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Effect of litter size on the milk production, blood metabolite profiles and endocrine status of ewes lambing in January and April

Published online by Cambridge University Press:  02 September 2010

S. M. Rhind ,
J. Bass ,
J. M. Doney  and
E. A. Hunter
S. M. Rhind
Affiliation:
Macaulay Land Use Research Institute, Pentlandfield, Roslin, Midlothian EH25 9RF
J. Bass
Affiliation:
Macaulay Land Use Research Institute, Pentlandfield, Roslin, Midlothian EH25 9RF
J. M. Doney
Affiliation:
Macaulay Land Use Research Institute, Pentlandfield, Roslin, Midlothian EH25 9RF
E. A. Hunter
Affiliation:
Scottish Agricultural Statistical Services, Kings Buildings, Mayfield Road, Edinburgh
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Abstract

Milk yields and blood metabolite and hormone profiles of a total of 40 Greyface ewes lambing in January or April and suckling single (S) or twin (T) lambs were determined. Ewes were given a fixed level of feeding throughout the first 10 weeks of lactation. Milk yields were measured weekly. Pooled blood samples (six samples; 20-min intervals), collected at weekly intervals for the first 10 weeks of lactation, were assayed for glucose, non-esterified fatty acids (NEVA), 3β-hydroxybutyrate, urea, albumin, total protein, insulin, growth hormone (GH), cortisol, prolactin, thyroxine (T4) and triiodothyronine (T3). At weeks 2, 4 and 10 of lactation samples were collected at 20-min intervals for 8 h and assayed individually for insulin, GH, cortisol and prolactin. Mean daily milk yield was lower in S than in T ewes in January (1·93 v. 2·33 kg/day; P < 0·05) and April-lambing ewes (1·95 v. 2·28 kg/day; P > 0·05). Profiles of NEFA and 3β-hydroxybutyrate indicated that T ewes were mobilizing adipose tissue at a greater rate than S ewes. The higher milk yield and rate of fat mobilization in T ewes compared with S ewes was associated with lower overall mean insulin concentrations (3·37 v. 5·04 mil per I; P < 0·002) and higher GH (6·29 v. 2·77 μg/l; P < 0·001) and cortisol (7·04 v. 3·64 μg/l; P < 0·001) concentrations in the weekly samples. Differences between rearing groups in mean concentrations of T4 and T3 were generally small and not significant. Mean prolactin concentrations were much lower in the January- than in the April-lambing ewes (73·7 v. 270·2 μ/l; P < 0·001) but this difference was not associated with a difference in milk yield. Substantial increases in insulin and GH concentrations generally followed feeding but the post-prandial profiles were dependent on stage of lactation; while GH concentrations increased following feeding at weeks 2 and 4 of lactation, there was generally a decline in post-prandial concentrations at week 10. It is concluded that the patterns of insulin and GH secretion during the hours following feeding may be an important determinant of the rate of tissue mobilization and milk yield in ewes subject to differences in demand for milk created by differences in litter sizes.

Keywords

blood compositioneweshormone secretionlitter sizemilk production
Type
Research Article
Information
Animal Production , Volume 53 , Issue 1 , August 1991 , pp. 71 - 80
Copyright
Copyright © British Society of Animal Science 1991

