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Effects of sow nutrition on maternal and foetal serum growth factors and on foetal myogenesis

Published online by Cambridge University Press:  09 March 2007

P. M. Nissen ,
I. L. Sørensen ,
M. Vestergaard  and
N. Oksbjerg
P. M. Nissen*
Affiliation:
Department of Food Science, Danish Institute of Agricultural Sciences, PO Box 50, 8830 Tjele, Denmark
I. L. Sørensen
Affiliation:
Department of Food Science, Danish Institute of Agricultural Sciences, PO Box 50, 8830 Tjele, Denmark
M. Vestergaard
Affiliation:
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, PO Box 50, 8830 Tjele, Denmark
N. Oksbjerg
Affiliation:
Department of Food Science, Danish Institute of Agricultural Sciences, PO Box 50, 8830 Tjele, Denmark
*
†E-mail: piam.nissen@agrsci.dk
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Abstract

The objective of this study was to examine the effect of increased maternal nutrition in early to mid gestation on changes in serum growth factors of the sow and foetuses. Furthermore, the effect of the foetal sera on in vitro proliferation and differentiation of porcine primary myoblasts was examined. Pregnant sows were either given food either in accordance to requirements (2 kg/day; C) until day 50 or 70 of gestation or given food in accordance to requirements until day 25 and then ad libitum (A) until day 50 or 70. Sows were slaughtered at the Institute's veterinary controlled slaughterhouse at day 50 or 70, respectively. Serum from sows and pools of cord-blood serum from each litter were analysed for glucose, lactate, insulin, insulin-like growth factor (IGF; IGF-1 and -2) and IGF binding proteins (IGFBPs). IGF-1 (P < 0·001) was higher in A compared with C sows, and a 28-kDa IGFBP (P < 0·05) and a 24-kDa IGFBP (P < 0·05) was higher in serum from day 70 compared with day 50 sows. There was no significant effect of food intake on growth factor concentrations in foetal serum or on serum-induced proliferation and differentiation of myoblasts. A 28 kDa IGFBP, IGFBP-2 and -3 were all higher (P < 0·06) and serum-induced proliferation (P < 0·001) and differentiation (P < 0·1) lower at day 70 than day 50. Maternal food intake did not influence the DNA and RNA concentrations and the CPK activity in the foetal longissimus dorsi muscle. The glucose concentration in the liver was higher in C than A foetuses at day 70 of gestation, but not at day 50.

In conclusion, no significant effects of maternal nutrition were found on serum growth factor concentrations in the foetuses or on serum-induced proliferation and differentiation of primary myoblasts.

Keywords

insulin-like growth factor (IGF)IGF binding proteinmaternal nutritionmyogenesispigs
Type
Research Article
Information
Animal Science , Volume 80 , Issue 3 , June 2005 , pp. 299 - 306
Copyright
Copyright © British Society of Animal Science 2005

