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α 1-acid glycoprotein inhibits lipogenesis in neonatal swine adipose tissue1

Published online by Cambridge University Press:  26 November 2015

T. G. Ramsay ,
L. Blomberg  and
T. J. Caperna
T. G. Ramsay*
Affiliation:
Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
L. Blomberg
Affiliation:
Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
T. J. Caperna
Affiliation:
Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
*
†E-mail: timothy.ramsay@ars.usda.gov
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Abstract

Serum α1-acid glycoprotein (AGP) is elevated during late gestation and at birth in the pig and rapidly declines postnatally. In contrast, the pig is born with minimal lipid stores in the adipose tissue, but rapidly accumulates lipid during the first week. The present study examined if AGP can affect adipose tissue metabolism in the neonatal pig. Isolated cell cultures or tissue explants were prepared from dorsal subcutaneous adipose tissue of preweaning piglets. Porcine AGP was used at concentrations of 0, 100, 1000 and 5000 ng/ml medium in 24 h incubations. AGP reduced the messenger RNA (mRNA) abundance of the lipogenic enzymes, malic enzyme (ME), fatty acid synthase and acetyl coA carboxylase by at least 40% (P<0.001). The activity of ME and citrate lyase were also reduced by AGP (P<0.05). Glucose oxidation was reduced by treatment with 5000 ng AGP/ml medium (P<0.05). The 14C-glucose incorporation into fatty acids was reduced by ~25% by AGP treatment for 24 h with 1000 ng AGP/ml medium (P<0.05). The decrease in glucose metabolism by AGP appears to function through an inhibition in insulin-mediated glucose oxidation and incorporation into fatty acids. This was supported by the analysis of the mRNA abundance for sterol regulatory element-binding protein (SREBP), carbohydrate regulatory element-binding protein (ChREBP) and insulin receptor substrate 1 (IRS1), which all demonstrated reductions of at least 23% in response to AGP treatment (P<0.05). These data demonstrate an overall suppression of lipogenesis due to AGP inhibition of lipogenic gene expression in vitro, which the metabolic data and SREBP, ChREBP and IRS1 gene expression analysis suggest is through an inhibition in insulin-mediated events. Second, these data suggest that AGP may contribute to limiting lipogenesis within adipose tissue during the perinatal period, as AGP levels are highest for any serum protein at birth.

Keywords

lipogenesisα 1-acid glycoproteinneonateswineadipose
Type
Research Article
Information
animal , Volume 10 , Issue 5 , May 2016 , pp. 812 - 820
Copyright
© The Animal Consortium 2015. This is a work of the U.S. Government and is not subject to copyright protection in the United States. 

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Footnotes

1

Mention of trade name, proprietary product or vendor does not constitute a guarantee or warranty of the product by the US Department of Agriculture or imply its approval to the exclusion of other products or vendors that also may be suitable.

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