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The effects of an Aspergillus oryzae extract containing alpha-amylase activity on ruminal fermentation and milk production in lactating Holstein cows

Published online by Cambridge University Press:  09 March 2007

J. M. Tricarico ,
J. D. Johnston ,
K. A. Dawson ,
K. C. Hanson ,
K. R. McLeod  and
D. L. Harmon
J. M. Tricarico*
Affiliation:
Alltech Biotechnology Inc., Nicholasville, KY 40356, USA
J. D. Johnston
Affiliation:
Ritchie Feed & Seed Inc., Ottawa, ON K1B 4V5, Canada
K. A. Dawson
Affiliation:
Alltech Biotechnology Inc., Nicholasville, KY 40356, USA
K. C. Hanson
Affiliation:
Department of Animal Science, University of Kentucky, Lexington, KY 40546, USA
K. R. McLeod
Affiliation:
Department of Animal Science, University of Kentucky, Lexington, KY 40546, USA
D. L. Harmon
Affiliation:
Department of Animal Science, University of Kentucky, Lexington, KY 40546, USA
*
†E-mail: jtricarico@alltech.com
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Abstract

The effects of an Aspergillus oryzae extract containing alpha-amylase activity (Amaize™, Alltech Inc., Nicholasville, KY) were examined in vivo and in vitro. A lactating cow study employed 20 intact and four ruminally fistulated Holstein cows in a replicated 4 × 4 Latin-square design to examine the effects of four concentrations of dietary Amaize™ extract on milk production and composition, ruminal fermentation and serum metabolite concentrations. The treatment diets contained 0, 240, 480 or 720 alpha-amylase dextrinizing units (DU) per kg of total mixed ration (TMR) (dry-matter basis). The supplemental alpha-amylase increased the yields of milk (P = 0·02), fat (P = 0·02) and protein (P = 0·06) quadratically. The maximum milk yield was obtained when 240 DU per kg of TMR were offered. Ruminal in situ starch disappearance was not affected by alpha-amylase supplementation in lactating cows or ruminally cannulated steers. Supplemental alpha-amylase extract reduced the molar proportion of propionate in the rumen of steers (P = 0·08) and lactating cows (P = 0·04), and in rumen-simulating cultures (P = 0·04). The supplement also increased the molar proportions of acetate (P = 0·06) and butyrate (P = 0·05), and the serum beta-hydroxybutyrate (P = 0·01) and non-esterified fatty acid (P = 0·03) concentrations in lactating cows. The improvements in milk production appear to be a consequence of the effects of alpha-amylase on ruminal fermentation and the potential changes in nutrient metabolism that result from them. We conclude that supplemental alpha-amylase may be given to modify ruminal fermentation and improve milk and component yield in lactating Holstein cattle.

Keywords

Aspergillus oryzaedairy cowsmilk productionstarch
Type
Research Article
Information
Animal Science , Volume 81 , Issue 3 , December 2005 , pp. 365 - 374
Copyright
Copyright © British Society of Animal Science 2005

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