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Effect of extensive feeding on α-tocopherol concentration and oxidative stability of muscle microsomes from Iberian pigs

Published online by Cambridge University Press:  02 September 2010

A. I. Rey ,
C. J. Lopez-Bote  and
R. Sanz Arias
A. I. Rey
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
C. J. Lopez-Bote
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
R. Sanz Arias
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
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Abstract

The effect of extensive feeding, confinement and diet supplementation with a-tocopheryl acetate (100 mg/kg) on the fatty acid composition and tocopherol concentration of microsome extracts and their susceptibility to oxidation was studied in Iberian pigs. The diet of pigs raised extensively was mostly composed of acorn and grass. The a-tocopherol contents of acorn and grass were 20 and 171 mg/kg dry matter, respectively. Microsomal fatty acid composition showed no differences among groups. Pigs feeding extensively had a higher concentration of a-tocopherol in muscle and microsomes than pigs given mixed diet with the basal level of a-tocopheryl acetate (P < 0·05) but lower values than pigs given supplementary levels (100 mg/kg) (P < 0·05). Microsomal fractions from pigs given mixed diet with a basal level of a-tocopheryl acetate were significantly more susceptible to iron-induced lipid oxidation than extract from pigs given diets containing a supplementary level (P < 0·05). Microsomal extracts from pigs feeding extensively had the lowest oxidation rate (P < 0·05), suggesting that other dietary constituents may play a role in the stabilization of microsomal lipids.

Keywords

extensive husbandrymicrosomesoxidationpigsα-tocopherol
Type
Research Article
Information
Animal Science , Volume 65 , Issue 3 , December 1997 , pp. 515 - 520
Copyright
Copyright © British Society of Animal Science 1997

