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Dietary carbohydrates with different rates of fermentation affect fermentation end-product profiles in different sites of gastro-intestinal tract of weaning piglet

Published online by Cambridge University Press:  13 March 2007

A. Awati ,
B. A. Williams ,
M.W. Bosch  and
M.W.A. Verstegen
A. Awati
Affiliation:
Animal Nutrition Group, Wageningen University and Research Centre, PO Box 338, 6700 AH, Wageningen, The Netherlands
B. A. Williams
Affiliation:
Animal Nutrition Group, Wageningen University and Research Centre, PO Box 338, 6700 AH, Wageningen, The Netherlands
M.W. Bosch
Affiliation:
Animal Nutrition Group, Wageningen University and Research Centre, PO Box 338, 6700 AH, Wageningen, The Netherlands
M.W.A. Verstegen
Affiliation:
Animal Nutrition Group, Wageningen University and Research Centre, PO Box 338, 6700 AH, Wageningen, The Netherlands
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Abstract

An in vivo experiment was conducted to examine changes in fermentation end-products in the gastro-intestinal tract (GIT) of weaning piglets by the inclusion of fermentable carbohydrates in the diet. The experiment was repeated in three replicates of 36 piglets. Piglets were raised free of antibiotics and creep feeding prior to weaning at 4 weeks of age. Each replicate was conducted over a period of 10 days. The piglets were offered one of two dietary treatments: control diet (CON), and fermentable carbohydrate enriched diet (CHO); and were subjected to one of the two fasting treatments (i) fasting for 2 days in the beginning of the experimental period and (ii) non-fasting. Piglets were slaughtered on the 1st, 4th and 10th day of each period. Digesta samples were collected from: first half of small intestine, second half of small intestine, caecum, and colon. The dry matter, volatile fatty acid (VFA) profile, and ammonia concentrations were analysed. Food intake, growth and food conversion ratio were also recorded. There were no differences in production performances such as growth and food conversion ratio (FCR) between the treatment groups. Concentrations of VFA were significantly higher, while ammonia concentration was significantly lower in the CHO group compared to the CON group in different fermentation sites within the GIT (P<0·001), and on different slaughtering days (P<0·05). Fasting had no effect on fermentation end-products. This study concludes that the addition of fermentable carbohydrates of varying fermentabilities stimulated carbohydrate fermentation, with reduction in protein fermentation along the different parts of GIT studied, in weaning piglets.

Keywords

carbohydratesfermentationpigletsweaning
Type
Research Article
Information
Animal Science , Volume 82 , Issue 6 , December 2006 , pp. 837 - 843
Copyright
Copyright © British Society of Animal Science 2006

