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Size distribution of bovine steroidogenic luteal cells during pregnancy

Published online by Cambridge University Press:  18 August 2016

Ş Arikan  and
A. Yigit
Ş Arikan*
Affiliation:
University of Kirikkale, Faculty of Veterinary Medicine, 71100 Kirikkale, Turkey
A. Yigit
Affiliation:
University of Kirikkale, Faculty of Veterinary Medicine, 71100 Kirikkale, Turkey
*
†E-mail:sevket95@hotmail.com
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Abstract

This study was designed to investigate the size distribution of bovine steroidogenic luteal cells throughout pregnancy. Corpora lutea collected from three different stages of pregnancy were used. Luteal tissue was dissociated into single-cell suspension by enzyme treatments. Cells were stained for 3β-hydroxysteroid dehydrogenase (HSD) activity a marker for steroidogenic cells. The steroidogenic cells covered a wide spectrum of size ranging from 10 to 60 µm in diameter. There was a significant increase in mean cell diameter (P > 0·05) as pregnancy progressed. Mean diameter of 3β-HSD positive cells increased from 17·03 (s.e. 1·3) µm in the corpus luteum of early pregnancy to 33·38 (s.e. 2·4) µm in the corpus luteum of advanced pregnancy. The ratio of large (>22 µm in diameter) to small (10 to 22 µm in diameter) luteal cells was 0·32 : 1·0 in the early pregnancy, with the 10 to 22 µm cell size class predominant. However, the ratio of large to small luteal cells was increased to 6·49 : 1·0 µm as pregnancy advanced and 23 to 42 µm cell sizes become predominant. It is likely that small luteal cells develop into large cells as gestation progresses. Development of pregnancy is associated with an increase in size of steroidogenic luteal cells.

Keywords

corpus luteum, cowshydroxysteroid dehydrogenaseluteal cellspregnancy
Type
Reproduction
Information
Animal Science , Volume 73 , Issue 2 , October 2001 , pp. 323 - 327
Copyright
Copyright © British Society of Animal Science 2001

