Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-10T13:28:13.176Z Has data issue: false hasContentIssue false
  • English
  • Français

Diurnal variation in concentrations of various markers of bone metabolism in growing female goats and sheep

Published online by Cambridge University Press:  18 August 2016

A. Liesegang ,
M.-L. Sassi  and
J. Risteli
A. Liesegang*
Affiliation:
Institute of Animal Nutrition, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland
M.-L. Sassi
Affiliation:
Department of Clinical Chemistry, University of Oulu, FIN-90220 Oulu, Finland
J. Risteli
Affiliation:
Department of Clinical Chemistry, University of Oulu, FIN-90220 Oulu, Finland
*
† E-mail: aKese@vetphys.unizh.ch
Get access

Abstract

Twelve 6-month-old growing female goats and sheep were used in this study. Blood samples were obtained in the morning before goats and sheep were given food and then at 2-h intervals for 24 h (part I). This procedure was repeated 2 weeks later (part II). Concentrations of osteocalcin (OC), activities of total (tAP) and bone-specific alkaline phosphatase (bAP), degradation products of C-terminal telopeptide of type-I collagen (CrossLaps™ CL), and carboxyterminal telopeptide of type-I collagen (ICTP) were measured in serum.

In both parts of the study, all bone marker concentrations were significantly higher in goats than in sheep. The OC concentrations in goats increased in the late afternoon/evening and decreased thereafter to reach values similar to those obtained at the beginning. The ICTP concentrations in goats slowly decreased until 14:00 h, increased, and decreased again. The concentrations in sheep decreased continuously but not significantly, towards the morning sampling. The CL concentrations increased in both sheep and goats during the night but at 06:00 h started to decrease to levels found at the beginning of testing. The bAP activities decreased in goats from 20:00 to 22:00 h. Changes in the concentrations of bone markers were mainly observed in goats of this study. As documented for bone resorption and formation in other species, circadian rhythms were evident for concentrations of ICTP, CL, bAP and OC. The present study indicates that growing goats may have a physiologically higher bone turn-over than growing sheep, because the bone marker concentrations were always higher.

Keywords

biochemical markersbone formationdiurnal variationgoatssheep
Type
Growth, development and meat science
Information
Animal Science , Volume 77 , Issue 2 , October 2003 , pp. 197 - 203
Copyright
Copyright © British Society of Animal Science 2003