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References

Bauer, E. 2002. In-vitro fermentation characteristics of carbohydrate-rich feedstuffs using faeces and mixed microbial populations from the pig large intestine. Ph.D. thesis, Hohenhiem University.Google Scholar
Bauer, E.Williams, B. A.Voigt, C.Mosenthin, R.Verstegen, M. W. A. (2001) Microbial activities of faeces from unweaned and adult pigs, in relation to selected fermentable carbohydrates. Animal Science 73: 313–322CrossRefGoogle Scholar
Bedford, M.R.Apajalahti, J. (2001) Microbial interactions in the response to exogenous enzyme utilization. In Enzymes in farm animal nutrition Bedford, M. R.Partridge, G.G.299–314CAB International, Wallingford.CrossRefGoogle Scholar
Bernalier, A.Dore, J.Durand, M. (1999) Biochemistry of fermentation. In Colonic Microbiota, Nutrition and Health (ed. Gibson, G. R.Roberfroid, M.B.) pp. 37–53Kluwer Academic Publisher The Netherlands.CrossRefGoogle Scholar
Cummings, J.H.Englyst, H. N. (1987) Fermentation in the human large intestine and the available substrates American Journal of Clinical Nutrition 45: 1243–1255CrossRefGoogle ScholarPubMed
Drochner, W. (1991) Digestion of carbohydrates in pigs Digestive physiology in pigs EAAP publication no. 54 367–388Verstegen, M. W. A.Huisman, J.den Hartog, L. A.Wageningen, The Netherlands PudocGoogle Scholar
Ewing, W.N.Cole, D. J. A. (1994) The living gut- an introduction to micro-organisms in nutrition. Context, Dungannon, N. Ireland.Google Scholar
Gaskins, H.R. (2001) Intestinal bacteria and their influence on swine growth. In Swine nutrition (ed. Lewis, A. J. and Southern, L. L.), pp. 585–608. CRC Press LLC, Florida.Google Scholar
Gibson, G.R.Roberfroid, M. B. (1995) Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics Journal of Nutrition 125: 1401–1412CrossRefGoogle ScholarPubMed
Hopwood, D.E.Hampson, D. J. (2003) Interactions between the intestinal microflora, diet and diarrhoea, and their influences on piglet health in the immediate post-weaning period Weaning the pig: concepts and consequencesa 199–218Pluske, J. R. L.Dividich, J.Verstegen, M. W. A.Wageningen, The Netherlands Wageningen Academic PublishersGoogle Scholar
International Standards Organization (1978) International Organization for Standardization: animal feeding stuffs – determination of crude ash (ISO 5984). ISO, Geneva, Switzerland.Google Scholar
International Standards Organization (1999) International Organization for Standardization: animal feeding stuffs – determination of moisture and other volatile matter content (ISO 6496). ISO, Geneva, Switzerland.Google Scholar
Jensen, B.B.Jorgensen, H. (1994) Effect of dietary fiber on microbial activity and microbial gas production in various regions of the gastrointestinal tract of pigs Applied and Environmental Microbiology 60: 1897–1904CrossRefGoogle ScholarPubMed
Knudsen, K.E.B.Hansen, I. (1991) Gastrointestinal implications in pigs of wheat and oat fractions: 1. Digestibility and bulking properties of polysaccharides and other major constituents British Journal of Nutrition 65: 217–232CrossRefGoogle Scholar
Konstantinov, S.R.Zhu, W. Y.Williams, Barbara A.Tamminga, S. d.Vos, W. M. Akkermans, A. D. L. (2003) Effect of fermentable carbohydrates on piglet faecal bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA Fems Microbiology Ecology 43: 225–235CrossRefGoogle ScholarPubMed
Konstantinov, S.R.Awati, A.Smidt, H.Williams, B. A.Akkermans, A. D. L. de Vos, W. M. (2004) Specific response of a novel and abundant lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets Applied and Environmental Microbiology 70: 3821–3830CrossRefGoogle ScholarPubMed
Low, A.G.Partridge, I. G.Sambrook, I. E. (1978) Studies on digestion and absorption in the intestines of growing pigs. 2. Measurements of the flow of dry matter, ash and water British Journal of Nutrition 39: 515–526CrossRefGoogle ScholarPubMed
Macfarlane, S.Macfarlane, G. T. (2003) Regulation of short chain fatty acid production Proceedings of the Nutrition Society 62: 67–72CrossRefGoogle ScholarPubMed
Martinez-Puig, D.Perez, J. F.Castillo, M.Andaluz, A.Anguita, M.Morales, J.Gasa, J. (2003) Consumption of raw potato starch increases colon length and faecal excretion of purine bases in growing pigs Journal of Nutrition 133: 134–139CrossRefGoogle Scholar
Statistical Analysis Systems Institute (1990) SAS user's guide: statistics. (version 6 edition). SAS Institute Inc, Cary, NC.Google Scholar
Searle, P.L. (1984) The berthelot or indophenol reaction and its use in the analytical chemistry of nitrogen. A review. Analyst 109: 549–568CrossRefGoogle Scholar
Van der Waaij, D. (1989) The ecology of the human intestine and its consequences for the overgrowth of pathogens such a Clostridium difficile Annual Reviews in Microbiology 43: 69–87CrossRefGoogle ScholarPubMed
Voragen, A.G.J.Schols, H. A. Searle-van Leeuwen, M. F.Beldman, G.Rombouts, F. M. (1986) Analysis of oligomeric and monomeric saccharides from enzymatically degraded polysaccharides by high-performance liquid chromatography Journal of Chromatography 370: 113–120CrossRefGoogle Scholar
Williams, B.A.Bosch, M. W.Boer, H.Verstegen, M. W. A.Tamminga, S. (2005) An in vitro batch culture method to assess potential fermentability of ingredients for monogastric diets Animal Feed Science and Technology 123–124: 445–462CrossRefGoogle Scholar
Williams, B.A.Verstegen, M. W. A.Tamminga, S. (2001) Fermentation in the large intestine of single-stomached animals and its relationship to animal health Nutrition Research Reviews 14: 207–227CrossRefGoogle ScholarPubMed