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References

Aldoretta, P. W. and Hay, W. W. 1994. Fetal nutrition. Nutrition Research 14: 929–965.CrossRefGoogle Scholar
Ashworth, C. J., Finch, A. M., Page, K. R., Nwagwu, M. O. and McArdle, H. J. 2001. Causes and consequences of fetal growth retardation in pigs. Journal of Reproduction and Fertility, Supplement 58: 233–246.Google ScholarPubMed
Bee, G. 2004. Effect of early gestation feeding, birth weight, and gender of progeny on muscle fiber characteristics of pigs at slaughter. Journal of Animal Science 82: 826–836.CrossRefGoogle ScholarPubMed
Brameld, J. M., Mostyn, A., Dandrea, J., Stephenson, T. J., Dawson, J. M., Buttery, P. J. and Symonds, M. E. 2000. Maternal nutrition alters the expression of insulin-like growth factors in fetal sheep liver and skeletal muscle. Journal of Endocrinology 167: 429–437.CrossRefGoogle ScholarPubMed
Carr, J. M., Owens, J. A., Grant, P. A., Walton, P. E., Owens, P. C. and Wallace, J. C. 1995. Circulating insulin-like growth factors (IGFs), IGF-binding proteins (IGFBPs) and tissue mRNA levels of IGFBP-2 and IGFBP-4 in the ovine fetus. Journal of Endocrinology 145: 545–557.CrossRefGoogle ScholarPubMed
Clemmons, D. R. 1991. Insulin-like growth factor binding proteins: roles in regulating IGF physiology. Journal of Developmental Physiology 15: 105–110.Google ScholarPubMed
Dwyer, C. M., Madgwick, A. J. A., Crook, A. R. and Stickland, N. C. 1992. The effect of maternal undernutrition on the growth and development of the guinea pig placenta. Journal of Developmental Physiology 18: 295–302.Google ScholarPubMed
Dwyer, C. M. and Stickland, N. C. 1991. Sources of variation in myofibre number within and between litters of pigs. Animal Production 52: 527–533.Google Scholar
Dwyer, C. M., Stickland, N. C. and Fletcher, J. M. 1994. The influence of maternal nutrition on muscle fiber number development in the porcine fetus and on subsequent postnatal growth. Journal of Animal Science 72: 911–917.CrossRefGoogle ScholarPubMed
Florini, J. R., Ewton, D. Z. and Coolican, S. A. 1996. Growth hormone and the insulin-like growth factor system in myogenesis. Endocrine Review 17: 481–517.Google ScholarPubMed
Forslid, A. and Augustinsson, O. 1988. Acidosis, hypoxia and stress hormone release in response to one-minute inhalation of 80% CO2 in swine. Acta Physiologica Scandinavica 132: 223–231.CrossRefGoogle ScholarPubMed
Frystyk, J., Dinesen, B. and Ørskov, H. 1995. Non-competitive time-resolved immunofluorometric assays for determination of human insulin-like growth factor I and II. Growth Regulation 5: 169–176.Google ScholarPubMed
Gatford, K. L., Ekert, J. E., Blackmore, K., De Blasio, M. J., Boyce, J. M., Owens, J. A., Campbell, R. G. and Owens, P. C. 2003. Variable maternal nutrition and growth hormone treatment in the second quarter of pregnancy in pigs alter semitendinosus muscle in adolescent progeny. British Journal of Nutrition 90: 283–293.CrossRefGoogle ScholarPubMed
Hossenlopp, P., Seurin, C., Segovia-Quinson, B., Harsouin, S. and Binoux, M. 1986. Analysis of serum insulin-like growth factor binding proteins using Western ligand blotting: use of the method for titration of binding proteins and competitive binding studies. Analytical Biochemistry 154: 138–143.CrossRefGoogle Scholar
Hossner, K. L., McCusker, R. H. and Dodson, M. V. 1997. Insulinlike growth factors and their binding proteins in domestic animals. Animal Science 64: 1–15.CrossRefGoogle Scholar
Jones, J. T. and Clemmons, D. R. 1995. Insulin-like growth factors and their binding proteins: biological actions. Endocrine Review 16: 3–34.Google ScholarPubMed
Kelley, R. L., Jungst, S. B., Spencer, T. E., Owsley, W. F., Rahe, C. H. and Mulvaney, D. R. 1995. Maternal treatment with somatotropin alters embryonic development and early postnatal growth of pigs. Domestic Animal Endocrinology 12: 83–94.CrossRefGoogle ScholarPubMed