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References

Bassett, J. M. 1974a. Early changes in plasma insulin and growth hormone levels after feeding in lambs and adult sheep. Australian journal of Biological Sciences 27: 157–166.CrossRefGoogle ScholarPubMed
Bassett, J. M. 1974b. Diurnal patterns of plasma insulin, growth hormone, corticosteroid and metabolite concentrations in fed and fasted sheep. Australian journal of Biological Sciences 27: 167–181.CrossRefGoogle ScholarPubMed
Bassett, J. M. 1978. Endocrine factors in the control of nutrient utilisation: ruminants. Proceedings of the Nutrition Society 37: 273–280.CrossRefGoogle ScholarPubMed
Bines, J. A., Hart, I. C. and Morant, S. V. 1983. Endocrine control of energy metabolism in the cow: diurnal variations in the concentrations of hormones and metabolites in the blood plasma of beef and dairy cows. Hormone and Metabolic Research 15: 330–334.CrossRefGoogle ScholarPubMed
Blaxter, K. L., Reineke, E. P., Crampton, E. W. and Petersen, W. E. 1949. The role of thyroidal materials and of synthetic goitrogens in animal production and an appraisal of their practical use. Journal of Animal Science 8: 307–352.CrossRefGoogle Scholar
Doney, J. M., Peart, J. N., Smith, W. F. and Louda, F. 1979. A consideration of the techniques for estimation of milk yield by suckled sheep and a comparison of estimates obtained by two methods in relation to the effect of breed, level of production and stage of lactation. Journal of Agricultural Science, Cambridge 92:123–132.CrossRefGoogle Scholar
Failing, J. F., Buckley, M. W. and Zak, B. 1960. Automatic determination of serum proteins. American journal of Clinical Pathology 33: 83–88.CrossRefGoogle Scholar
Gardner, R. W. and Hogue, D. E. 1964. Effects of energy intake and number of lambs suckled on milk yield, milk composition and energetic efficiency of lactating ewes. journal of Animal Science 23: 935–942.CrossRefGoogle Scholar
Hart, I. C., Bines, J. A., Morant, S. V. and Ridley, J. L. 1978. Endocrine control of energy metabolism in the cow: comparison of the levels of hormones (prolactin, growth hormone, insulin and thyroxine) and metabolites in the plasma of high- and low-yielding cattle at various stages of lactation. Journal of Endocrinology 77: 333–345.CrossRefGoogle ScholarPubMed
Hart, I. C., Flux, D. S., Andrews, P. and McNeilly, A. S. 1975. Radioimmunoassay for ovine and caprine growth hormones: its application to the measurement of basal circulating levels of growth hormone in the goat. Hormone and Metabolic Research 7: 35–40.CrossRefGoogle Scholar
McCance, I. 1959. The determination of milk yield in the Merino ewe. Australian Journal of Agricultural Research 10: 839–853.CrossRefGoogle Scholar
McNeilly, A. S. and Andrews, P. 1974. Purification and characterization of caprine prolactin. Journal of Endocrinology 60: 359–367.CrossRefGoogle ScholarPubMed
Paterson, D. S. P. 1963. Some observations on the estimation of non-esterified fatty acid concentrations in cow and sheep plasma. Research in Veterinary Science 4: 230–237.CrossRefGoogle Scholar
Peart, J. N. 1982. Lactation of suckling ewes and does. In Sheep and goat production (ed. Coop, I. E.), World Animal Science Series Volume 26, pp. 119–133. Elsevier, Amsterdam.Google Scholar
Peart, J. N., Edwards, R. A. and Donaldson, E. 1972. The yield and composition of the milk of Finnish Landrace × Blackface ewes. I. Ewes and lambs maintained indoors. Journal of Agricultural Science, Cambridge 79: 303–313.CrossRefGoogle Scholar
Richardson, T. 1977. A modification of the Trinder Auto Analyzer method for glucose. Annals of Clinical Biochemistry 14: 223–226.CrossRefGoogle Scholar
Russel, A. J. F., Doney, J. M. and Gunn, R. G. 1969. Subjective assessment of body fat in live sheep. Journal of Agricultural Science, Cambridge 72:451–454.CrossRefGoogle Scholar
Salacinski, P. R. P., McLean, C., Sykes, J. E. C., Clement-Jones, V. V. and Lowry, P. J. 1981. Iodination of proteins, glyocoproteins, and peptides using a solid-phase oxidizing agent, 1, 3, 4, 6, tetrachloro-3α, 6α, diphenyl glycoluril (Iodogen). Analytical Biochemistry 117:136–146.CrossRefGoogle Scholar
Spencer, K. and Price, C. P. 1977. Influence of reagent quality and reaction conditions on the determination of serum albumin by the bromocresol green dye-binding method. Annals of Clinical Biochemistry 14:105–115.CrossRefGoogle Scholar
Tindal, J. S., Knaggs, G. S., Hart, I. C. and Blake, L. A. 1978. Release of growth hormone in lactating and nonlactating goats in relation to behaviour, stages of sleep, electro-encephalograms, environmental stimuli and levels of prolactin, insulin, glucose and free fatty acids in the circulation. Journal of Endocrinology 76: 333–346.CrossRefGoogle Scholar
Trenkle, A. 1971. Effect of diet upon levels of plasma growth hormone in sheep. Journal of Animal Science 32: 111–114.CrossRefGoogle ScholarPubMed
Trenkle, A. 1978. Relation of hormonal variations to nutritional studies and metabolism of ruminants. Journal of Dairy Science 61: 281–293.CrossRefGoogle ScholarPubMed
Trenkle, A. 1981. Endocrine regulation of energy metabolism in ruminants. Federation Proceedings 40: 2536–2541.Google ScholarPubMed
Walsh, D. S., Vesely, J. A. and Mahadevans, S. 1980. Relationship between milk production and circulating hormones in dairy cows. Journal of Dairy Science 63: 290–294.CrossRefGoogle Scholar
Wilcox, A. A., Carroll, W. E., Sterling, R. E., Davis, H. A. and Ware, A. G. 1966. Use of the Berthelot reaction in automated analysis of serum urea nitrogen. Clinical Chemistry 12: 151–157.CrossRefGoogle ScholarPubMed
Wilde, C. J., Calvert, D. T., Daly, A. and Peaker, M. 1987. The effect of goat milk fractions on synthesis of milk constituents by rabbit mammary explants and on milk yield in vivo. Biochemical Journal 242: 285–288.CrossRefGoogle ScholarPubMed
Zivin, J. A. and Snarr, J. F. 1973. An automated colorimetric method for the measurement of 3-hydroxybutyrate concentration. Analytical Biochemistry 52: 456–461.CrossRefGoogle ScholarPubMed