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Differentiation in vitro of omental and subcutaneous pre-adipocytes from Spanish Lacha and Rasa Aragonesa sheep

Published online by Cambridge University Press:  18 August 2016

A. Arana ,
R. G. Vernon ,
P. Eguinoa ,
B. Soret ,
J. A. Mendizabal  and
A. Purroy
A. Arana*
Affiliation:
Departamento Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
R. G. Vernon
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
P. Eguinoa
Affiliation:
Departamento Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
B. Soret
Affiliation:
Departamento Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
J. A. Mendizabal
Affiliation:
Departamento Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
A. Purroy
Affiliation:
Departamento Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain
*
E-mail:aarana@unavarra.es
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Abstract

Factors responsible for breed- and depot-specific differences in the development of lipogenic enzymes, and hence lipogenic capacity of adipocytes, in sheep adipose tissue have been investigated using a serum-free cell culture system. Effects of insulin, tri-iodothyronine and exogenous lipid on the development in vitro of the lipogenic enzymes glycerol 3-phosphate dehydrogenase (G3PDH), fatty acid synthetase (FAS), NADP-malate dehydrogenase (ME), glucose 6-phosphate dehydrogenase (G6PDH), and isocitrate dehydrogenase (ICDH) in omental and subcutaneous pre-adipocytes from Lacha and Rasa Aragonesa lambs were investigated. Addition of insulin plus tri-iodothyronine caused pre-adipocyte differentiation, which was enhanced by addition of a lipid supplement. G3PDH activities achieved by differentiation of pre-adipocytes in vitro were similar to those found in vivo; furthermore after differentiation in vitro adipocytes from Rasa Aragonesa lambs had a greater G3PDH activity than adipocytes from Lacha lambs, as found in vivo. In contrast activities of FAS, G6PDH and ME achieved by differentiation in vitro were much greater than those found previously in vivo. While breed- and depot-specific changes in G6PDH observed after differentiation in vitro were similar to those observed in vivo, changes in FAS induced in vitro differed from those found during development in vivo. The study shows that pre-adipocytes from Rasa Aragonesa and Lacha lambs have intrinsic depot- and breed-specific differences in their ability to differentiate and express lipogenic enzymes. The combination of insulin, tri-iodothyronine and a lipid supplement appears to be sufficient to account for in vivo G3PDH activities but other factors are required to explain activities of FAS, G6PDH and ME found in vivo.

Keywords

adipose tissuecell cultureenzymessheep
Type
Growth, development and meat science
Information
Animal Science , Volume 74 , Issue 3 , June 2002 , pp. 469 - 476
Copyright
Copyright © British Society of Animal Science 2002