Liu, J. P., Baker, J., Perkins, A. S., Robertson, E. J. and Efstratiadis, A. 1993. Mice carrying null mutations of the genes encoding insulin-like growth factor I (IGF1) and type 1 IGF receptor (IGFIr). Cell 75: 59–72.Google Scholar
Løvendahl, P. and Purup, H. M. 2002. Technical note: time-resolved fluoro-imunometric assay for intact insulin in livestock species. Journal of Animal Science 80: 191–195.CrossRefGoogle ScholarPubMed
Mashek, D. G., Ingvartsen, K. L., Andersen, J. B., Vestergaard, M. and Larsen, T. 2001. Effects of a four-day hyperinsulinemiceuglycemic clamp in early and mid-lactation dairy cows on plasma concentrations of metabolites, hormones, and binding proteins. Domestic Animal Endocrinology 21: 169–185.CrossRefGoogle ScholarPubMed
Nissen, P. M., Danielsen, V. O., Jorgensen, P. F. and Oksbjerg, N. 2003. Increased maternal nutrition of the sow has no beneficial effects of muscle fibre number or postnatal growth and has no impact on the meat quality of the offspring. Journal of Animal Science 81: 3018–3027.CrossRefGoogle ScholarPubMed
Nissen, P. M., Jorgensen, P. F. and Oksbjerg, N. 2004. Within-litter variation in muscle fiber characteristics, pig performance and meat quality traits. Journal of Animal Science 82: 414–421.CrossRefGoogle ScholarPubMed
Oksbjerg, N., Petersen, J. S., Sørensen, I. L., Henckel, P., Vestergaard, M., Ertbjerg, P., Møller, A. J., Bejerholm, C. and Støier, S. 2000. Long-term changes in performance and meat quality of Danish Landrace pigs: a study on a current compared with an unimproved genotype. Animal Science 71: 81–92.CrossRefGoogle Scholar
Osgerby, J. C., Wathes, D. C., Howard, D. and Gadd, T. S. 2002. The effect of maternal undernutrition on ovine fetal growth. Journal of Endocrinology 173: 131–141.CrossRefGoogle ScholarPubMed
Parfet, K. A. R., Lamberson, W. R., Rieke, A. R., Cantley, T. C., Ganjam, V. K., vom Saal, F. S. and Day, B. N. 1990. Intrauterine position effects in male and female swine: subsequent survivability, growth rate, morphology and semen characteristics. Journal of Animal Science 68: 179–185.CrossRefGoogle Scholar
Pedersen, P. H., Oksbjerg, N., Karlsson, A. H., Busk, H., Bendixen, E. and Henckel, P. 2001. A within litter comparison of muscle fibre characteristics and growth of halothane carrier and halothane free crossbreed pigs. Livestock Production Science 73: 15–24.CrossRefGoogle Scholar
Rehfeldt, C., Kuhn, G., Vanselow, J., Fürbass, R., Fiedler, I., Nürnberg, G., Clelland, A. K., Stickland, N. C. and Ender, K. 2001. Maternal treatment with somatotropin during early gestation affects basic events of myogenesis in pigs. Cell Tissue Research 306: 429–440.CrossRefGoogle ScholarPubMed
Rehfeldt, C., Stickland, N. C., Fiedler, L. and Wegner, J. 1999. Environmental and genetic factors as sources of variation in skeletal muscle fibre number. Basic Applied Myology 9: 235–253.Google Scholar
Sohlström, A., Katsman, A., Kind, K. L., Roberts, C. T., Owens, P. C., Robinson, J. S. and Owens, J. A. 1998. Food restriction alters pregnancy-associated changes in IGF and IGFBP in the guinea pig. American Journal of Physiology 274: E410–E416.Google ScholarPubMed
Statistical Analysis Systems Institute. 1999. User's guide. SAS Institute Inc., Cary, NC.Google Scholar
Therkildsen, M., Melchior Larsen, L. and Vestergaard, M. 2002. Influence of growth rate and muscle type on muscle fiber type characteristics, protein synthesis capacity and activity of the calpain system in Friesian calves. Animal Science 74: 243–251.CrossRefGoogle Scholar
Thissen, J. P., Ketelslegers, J. M. and Underwood, L. E. 1994. Nutritional regulation of insulin-like growth factors. Endocrine Review 15: 80–101.Google ScholarPubMed
Wigmore, P. M. C. and Stickland, N. C. 1983. Muscle development in large and small pig fetuses. Journal of Anatomy 137: 235–245.Google ScholarPubMed