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Effect of nucleotide supplementation on lymphocyte DNA damage induced by dietary oxidative stress in pigs

Published online by Cambridge University Press:  09 March 2007

J. Salobir ,
V. Rezar ,
T. Pajk  and
A. Levart
J. Salobir*
Affiliation:
Institute of Nutrition, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia
V. Rezar
Affiliation:
Institute of Nutrition, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia
T. Pajk
Affiliation:
Institute of Nutrition, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia
A. Levart
Affiliation:
Institute of Nutrition, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia
*
†E-mail: janez.salobir@bfro.uni-lj.si
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Abstract

The aim of the present study was to evaluate the effect of nucleotide supplementation on the oxidative stress induced by a high proportion of dietary polyunsaturated fatty acids ( PUFAs) in pigs. Twenty-four male growing pigs were penned individually and after an adaptation period divided into three groups. All groups received isocaloric daily rations composed of a basal diet supplemented with either: starch (CONT), linseed oil (LIN) and LIN and nucleotides (LIN + NUC). The experimental period lasted 21 days. Oxidative stress was evaluated by measuring the degree of lymphocyte nuclear DNA damage, the urine malondialdehyde ( MDA) excretion rate, erythrocyte glutathione peroxidase concentration and the total anti-oxidant status of plasma. Malondialdehyde concentrations in the blood and MDA urinary excretion rates were higher (P < 0·01) in animals supplemented with LIN and LIN + NUC compared with CONT animals. The degree of DNA damage in the LIN-supplemented animals was also higher (P < 0·01). Compared with the LIN-supplemented animals, nucleotide supplementation reduced (P < 0·01) the degree of DNA damage in lymphocytes to the level of the CONT group. Erythrocyte glutathione peroxidase concentration and plasma total anti-oxidant status were similar across treatments. The results of this experiment indicate that nucleotide supplementation effectively eliminates the genotoxic effects of high PUFA intakes on blood lymphocytes and demonstrates new evidence for the immunonutritive effect of nucleotides.

Keywords

damagemalondialdehydenucleotidesoxidative stress
Type
Research Article
Information
Animal Science , Volume 81 , Issue 1 , August 2005 , pp. 135 - 140
Copyright
Copyright © British Society of Animal Science 2005

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