperche, Y. 1999. Gamma-glutamyltranspeptidase gene organization and expression: a comparative analysis in rat, mouse, pig and human species. Comparative Biochemistry and Physiology, Part B 122: 376–380.CrossRefGoogle ScholarPubMed
Fiala, S., Trout, E. C. and Teague, C. A. and Fiala, E. S. 1980. Gamma-glutamyltransferase, a common marker of human epithelial tumors?. Cancer Detection Prevention 3:471–485.Google ScholarPubMed
Hanigan, M. H., Henry, F. and Frierson, J. R. 1996. Immunohistochemical detection of gamma-glutamyltramspeptidase in normal human tissue. Journal of Histochemistry and Cytochemistry 44: 1101–1109.CrossRefGoogle ScholarPubMed
Janosi, S. and Baltay, Z. 2004. Correlation among the somatic cell count of individual bulk milk result of California Mastitis Test and bacteriological status of udder in dairy cows. Acta Veterinaria Hungarica 52: 173–183.CrossRefGoogle ScholarPubMed
Johnston, S. L., Kitson, K. E., Tweedie, J. W., Davis, S. R. and Lee, J. 2004. Gamma-glutamyl transpeptidase inhibition suppresses milk protein synthesis in isolated ovine mammary cells. Journal of Dairy Science 87: 321–329.CrossRefGoogle ScholarPubMed
Lee, W. J., Hawskins, R. A., Peterson, D. R. and Viña, J. R. 1996. Role of oxoproline in the regulation of neutral amino acid transport across the blood-brain barrier. Journal of Biological Chemistry 32:19129–19133.CrossRefGoogle Scholar
Lombardi, P., Avallone, L.d', Angelo, A. and Bogin, E. 1996. Gamma-glutamyltransferase and serum proteins in buffalo calves following colostral ingestion. European Journal of Clinical Chemistry and Clinical Biochemistry 34: 965–968.Google ScholarPubMed
Lombardi, P., Avallone, L., Pagnini, U., d’ Angelo, D. and Bogin, E. 2001. Evaluation of buffalo colostrum quality by estimation of enzyme activity levels. Journal of Food Protection 64: 1265–1267.CrossRefGoogle ScholarPubMed
Persijn, J. P. and van der Slik, W. 1976. A new method for the determination of gamma-glutamyltransferase in serum. Journal of Clinical Chemistry and Clinical Biochemistry 14: 421–427.Google ScholarPubMed
Pocius, P. A., Baumrucker, C. R., McNamara, J. P. and Bauman, D. E. 1980. Gamma-glutamyl transpeptidase in rat mammary tissue. Activity during lactogenesis and regulation by prolactin. Biochemical Journal 188: 565–568.CrossRefGoogle ScholarPubMed
Puente, J., Varas, M. A., Beckaus, G. and Sapag-Hagar, M. 1979. Gamma-glutamyltranspeptidase activity and cyclic AMP levels in rat liver and mammary gland during the lactogenic cycle and in the oestradiol-progesterone pseudo-induced pregnancy. FEBS Letters 99: 215–218.CrossRefGoogle ScholarPubMed
Rico, A. G., Braun, J. P. and Bernard, P. 1977. Blood and tissue distribution of gammaglutamyltransferase in the cow. Journal of Dairy Science 60: 1283–1287.CrossRefGoogle ScholarPubMed
Rutenburg, A. M., Kim, H., Fischbein, J. W., Hanker, J. S., Wassenburg, H. L. and Seligman, A. M. 1969. Histochemical and ultrastructural demonstration of gamma-glutamyl transpeptidase activity. The Journal of Histochemistry and Cytochemistry 17: 517–526.Google Scholar
Siegrist, S., Laperche, Y., Chobert, M. N., Bulle, F., Nakhasi, H. L. and Guellaen, G. 1990. Regulation of mouse mammary-gland gamma-glutamyltranspeptidase mRNA during pregnancy, lactation and weaning. Biochemical Journal 267: 621–624.Google Scholar
Statistical Analysis Systems Institute 1985. SAS user's guide: statistics. SAS Institute Inc., Cary, NC.Google Scholar
Taniguchi, N., Iizuka, S., Zhe, Z. N., House, S., Yokosawa, N., Ono, M., Kinoshita, K., Makita, A. andSekiya, C. 1985. Measurement of human serum immunoreactive gamma-glutamyl transpeptidase in patients with malignant tumors using enzyme-linked immunosorbent assay. Cancer Research 45: 5835–5839.Google ScholarPubMed
Viña, J. R., Garcia, C. and Barber, T. 2001. Regulation of amino acid metabolism during lactation. Recent Research Developments in Nutrition 4: 101–111.Google Scholar
Viña, J. R., Palacin, M., Puertes, I. R. and Viña, J. 1989. Role of the gamma-glutamyl cycle in the regulation of amino acid translocation. American Physiological Society 257: 916–922.Google Scholar
Viña, J. R., Puertes, I. R., Montoro, J. B., Saez, G. T. and Viña, J. 1985. Gamma-glutamyl-amino acids as a signals for the hormonal regulation of amino acid uptake by mammary gland of the lactating rat. Biology of Neonate 48: 250–256.CrossRefGoogle ScholarPubMed
Viña, J., Puertes, I. R., Montoro, J. B. and Viña, J. R. 1983. Effect of specific inhibition of gamma-glutamyltranspeptidase on amino acid uptake by mammary gland of lactating rat. FEBS Letters 159: 119–122.CrossRefGoogle ScholarPubMed