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References

Alila, H. W. and Hansel, W. 1984. Origin of different cell types in the bovine corpus luteum as characterized by specific monoclonal antibodies. Journal of Reproduction 31: 1015–1025.CrossRefGoogle ScholarPubMed
Bao, B., Thomas, M. G., Griffith, M. K., Burghardt, R. C. and Williams, G. L. 1995. Steroidogenic activity, insulin-like growth factor-I production, and proliferation of granulosa and theca cells obtained from dominant preovulatory and nonovulatory follicles during the bovine oestrous cycle: Effects of low-density and high-density lipoproteins. Biology of Reproduction 53: 1271–1279.CrossRefGoogle Scholar
Brannian, J. D., Stouffer, R. L., Shiigi, S. M. and Hoyer, P. B. 1993. Isolation of ovine luteal cell subpopulations by flow cytometry. Biology of Reproduction 48: 495–502.CrossRefGoogle ScholarPubMed
Chegini, N., Ramani, N. and Rao, C. V. 1984. Morphological and biochemical characterisation of small and large bovine luteal cells during pregnancy. Molecular and Cellular Endocrinology 37: 89–102.CrossRefGoogle ScholarPubMed
Estergreen, V. L., Frost, O. L., Gomes, W. R., Erb, R. E. and Bullard, J. F. 1967. Effect of ovariectomy on pregnancy maintenance and parturition in dairy cows. Journal of Dairy Science 50: 1293–1295.Google Scholar
Fields, M. J. and Fields, P. A. 1996. Morphological characteristics of the bovine corpus luteum during the oestrous cycle and pregnancy. Theriogenology 45: 1295–1325.CrossRefGoogle Scholar
Fitz, T. A., Mayan, M. H., Sawyer, H. R. and Niswender, G. D. 1982. Characterisation of two steroidogenic cell types in the ovine corpus luteum. Biology of Reproduction 27: 703–711.CrossRefGoogle ScholarPubMed
Hansel, W., Alila, H. W., Dowd, J. P. and Yang, X. 1987. Control of steroidogenesis in small and large bovine luteal cells. Australian Journal of Biological Science 40: 331–347.CrossRefGoogle ScholarPubMed
Hild-Petrito, S.A, Shiigi, S. M. and Stouffer, R. L. 1989. Isolation and characterisation of cell subpopulations from the monkey corpus luteum of the menstrual cycle. Biology of Reproduction 40: 1075–1085.CrossRefGoogle Scholar
Hopkins, S. M. 1989. Veterinary endocrinology and reproduction (ed. McDonald, L. E.), pp. 402. Lea and Febiger, London.Google Scholar
Hoyer, P. B., Keyes, P. L. and Nisvender, G. D. 1986. Size distribution and hormonal responsiveness of dispersed rabbit luteal cells during pseudopregnancy. Biology of Reproduction 34: 905–910.CrossRefGoogle ScholarPubMed
Jainudeen, M. R. and Hafez, E. S. E. 1993. Gestation, prenatal physiology, and parturition. In Reproduction in farm animals (ed. Hafez, E. S. E.), pp. 213–237. Lea and Febiger, Pennsylvania.Google Scholar
Joseph, M. M. and Mead, R. A. 1988. Size distribution of ferret luteal cells during pregnancy. Biology of Reproduction 39: 1159–1169.CrossRefGoogle ScholarPubMed
Kuarnaga, E., Kanuka, H., Hirabayashi, K., Suzuki, M., Nishihara, M. and Takahashi, M. 2000. Progesterone is a cell death suppressor that downregulates Fas expression in rat corpus luteum. FEBS Letters 466: 279–282.CrossRefGoogle Scholar
Lei, Z. M., Chegini, N. and Rao, C. H. V. 1991. Quantitative cell composition of human and bovine corpora lutea from various reproductive states. Biology of Reproduction 44: 1148–1156.CrossRefGoogle ScholarPubMed
Miyauchi, F. and Midgley, A. R. 1990. Morphologically and functionally distinct subpopulations of steroidogenic cells in corpora lutea during pregnancy in rats. Endocrinologia Japonica 37: 649–663.CrossRefGoogle ScholarPubMed
Musah, A. I., Schwabe, C. and Anderson, L. L. 1990. Relaxin, oxytocin, and prostaglandin effects on progesterone secretion from bovine luteal cells during different stages of gestation. Proceedings of the Society for Experimental Biology and Medicine 195: 255–260.CrossRefGoogle ScholarPubMed
O’Shaughnessy, P. J. and Wathes, D. C. 1985. Characteristics of bovine luteal cells in culture: morphology, proliferation and progesterone secretion in different media and effects of LH, dibutyryl cyclic AMP, antioxidants and insulin. Journal of Endocrinology 104: 355–361.CrossRefGoogle ScholarPubMed
O’Shea, J.D., Rodgers, R. J. and D’Occhio, M. J. 1989. Cellular composition of the cyclic corpus luteum of the cow. Journal of Reproduction and Fertility 85: 483–487.CrossRefGoogle ScholarPubMed
Richardson, C., Jones, P. C., Barnard, V., Heber, C. N., Terlecki, S. and Wijeratne, W. V. S. 1990. Estimation of the developmental age of the bovine foetus and newborn calf. Veterinary Record 24: 279–284.Google Scholar
Rodgers, R. J., Mitchell, M. D. and Simpson, E. R. 1988. Secretion of progesterone and prostaglandins by cells of bovine corpora lutea from three stages of the luteal phase. Journal of Endocrinology 118: 121–126.CrossRefGoogle ScholarPubMed
Schwall, R. H., Gamboni, F., Mayan, M. H. and Niswender, G. D. 1986. Changes in the distribution of sizes of ovine luteal cells during the oestrus cycle. Biology of Reproduction 34: 911–916.CrossRefGoogle Scholar
Thomsen, J. L. 1975. Body length, head circumference, and weight of bovine foetuses: prediction of gestational age. Journal of Dairy Science 58: 1371–1373.Google Scholar
Weber, D. M., Fields, P. A., Romrell, L. J., Tumwasorn, S., Ball, B. A., Drost, M. and Fields, M. J. 1987. Functional differences between small and large luteal cells of the late-pregnant vs. non-pregnant cow. Biology of Reproduction 37: 685–697.CrossRefGoogle Scholar
Webly, G. E., Richardson, M. C., Smith, C. A., Masson, G. M. and Hearn, J. P. 1990. Size distribution of luteal cells from pregnant and non-pregnant marmoset monkeys and a comparison of the morphology of marmoset luteal cells with those from the human corpus luteum. Journal of Reproduction and Fertility 90: 437–457.Google Scholar
Wilkinson, R. F., Anderson, E. and Aalberg, J. 1976. Cytological observations of dissociated rat corpus luteum. Journal of Ultrastructure Research 57: 168–184.Google Scholar