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Adipocyte size and lipogenic enzyme activities in different adipose tissue depots in steers of local Spanish breeds

Published online by Cambridge University Press:  18 August 2016

J. A. Mendizabal ,
P. Albertí ,
P. Eguinoa ,
A. Arana ,
B. Soret  and
A. Purroy
J. A. Mendizabal
Affiliation:
Departamento de Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
P. Albertí
Affiliation:
Servicio de Investigación Agroalimentaria, Diputación General de Aragón, Apartado 727, 50080 Zaragoza, Spain
P. Eguinoa
Affiliation:
Departamento de Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
A. Arana
Affiliation:
Departamento de Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
B. Soret
Affiliation:
Departamento de Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
A. Purroy
Affiliation:
Departamento de Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
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Abstract

The effect of genotype on the size of adipocytes and on the lipogenic enzyme activities in different fat depots in Asturiana, Avilena, Morucha, Parda Alpina, Pirenaica, Retinta and Rubia Gallega steers was studied (six animals per breed). Steers were weaned at about 6 to 8 months of age and 220 to 260 kg live weight (LW). During the fattening period the steers were given commercial concentrates and straw, both ad libitum, until slaughter at 470 kg average LW. Adipocyte size and activity of the following lipogenic enzymes were studied: glycerol 3-phosphate dehydrogenase (G 3-PDH); fatty acid synthase (FAS); NADP-malate dehydrogenase (MD), glucose 6-phosphate dehydrogenase (G 6-PDH) and NADP-isocitrate dehydrogenase (ICDH) in the omental, perirenal, subcutaneous and intermuscular adipose depots. Also the perirenal fat depot was removed and weighed and the 10th rib was dissected. Differences between breeds were significant for the amount of fat in the different adipose depots (P < 0·001), for adipocyte size (except in intermuscular depot) and for G 3-PDH, FAS, MD, ICDH (P < 0·001) and G 6-PDH (P < 0·01). In general, steers of breeds that had more fat also had bigger adipocytes and higher lipogenic enzyme activities. In this way, Asturiana breed (lean breed) had smaller adipocytes and lower lipogenic enzyme activities than Morucha breed (fatter breed). Finally, adipocytes from the perirenal depot were the biggest and showed the highest G 3-PDH and FAS activities while adipocytes from the intermuscular depot had the smallest size and the lowest lipogenic activities.

Keywords

adipocytesadipose tissueenzymesgenotypessteers
Type
Research Article
Information
Animal Science , Volume 69 , Issue 1 , August 1999 , pp. 115 - 121
Copyright
Copyright © British Society of Animal Science 1999

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