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References

Ailhaud, G. 1999. Cell surface receptors, nuclear receptors and ligands that regulate adipose tissue development. Clinica and Chimica Acta 286: 181–190.CrossRefGoogle ScholarPubMed
Ailhaud, G., Amri, E. and Negrel, R. 1992. Cellular and molecular aspects of adipose tissue development. Annual Review of Nutrition 12: 201–233.CrossRefGoogle ScholarPubMed
Amri, E. Z., Ailhaud, G. and Grimaldi, P. A. 1994. Fatty acids as signal transducing molecules: involvement in differentiation of preadipose cells to adipose cells. Journal of Lipid Research 35: 930–937.CrossRefGoogle ScholarPubMed
Arana, A., Soret, B., Mendizabal, J. A., Corroza, M., Eguinoa, P. and Purroy, A. 1998. Changes in adipose tissue accumulation in Rasa Aragonesa breed lambs during growth and fattening. Animal Science 66: 409–413.Google Scholar
Björntorp, P., Karlson, M., Petterson, P. and Sypniewska, M. 1980. Differentiation and function of rat adipocyte precursor cells in primary culture. Journal of Lipid Research 21: 714–728.CrossRefGoogle ScholarPubMed
Broad, T. E. and Ham, R. G. 1983. Growth and adipose differentiation of sheep preadipocyte fibroblasts in serum-free medium. European Journal of Biochemistry 135: 33–39.CrossRefGoogle ScholarPubMed
Casteilla, L., Nouguès, J., Reyne, Y. and Ricquier, D. 1991. Differentiation of ovine brown adipocyte precursor cells in a chemically defined serum-free medium. European Journal of Biochemistry 198: 195–199.Google Scholar
Glock, G. E. and McLean, P. 1953. Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochemical Journal 55: 400–408.Google Scholar
Gregoire, F. M., Smas, C. M. and Sul, H. S. 1998. Understanding adipocyte differentiation. Physiological Review 78: 783–809.Google Scholar
Halestrap, A. P. and Denton, R. M. 1973. Insulin and the regulation of adipose tissue acetyl CoA carboxylase. Biochemical Journal 105: 529–536.Google Scholar
Kaiser, K. and Murray, N. E. 1985. The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries. In DNA cloning, volume 1 (ed. Glover, D.), pp. 1–47. IRL Press, Oxford.Google Scholar
Kempster, A. J. 1980-81. Fat partition and distribution in the carcass of cattle, sheep and pigs: a review. Meat Science 5: 83–98.Google Scholar
Mandrup, S. and Lane, M. D. 1997. Regulating adipogenesis. Journal of Biological Chemistry 272: 5367–5370.Google Scholar
Ochoa, S. 1955. Malic enzyme. Methods of Enzymology 1: 739–748.CrossRefGoogle Scholar
Plaut, G. W. E. 1962. Isocitric dehydrogenase (TPN-linked) from pig heart (revised procedure). Methods of Enzymology 5: 645–654.Google Scholar
Purroy, A., Mendizabal, J. A., Soret, B., Arana, A. and Mendizabal, F. J. 1997. Changes in cell number and size and in lipogenic enzyme activity in adipose tissues during growth and fattening of Lacha (Manech) lambs. Annales de Zootechnie 46: 309–319.Google Scholar
Saleh, J., Christou, N. and Cianflone, K. 1999. Regional specificity of ASP binding in human adipose tissue. American Journal of Physiology 276: E815–E821.Google ScholarPubMed
Soret, B., Lee, H.-J., Finley, E., Lee, S. C. and Vernon., R. G. 1999. Regulation of sheep subcutaneous and omental pre-adipocytes in culture. Journal of Endocrinology 161: 517–524.Google Scholar
Soret, B., Mendizabal, J. A., Arana, A., Purroy, A. and Eguinoa, P. 1998. Breed effects on cellularity and lipogenic enzymes in growing Spanish lambs. Small Ruminant Research 29: 103–112.Google Scholar
Statistical Packages for the Social Sciences. 1998. Advanced statistics, version 7·5 for Windows. SPSS, Inc., Chicago.Google Scholar
Travers, M. T. and Barber, M. C. 1999. Insulin-glucocorticoid interactions in the regulation of acetyl-coA carboxylase- transcript diversity in ovine adipose tissue. Journal of Molecular Endocrinology 22: 71–79.CrossRefGoogle ScholarPubMed
Vernon, R. G. 1975. Effect of dietary safflower oil upon lipogenesis in neonatal lamb. Lipids 10: 284–289.CrossRefGoogle ScholarPubMed
Vernon, R. G. 1980. Lipid metabolism in the adipose tissue of ruminant animals. Progress in Lipid Research 19: 23–106.Google Scholar
Vernon, R. G. 1986. The growth and metabolism of adipocytes. In Control and manipulation of animal growth (ed. P. J. Buttery, Hayne, N. B. and Lindsay, D. B.), pp. 67–83. Butterworths, London.Google Scholar
Vernon, R. G. 1992. Control of lipogenesis and lipolysis. In The control of fat and lean deposition (ed. Buttery, P. J. Boorman, K. N. and Lindsay, D. B.), pp. 59–81. Butterworth Heinemann, Oxford.CrossRefGoogle Scholar
Vernon, R. G., Faulkner, A., Finley, E., Pollock, H. and Taylor, E. 1987. Enzymes of glucose and fatty acid metabolism of liver, kidney, skeletal muscle, adipose tissue and mamary gland of lactating and non-lactating sheep. Journal of Animal Science 64: 1395–1411.CrossRefGoogle Scholar
Vierck, J. L., McNamara, J. P. and Dobson, M. V. 1996. Proliferation and differentiation of progeny of ovine unilocular fat cells (adipofibroblasts). In vitro Cellular and Development Biology-Animal 32: 564–572.Google Scholar
Ward, R. J., Travers, M. T., Richards, S.E., Vernon, R. G., Salter, A. M., Buttery, P. J. and Barber, M. C. 1998. Stearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome. Biochimica et Biophysica Acta 1391: 145–156.Google Scholar
Wise, L. S. and Green, H. 1979. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. Journal of Biological Chemistry 254: 273–275.Google Scholar
Wu, P., Sato, K., Suzuta, F., Hikasa, Y. and Kagota, A. 2000. Effects of lipid-related factors on adipocyte differentiation of bovine stromal-vascular cells in primary culture. Journal of Veterinary Medical Science 62: 933–939.CrossRefGoogle ScholarPubMed