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allen, M. J., Hofmann, W. E., Richardson, D. C. and Breur, G. J. 1998. Serum markers of bone metabolism in dogs. American Journal of Veterinary Research 59: 250–254.Google Scholar
Aoshima, H., Kushida, K., Takahashi, M., Ohishi, T., Hoshino, H., Suziki, M. and Inoue, T. 1998. Circadian variation of urinary type I collagen crosslinked C-telopeptide and free and peptide-bound forms of pyridinium crosslinks. Bone 22: 73–78.Google Scholar
Assad, M., Chernyshov, A. V., Jarzem, P., Leroux, M. A., Coillard, C., Charette, S. and Rivard, C. H. 2003a. Porous titanium-nickel for intervertebral fusion in a sheep model 1. Histomorphometric and radiological analysis. Journal of Biomedical Materials Research 64B: 107–120.CrossRefGoogle Scholar
Assad, M., Chernyshov, A. V., Jarzem, P., Leroux, M. A., Coillard, C., Charette, S. and Rivard, C. H. 2003b. Porous titanium-nickel for intervertebral fusion in a sheep model. 2. Surface analysis and nickel release assessment. Journal of Biomedical Materials Research 64B: 121–129.CrossRefGoogle Scholar
Cahoon, S., Boden, S.D., Gould, K. G. and Vailas, A. C. 1996. Noninvasive markers of bone metabolism in the rhesus monkey: normal effects of age and gender. Journal of Medical Primatology 25: 333–338.Google Scholar
Charles, P., Mosekilde, L., Risteli, L., Risteli, J. and Eriksen, E. F. 1994. Assessment of bone remodeling using biochemical indicators of type I collagen synthesis and degradation: relation to calcium kinetics. Bone and Mineral 24: 81–94.CrossRefGoogle ScholarPubMed
Chavassieux, P., Buffet, A., Vergnaud, P., Garnero, P. and Meunier, P. J. 1997. Short-term effects of corticosteroids on trabecular bone remodelling in old ewes. Bone 20: 452–455.Google Scholar
Chavassieux, P., Garnero, P., Duboef, F., Vergnaus, P., Brunner-Ferber, F., Delmas, P. D. and Meunier, P. J. 2001. Effect of new selective estrogen receptor modulator (MDL 103, 323) on cancellous and cortical bone in ovariectomized ewes: a biochemical, histomorphometric and densitometric study. Journal of Bone and Mineral Research 16: 89–96.Google Scholar
Chavassieux, P., Pastoureau, P., Boivin, G., Delmas, P. D., Chapuy, M. C. and Meunier, P. J. 1991. Effects of ossein-hydroxyapatite compound on ewe bone remodelling: biochemical and hisotmorphometric study. Clinical Rheumatology 10: 269–273.Google Scholar
Chavassieux, P., Pastoureau, P., Chapuy, M. C., Delmas, P. D. and Meunier, P. J. 1993. Glucosteroid-induced inhibition of osteoblastic bone formation in ewes: a biochemical and histomorphometric study. Osteoporosis International 3: 97–102.Google Scholar
Christenson, R. H. 1997. Biochemical markers of bone metabolism: an overview. Clinical Biochemistry 30: 573–593.CrossRefGoogle ScholarPubMed
Corlett, S. C., Couch, M., Care, A. D. and Sykes, A. R. 1990. Measurement of plasma osteocalcin in sheep: assessment of circadian variation, the effects of age and nutritional status and the response to perturbation of the adrenocortical axis. Experimental Physiology 75: 515–527.Google Scholar
Demers, L. M. 2001. Bone-specific alkaline phosphatase. In Bone markers, first edition (ed. Eastell, R., Baumann, M., Hoyle, N.R. and Wiezorek, L.), pp. 57–59. Martin Dunitz Ltd, London.Google Scholar
Egger, C. D., Mühlebauer, R. C., Felix, R., Delmas, P. D., Marks, S. C. and Fleisch, H. 1994. Evaluation of urinary pyridinium crosslink excretion as a marker of bone resorption in the rat. Journal of Bone and Mineral Research 9: 1211–1219.CrossRefGoogle ScholarPubMed
Eriksen, E. F., Charles, P., Melsen, F., Mosekilde, L., Risteli, L. and Risteli, J. 1993. Serum markers of type I collagen formation and degradation in metabolic bone disease. Correlation to bone histomorphometry. Journal of Bone and Mineral Research 8: 127–132.Google Scholar
Eyre, D. R., Paz, M. A. and Gallop, P. M. 1996. Crosslinking in collagen and elastin. Annual Review of Biochemistry 53: 717–748.Google Scholar
Geor, R., Hope, E., Lauper, L., Piela, S., Klassen, J., King, V. and Murphy, M. 1995. Effect of glucocorticoids on serum osteocalcin concentration in horses. American Journal of Veterinary Research 56: 1201–1205.CrossRefGoogle ScholarPubMed
Gundberg, C. M., Makkowitz, M. E., Mizruchi, M. and Rosen, M. F. 1985. Osteocalcin in human serum: a circadian rhythm. Journal of Clinical Endocrinology and Metabolism 60: 736–739.Google Scholar
Hassager, C., Risteli, J., Risteli, L., Jensen, S. B. and Christiansen, C. 1992. Diurnal variation in serum markers of type I collagen synthesis and degradation in healthy premenopausal women. Journal of Bone and Mineral Research 7: 1307–1311.CrossRefGoogle ScholarPubMed
Huq, N. L., Teh, L. C., Christie, D. L. and Chapman, G. E. 1984. The amino acid sequences of goat, pig and wallaby osteocalcins. Biochemical International 8: 521–527.Google Scholar
Keijser, L. C. M., Schreuder, W. B., Boons, H. W., Keulers, B. J., Buma, P., Huiskes, R. and Veth, R. P. H. 2002. Bone grafting of cryosurgically treated bone defects: experiments in goats. Clinical Orthopaedics and Related Research 396: 215–222.Google Scholar
Leary, E. T. 2001. C-Telopetids. In Bone markers, first edition (ed. R. Eastell, , Baumann, M., Hoyle, N.R. and Wiezorek, L.), pp. 39–48. Martin Dunitz Ltd, London.Google Scholar
