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Ultrasound and hormone profiles to monitor follicular activity in cattle after treatment with high doses of progesterone and oestradiol

Published online by Cambridge University Press:  18 August 2016

W. R. Ward ,
A. Y. Ribadu ,
D. Harvey  and
H. Dobson
W. R. Ward
Affiliation:
Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool, Leahurst, Neston CH64 7TE, UK
A. Y. Ribadu
Affiliation:
Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool, Leahurst, Neston CH64 7TE, UK
D. Harvey
Affiliation:
Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool, Leahurst, Neston CH64 7TE, UK
H. Dobson
Affiliation:
Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool, Leahurst, Neston CH64 7TE, UK
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Abstract

To mimic the abnormal patterns of follicular growth in the early post-partum period, heifers were injected with 15 mg oestradiol and 37•5 mg progesterone every 12 h for 7 days starting on day 15 of the oestrous cycle. Control and treated animals (no. = 8 per group) were monitored by daily ultrasonography and plasma hormone measurements. Five treated animals had quiescent ovaries for 11 days during which period oestradiol concentrations exceeded 30 pg/ml. After this, the first post-treatment follicular wave produced a normal ovulatory follicle. The other three heifers had quiescent ovaries for 17 days during exposure to high oestradiol values but this was followed by the formation of persistent follicles. The prolonged exposure to oestradiol probably disrupted normal LH surges in the latter animals. Growth rates and internal diameters for normal, ovulatory and prolonged persistent follicles were similar for the first 6 days after emergence, but the persistent follicles grew larger (P < 0·05) than normal follicles over the next 20 days. Luteinization of a subsequent persistent follicle was observed in one heifer. For 2 to 3 days prior to follicular growth, FSH concentrations were similar in controls, and heifers with ovulatory or persistent follicles (P > 0·05). When follicles first emerged and for the next 4 days, oestradiol concentrations were greater in follicles which later persisted (P < 0·05). For the second half of the life-span of persistent follicles, oestradiol concentrations were basal, thus the structure remained for many days after endocrine function had declined. These observations allow new interpretation of previously confusing findings in subfertile cattle presented to veterinarians.

Keywords

luteinizationovulation
Type
Research Article
Information
Animal Science , Volume 71 , Issue 3 , December 2000 , pp. 577 - 583
Copyright
Copyright © British Society of Animal Science 2000

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References

Beard, A. P., Hunter, M. G. and Lamming, G. E. 1994. Quantitative control of oxytocin-induced PGF2a release by progesterone and oestradiol in ewes. Journal of Reproduction and Fertility 100: 143—150.Google Scholar
Carriere, P. D., Amaya, D. and Lee, B. 1995. Ultrasonography and endocrinology of ovarian dysfunction induced in heifers with estradiol valerate. Theriogenology 43: 1061—1076.Google Scholar
Cook, D. L., Parfect, J. R., Smith, C. A., Moss, G. E., Youngquist, R. S. and Garverick, H. A. 1990a. Secretory patterns of LH and FSH during development and hypothalamic and hypophyseal characteristics following development of steroid-induced ovarian follicular cysts in dairy cattle. Journal of Reproduction and Fertility 91: 19—28.Google Scholar
Cook, D. L., Smith, C. A., Parfect, J. R., Youngquist, R. S., Brown, E. M. and Garverick, H. A. 1990b. Fate and turnover of ovarian follicular cysts in dairy cattle. Journal of Reproduction and Fertility 90: 37—46.Google Scholar
Dobson, H. 1978. Radioimmunoassay of FSH in the plasma of post-partum dairy cows. Journal of Reproduction and Fertility 52: 45—49.Google Scholar
Dobson, H. and Nanda, A. S. 1992. Reliability of cyst diagnosis and effect of energy status on LH released by oestradiol or GnRH in cows with ovarian cysts. Theriogenology 37: 465—472.Google Scholar
Dobson, H., Ribadu, A. Y., Noble, K. M. and Ward, W. R. 2000. Ultrasound and hormone profiles of ACTH-induced persistent follicles (cysts) in cattle. Journal of Reproduction and Fertility In press.Google Scholar
Dobson, H., Tebble, J. E., Ozturk, M. and Smith, R. F. 1999. Effect of transport on pulsatile LH release in ovariectomised ewes in different seasons of the year. Journal of Reproduction and Fertility 117: 213—222.Google Scholar
Douthwaite, R. and Dobson, H. 2000. Bovine cystic ovarian disease: a comparison of different methods of diagnosis, and assessment of treatment with progesterone releasing intra vaginal device (PRID). Veterinary Record In press.Google Scholar
Garverick, H. A. 1997. Ovarian follicular cysts in cattle. Journal of Dairy Science 80: 995—1004.Google Scholar
Hamilton, S. A., Garverick, H. A., Keisler, D. H., Xu, Z. Z., Loos, K., Youngquist, R. S. and Salfen, B. E. 1995. Characterisation of ovarian follicular cysts and associated endocrine profiles in dairy cows. Biology of Reproduction 53: 890—898.Google Scholar
Harvey, D., Noble, K. M. and Dobson, H. 1996. Structure and function of persistent follicles in cattle. Animal Science 62: 690 (abstr.).Google Scholar
Kastelic, J. P., Pierson, R. A. and Ginther, O. J. 1990. Ultrasonic morphology of corpora lutea and central luteal cavities during the estrous cycle and early pregnancy in heifers. Theriogenology 34: 487—492.Google Scholar
Kawate, N., Inaba, T. and Mori, J. 1990. A quantitative comparison in the bovine of steroids and gonadotrophin receptors in follicular and luteal cysts. Animal Reproduction Science 23: 273—281.Google Scholar
Nanda, A. S., Ward, W. R. and Dobson, H. 1989. Relationship between milk yield and cystic ovarian disease in cattle. British Veterinary Journal 145: 39—45.Google Scholar
Nanda, A. S., Ward, W. R., Williams, P. C. W. and Dobson, H. 1988. A retrospective analysis of the efficacy of different hormone treatments of cystic ovarian disease in cattle. Veterinary Record 122: 155—158.Google Scholar
Ozturk, M., Smith, R. F. and Dobson, H. 1998. Effect of prolonged exposure to oestradiol on subsequent LH secretion in ewes. Journal of Reproduction and Fertility 114: 1—9.Google Scholar
Phogat, J. B., Smith, R. F. and Dobson, H. 1997. The influence of stress on the neuroendocrine control of the hypothalamic-pituitary-ovarian axis. Veterinary Bulletin 67: 553—567.Google Scholar
Ribadu, A. Y., Dobson, H. and Ward, W. R. 1993. Ultrasound in the diagnosis and treatment of ovarian cysts. Cattle Practice 1: 400—413.Google Scholar
Ribadu, A. Y., Ward, W. R. and Dobson, H. 1994. Comparative evaluation of ovarian structures in cattle by palpation per rectum, ultrasonography and plasma progesterone concentration. Veterinary Record 135: 452—457.Google Scholar
Savio, J. D., Boland, M. P., Hynes, N. and Roche, J. F. 1990. Resumption of follicular activity in the early postpartum period of dairy cows. Journal of Reproduction and Fertility 88: 569—579.Google Scholar