Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-28T02:56:24.938Z Has data issue: false hasContentIssue false
  • English
  • Français

Effectiveness of exogenous microbial phytase in improving the bioavailabilities of phosphorus and other nutrients in maize-soya-bean meal diets for broilers

Published online by Cambridge University Press:  18 August 2016

B. J. Camden ,
P.C.H. Morel ,
D. V. Thomas ,
V. Ravindran  and
M. R. Bedford
B. J. Camden
Affiliation:
Monogastric Research Centre, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
P.C.H. Morel
Affiliation:
Monogastric Research Centre, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
D. V. Thomas
Affiliation:
Monogastric Research Centre, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
V. Ravindran*
Affiliation:
Monogastric Research Centre, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
M. R. Bedford
Affiliation:
Finnfeeds International Ltd, Marlborough, Wiltshire SN8 1XN, UK
*
†Corresponding author. E-mail:V.Ravindran@massey.ac.nz
Get access

Abstract

A 3-week feeding trial using 300 1-day-old male broiler chicks was conducted to evaluate the effects of adding three levels of a microbial phytase (Finnfeed phytase; 250, 500 and 1000 U per kg diet) to a maize-soya-bean-meal diet containing 3·0 g/kg non-phytate phosphorus and 8·0 g/kg calcium. A diet containing 4·0 g/kg non-phytate phosphorus and 9·0 g/kg calcium served as the positive control. The responses were evaluated in terms of broiler performance, toe ash contents, ileal phytate degradation, ileal digestibility of nitrogen, amino acids, phosphorus, starch and fat, apparent metabolizable energy, apparent ileal digestible energy, and apparent retention of phosphorus and nitrogen. The addition of 500 U phytase per kg diet to the phosphorus-deficient maize-soya-bean-meal diet improved the performance and toe ash contents of broilers to equal those given the positive control diet. Ileal phytate degradation data provided direct evidence to the efficacy of phytase in hydrolysing the phytic acid. Addition of 500 U phytase per kg to the maize-soya-bean-meal diet which contained 3·0 g phytate-phosphorus per kg resulted in more than doubling of phytate degradation from 0·218 to 0·481. Supplemental phytase improved ileal digestibility of nitrogen, amino acids, starch and lipids, with these improvements being eventually reflected in enhancements in ileal digestible energy and apparent metabolizable energy.