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References

Addis, P. B. and Park, S. W. 1989. Role of lipid oxidation products in atherosclerosis. In Food toxicology (ed. Scanlan, R. and Taylor, S. L.), pp. 297–330. Marcel Dekker Inc., New York.Google Scholar
Allen, C. E. and Foegeding, E. A. 1981. Some lipid characteristics and interactions in muscle foods — a review. Food Technology 35: 253–257.Google Scholar
Antequera, T., Lopez-Bote, C. J., Cordoba, J. J., Garcia, C., Asensio, M. A. and Ventanas, J. 1992. Lipid oxidative changes in the processing of the Iberian pig hams. Food Chemistry 45:105–110.CrossRefGoogle Scholar
Aparicio, J. B. 1987. El cerdo Iberico. Premio de investigation. Sanchez Romero Carvajal, Jabugo, Huelva, Spain.Google Scholar
Ashgar, A., Lin, C. F., Gray, J. I., Buckley, D. J., Booren, A. M., Crackel, R. L. and Flegal, C. J. 1989. The influence of oxidised dietary oil and antioxidant supplementation on membrane-bound lipid stability in broiler meat. British Poultry Science 30: 817–825.Google Scholar
Association of Official Analytical Chemists. 1984. Official methods of analysis (ed. Williams, S.). Association of Official Analytical Chemists, Arlington, VA.Google Scholar
Bligh, E. G. and Dyer, W. J. 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917.CrossRefGoogle ScholarPubMed
Brown, F. 1953. The tocopherol content of farm feedstuffs. Journal of the Science ofFood and Agriculture 4:161–165.CrossRefGoogle Scholar
Burgstaller, G. and Jatsch, C. 1991. Fattening of heavy pigs, up to 160 kg liveweight, for the production of long-life sausage based on home-produced forage. 2. Influence of grass meal on fatty acid pattern of mesenteric fat and backfat. Wirtschaftseigene-Futter 37:308–317.Google Scholar
Cava, R., Ruiz, J., Lopez-Bote, C. J., Martin, L., Garcia, C., Ventanas, J. and Antequera, T. 1997. Influence of finishing diet on intramuscular lipids, triglycerides and phospholipid fatty acid profiles in muscle of Iberian pig. Meat Science 45: 263–270.Google Scholar
Elmadfa, I. 1995. Physiological importance of unsaponifiable components in dietary fat. Felt Wissenschaft Technologic 97: 85–90.Google Scholar
Engeseth, N. J. 1990. Membranal muscle oxidation in muscle tissue — mechanism and prevention. Ph.D. thesis, Michigan State University.Google Scholar
Garcia, C., Berdague, J. L., Antequera, T., Lopez-Bote, C. J., Cordoba, J. J. and Ventanas, J. 1991. Volatile components of dry cured Iberian ham. Food Chemistry 41: 23–32.CrossRefGoogle Scholar
Gardner, H. W. 1979. Lipid hydroperoxide reactivity with proteins and amino acids. A review. Journal of Agricultural and Food Chemistry 27: 220–229.CrossRefGoogle Scholar
Gray, J. I. and Pearson, A. M. 1987. Rancidity and warmed-over flavor. In Advances in meat research (ed. Pearson, A. M. and Dutson, T. R.) pp. 221–270. Van Nostrand Reinhold Company, New York.Google Scholar
Hakkarainen, J. and Pehrson, B. 1987. Vitamin E and polyunsaturated fatty acids in Swedish feedstuffs for cattle. Ada Agriculturae Scandinavka 37:341–346.Google Scholar
Harel, S. and Kanner, J. 1985. Muscle membranal lipid peroxidation initiated by hydrogen peroxide-activated metmyoglobin. Journal of Agricultural and Food Chemistry 33:1188–1192.Google Scholar
Kumaravelu, P., Subramaniyam, S., Dakshinamoorthy, D. P. and Devaraj, N. S. 1996. The antioxidant effect of eugenol on cc14-induced erythrocyte damage in rats. Journal ofNutritional Biochemistry 7: 23–28.Google Scholar
Lin, C. F., Gray, J. I., Ashgar, A., Buckley, D. J., Booren, A. M. and Flegal, C. J. 1989. Effect of dietary oils and a-tocopherol supplementation on lipid peroxidation in broiler meat. Journal of Food Science 54:1457–1460.Google Scholar
Lopez-Bote, C., Rey, A., Ruiz, J.Isabel, B. and Sanz Arias, R. 1997. Effect of feeding diets high in monounsaturated fatty acids and a-tocopheryl acetate to rabbits on resulting carcass fatty acid profile and lipid oxidation. Animal Science 64:177–186.CrossRefGoogle Scholar
Lopez-Bote, C. J., Gomaa, E., Gray, J. I. and Flegal, C. J. 1992. Stabilization of broiler lipids (including cholesterol) through dietary supplementation with spice extracts. Proceedings of the 37th international congress of meat and technology, pp. 523–525.Google Scholar
Lynch, G. L. 1991. Natural occurrence and content of vitamin E in feedstuffs. In Vitamin E in animal nutrition and management (ed. Coelho, M. B.), pp 43–48. BASF corporation, Parsippany, NJ.Google Scholar
Martin, L. 1996. Influencia de las condiciones del procesado sobre los cambios madurativos en el jamon iberico. Ph.D. thesis, Universidad de Extremadura, Caceres, Spain.Google Scholar
Monahan, F. J., Buckley, D. J., Morrissey, P. A., Lynch, P. B. and Gray, J. I. 1992. Influence of dietary fat and α-tocopherol supplementation on lipid oxidation in pork. Meat Science 31: 229–241.Google Scholar
Monahan, F. J., Gray, J. I., Ashgar, A., Haug, A., Strasburg, G. M., Buckley, D. J. and Morrissey, P. A. 1994. Influence of diet on lipid oxidation and membrane structure in porcine muscle microsomes. Journal of Agricultural and Food Chemistry 42: 59–63.CrossRefGoogle Scholar
Mutetikka, D. B. and Mahan, D. C. 1993. Effect of pasture, confinement, and diet fortification with vitamin E and selenium on reproducing gilts and their progeny. Journal of Animal Science 71: 3211–3218.Google Scholar
National Research Council. 1985. Guide for the care and use of laboratory animals. Publication no. 85–23, NIH, Washington, DC.Google Scholar
National Research Council. 1988. Nutrient requirements of swine. National Academy Press, Washington, DC.Google Scholar
Rafii, Z. A., Zavarin, E. and Pelleau, Y. 1991. Chemosystematic differentiation of Quercus ilex and Quercus rotundifolia based on acorn fatty acids. Biochemical Systematics and Ecology 19:163–166.CrossRefGoogle Scholar
Reddy, A. C. P. and Lokesh, B. R. 1992. Studies on spice principles as antioxidants in the inhibition of lipid-peroxidation of rat liver microsomes. Molecular and Cellular Biochemistry 111: 117–124.Google Scholar
Rey, A., Lopez-Bote, C., Soares, M. and Isabel, B. 1996. Determination of α-tocopherol in pork with high intramuscular fat content. Grasas y Aceites 47: 331–334.Google Scholar
Rhee, K. S. 1988. Enzymic and non-enzymic catalysis of lipid oxidation in mucle foods. Food Technology 42:127–132.Google Scholar
Rice, D. A., Blanchflower, W. J. and McMurray, C. H. 1981. Reproduction of nutritional degenerative myopathy in the post ruminant calf. Vétérinary Record 109:161–162.Google Scholar
Sans, P. and Darre, R. 1993. Renewed interest for the old local swine Gascon breed. Revue de Medecine Vétérinaire 144: 343–347.Google Scholar
Sans, P., Darre, R. and Griess, D. 1994. Feeding efficiency and carcasses characteristics in Gascon pigs kept in an outdoors system. Revue de Médecine Vétérinaire 145: 867–871.Google Scholar
Statistical Analysis Systems Institute. 1988. SAS user's guide: statistics. SAS Institute Inc., Cary, NC.Google Scholar
Tramontano, W. A., Ganci, D., Pennino, M. and Dierenfeld, E. S. 1993. Distribution of α-tocopherol in early foliage samples in several forage crops. Phytochemistry 34: 389–390.Google Scholar
Ventanas, J., Cordoba, J. J., Antequera, T., Garcia, C., Lopez-Bote, C. J. and Asensio, M. A. 1992. Hydrolysis and Maillard reactions during ripening of Iberian ham. Journal of Food Science 57: 813–815.Google Scholar
Yamauchi, K., Nagal, Y., Yada, K., Ohashi, T. and Pearson, A. M. 1984. A relationship between a-tocopherol and polyunsaturated fatty acids in chicken and porcine skeletal muscle mitochondria. Agricultural and Biological Chemistry 11: 2827–2830.Google Scholar