Lepage, O. M., Des Coteaux, L., Marcoux, M. and Tremblay, A. 1991. Circadian rhythms of osteocalcin in equine serum. Correlation with alkaline phosphatase, calcium, phosphate and total protein levels. Canadian Journal of Veterinary Research 55: 5–10.Google ScholarPubMed
Lepage, O. M., Marcoux, M. and Tremblay, A. 1990. Serum osteocalcin or bone gla-protein, a biochemical marker for bone metabolism in horses: differences in serum levels with age. American Journal of Veterinary Research 54: 223–226.Google Scholar
Liesegang, A., Reutter, R., Sassi, M.-L., Risteli, J., Kraenzlin, M., Riond, J.-L. and Wanner, M. 1999. Diurnal variation in concentration of various markers of bone metabolism in beagle dogs. American Journal of Veterinary Research 60: 949–953.Google Scholar
Liesegang, A., Sassi, M.-L., Risteli, J., Eicher, R., Wanner, M. and Riond, J.-L. 1998. Comparison of bone resorption markers during hypocalcemia in dairy cows. Journal of Dairy Science 81: 2614–2622.Google Scholar
Mende, L. M., Huq, N. L., Matthews, H. R. and Chapman, G. E. 1984. Primary structure of osteocalcin from ovine bone. DABITC sequencing of 4-hydroxyproline and gamma-carboxyglutamate residues. International Journal of Peptide and Protein Research 24: 297–302.Google Scholar
Miller, S. C., Bowman, B. M. and Jee, W. S. S. 1995. Available animal models of osteopenia – small and large. Bone 17: 117S–123S.Google Scholar
Moroni, A. M., Faldini, C., Giannini, S. and Wippermann, B. 2003. Plate fixation with hydroxyapatite-coated screws: a comparative loaded study. Clinical Orthopaedics and Related Research 408: 262–267.Google Scholar
Mühlbauer, R. C. and Fleisch, H. 1995. The diurnal rhythm of bone resorption in the rat. Journal of Clinical Investigation 95: 1933–1940.Google Scholar
Newman, E., Turner, A. S. and Wark, J. D. 1995. The potential of sheep for the study of osteopenia: current status and comparison with other animal models. Bone 16: 277S–284S.Google Scholar
Nielsen, H. K., Charles, P. and Mosekilde, L. 1988. The effect of single oral doses of prednisone on the circadian rhythm of serum osteocalcin in normal subjects. Journal of Clinical Endocrinology and Metabolism 65: 290–294.Google Scholar
O’Connell, S.L., Tresham, J., Fortune, C. L., Farrugia, W., McDougall, J. G., Scoggins, B. A. and Wark, J. D. 1993. Effects of prednisolone and deflazacort on osteocalcin metabolism in sheep. Calcified Tissue International 53: 117–121.CrossRefGoogle ScholarPubMed
Pietschmann, P., Resch, H., Woloszczuk, W. and Willvonseder, R. 1990. A circadian rhythm of serum osteocalcin levels in postmenopausal osteoporosis. European Journal of Clinical Investigation 20: 310–312.Google Scholar
Qvist, P., Christgau, S., Pedersen, B. J., Schlemmer, A. and Christiansen, C. 2002. Circadian variation in the serum concentration of C-terminal telopeptide of type I collagen (serum CTx): effects of gender, age, menopausal status, posture, daylight, serum cortisol, and fasting. Bone 31: 57–61.CrossRefGoogle ScholarPubMed
Risteli, L. and Risteli, J. 1993. Biochemical markers of bone metabolism. Annals of Medicine 25: 385–393.Google Scholar
Risteli, L. and Risteli, J. 1997. Assays of type I procollagen domains and collagen fragments: problems to be solved and future trends. Scandinavian Journal of Clinical and Laboratory Investigation 57: 105–113.Google Scholar
Saggese, G., Baroncelli, G. I., Bertelloni, S., Cinquanta, L. and DiNero, G. 1994. Twenty-four-hour osteocalcin, carboxyterminal propeptide of type I, and aminoterminal propeptide of type III procollagen rhythms in normal and growth-retarded children. Pediatric Research 35: 409–415.Google Scholar
Sassi, M. L., Eriksen, H., Risteli, L., Niemi, S., Mansell, J., Gowen, M. and Risteli, J. 2000. Immunochemical characterization of assay for carboxyterminal telopeptide of human type I collagen: loss of antigenicity by treatment with cathepsin K. Bone 26: 367–373.CrossRefGoogle ScholarPubMed
Schlemmer, A., Hassager, C., Jensen, S. B. and Christiansen, C. 1992. Marked diurnal variation in urinary excretion of pyridinium cross-links in premenopausal women. Journal of Clinical Endocrinology and Metabolism 74: 476–480.Google ScholarPubMed
Scott, D., Abu Damir, H., Buchan, W., Duncan, A. and Robins, S. P. 1993. Factors effecting urinary pyridinoline and deoxypyridinoline excretion in the growing lamb. Bone 14: 807–811.Google Scholar
Smith, M. C. and Sherman, D. M. 1994. Goat medicine. Lea and Febiger, Philadelphia, PA.Google Scholar
Srivatava, A. K., Bhattacharyya, S., Li, X., Mohan, S. and Baylink, D. J. 2001. Circadian and longitudinal variation of serum C-telopeptide, osteocalcin, and skeletal alkaline phosphatase. Bone 29: 361–367.CrossRefGoogle Scholar
Statistical Packages for the Social Sciences. 2000. Systat version 8. SPSS Inc., Chicago, IL.Google Scholar
Syakalima, M., Takiguchi, M., Yasuda, J. and Hashimoto, A. 1997. Separation and quantification of corticosteroid-induced, bone and liver alkaline phosphatase isoenzymes in canine serum. Journal of Veterinary Medicine A 44: 603–610.Google Scholar
Turner, A. S. 2002. The sheep as a model for osteoporosis in humans. The Veterinary Journal 163: 232–239.Google Scholar
Turner, A. S., Alvis, M., Myers, W., Stevens, M. L. and Lundy, M. W. 1995. Changes in bone mineral density and bone-specific alkaline phosphatase in ovariectomized ewes. Bone 17: 395S–402S.Google Scholar
Wuisman, P. J., Dijk, M. van Smit, T. H. 2002. Resorbable cages for spinal fusion: an experimental goat model. Orthopedics 25: 1141–1148.CrossRefGoogle ScholarPubMed