Keywords

broilersnutrition physiologyperformancephytase
Type
Non-ruminant, nutrition, behaviour and production
Information
Animal Science , Volume 73 , Issue 2 , October 2001 , pp. 289 - 297
Copyright
Copyright © British Society of Animal Science 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Association of Official Analytical Chemists. 1990. Official methods of analysis. AOAC, Arlington, VA.Google Scholar
Atteh, J. O. and Leeson, S. 1984. Effects of dietary saturated or unsaturated fatty acids and calcium levels on performance and mineral metabolism in broilers. Poultry Science 63: 2252–2260.CrossRefGoogle ScholarPubMed
Caldwell, R. A. 1992. Effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin. Journal of Agricultural and Food Chemistry 40: 43–47.CrossRefGoogle Scholar
Cosgrove, D. J. 1966. The chemistry and biochemistry of inositol polyphosphates. Reviews in Pure and Applied Chemistry 16: 209–224.Google Scholar
Costigan, P. and Ellis, K. J. 1987. Analysis of faecal chromium derived from controlled release marker devices. New Zealand Journal of Technology 3: 89–92.Google Scholar
Klis, J. D. van der and Versteegh, H. A. J. 1991. Ileal absorption of phosphorus in lightweight white laying hens using microbial phytase and various calcium contents in laying hen feed. Spelderholt publication no. 563, Spelderholt Research Institute, Beekbergen, The Netherlands.Google Scholar
Ledoux, D. R., Firman, J. D., Broomhead, J. N. and Li, Y. C. 1999. Effects of microbial phytase on apparent ileal digestibility of amino acids in turkey poults fed a corn- soybean meal diet formulated on an ideal protein basis. Poultry Science 78: (suppl. 1) 74 (abstr. ).Google Scholar
McCleary, B. V., Gibson, T. S. and Mugford, D. C. 1997. Measurement of total starch in cereal products by amyloglucosidase-a -amylase method: collaborative study. Journal of the Association of Official Analytical Chemists 80: 571–579.Google Scholar
Namkung, H. and Leeson, S. 1999. Effect of phytase enzyme on dietary nitrogen-corrected apparent metabolizable energy and the ileal digestibility of nitrogen and amino acids. Poultry Science 78: 1317–1319.CrossRefGoogle ScholarPubMed
National Research Council. 1994. Nutrient requirements of domestic animals. Nutrient requirements of poultry. National Academy Press, Washington, DC.Google Scholar
Nelson, T. S. 1967. The utilization of phytate phosphorus by the chick — a review. Poultry Science 46: 862–871.CrossRefGoogle Scholar
Potter, L. M. 1988. Bioavailability of phosphorus from various phosphates based on body weight and toe ash measurements. Poultry Science 67: 96–102.CrossRefGoogle ScholarPubMed
Ravindran, V., Bryden, W. L. and Kornegay, E. T. 1995. Phytates: occurrence, bioavailability and implications in poultry nutrition. Poultry and Avian Biology Reviews 6: 125–143.Google Scholar
Ravindran, V., Cabahug, S., Ravindran, G. and Bryden, W. L. 1999a. Influence of microbial phytase on apparent ileal amino acid digestibility in feedstuffs for broilers. Poultry Science 78: 699–706.CrossRefGoogle ScholarPubMed
Ravindran, V., Cabahug, S., Selle, P. H. and Bryden, W. L. 2000. Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non-phytate phosphorus levels. II. Effects on apparent metabolisable energy, nutrient digestibility and nutrient retention. British Poultry Science 41: 193–200.CrossRefGoogle ScholarPubMed
Ravindran, V., Hew, L. I., Ravindran, G. and Bryden, W. L. 1999b. A comparison of ileal digesta and excreta analysis for the determination of amino acid digestibility in food ingredients for poultry. British Poultry Science 40: 266–274.CrossRefGoogle ScholarPubMed
Schoner, V. F. J., Hoppe, P. P. and Schwarz, G. 1991. Comparative effects of microbial phytase and inorganic P on performance and on retention of P, Ca and crude ash in broilers. Journal of Animal Physiology and Animal Nutrition 66: 248–255.Google Scholar
Sebastian, S., Touchburn, S. P., Chavez, E. R. and Lague, P. C. 1997. Apparent digestibility of protein and amino acids in broiler chickens fed a corn-soybean diet supplemented with microbial phytase. Poultry Science 76: 1760–1769.CrossRefGoogle ScholarPubMed
Sharma, C. B., Goel, M. and Irshad, M. 1978. Myoinositol hexaphosphate as a potential inhibitor of a -amylases. Phytochemistry 17: 201–204.CrossRefGoogle Scholar
Simons, P. C. M., Versteegh, H. A. J., Jongbloed, A. W., Kemme, P. A., Slump, P., Bos, K. D., Wolters, M. G. E., Beudeker, R. F. and Verschoor, G. J. 1990. Improvement of phosphorus availability by microbial phytase in broilers and pigs. British Journal of Nutrition 64: 525–540.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute. 1997. SAS/STAT® user’s guide: statistics, version 6·12. SAS Institute Inc., Cary, NC.Google Scholar
Technicon. 1973. Industrial method no. 98/70w. Technicon, Tarrytown, NY.Google Scholar
Thompson, L. U. 1988. Antinutrients and blood glucose. Food Technology 36: 123–131.Google Scholar
Twine, J. R. and Williams, C. H. 1971. The determination of phosphorus in kjeldahl digests of plant material by automatic analysis. Communications in Soil Science and Plant Analysis 2: 485–489.CrossRefGoogle Scholar
Yi, Z., Kornegay, E. T. and Denbow, D. M. 1996a. Effect of microbial phytase on nitrogen and amino acid digestibility and nitrogen retention of turkey poults fed corn-soybean meal diets. Poultry Science 75: 979–990.CrossRefGoogle ScholarPubMed
Yi, Z., Kornegay, E. T., Ravindran, V. and Denbow, D. M. 1996b. Improving phytate phosphorus availability in corn and soybean meal for broilers using microbial phytase and calculation of phosphorus equivalency values for phytase. Poultry Science 75: 240–249.CrossRefGoogle ScholarPubMed