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Aneuploidy in dizygotic twin sheep detected using genome-wide single nucleotide polymorphism data from two commonly used commercial vendors

Published online by Cambridge University Press:  15 March 2018

D. P. Berry ,
A. O’Brien ,
J. O’Donovan ,
N. McHugh ,
E. Wall ,
S. Randles ,
K. McDermott ,
R. E. O’Connor ,
M. A. Patil  and
J. Ho
...Show all authors
D. P. Berry*
Affiliation:
Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork P61 C996, Ireland
A. O’Brien
Affiliation:
Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork P61 C996, Ireland
J. O’Donovan
Affiliation:
Department of Agriculture, Food and the Marine, Regional Veterinary Laboratory, Model Farm Road, CorkT12 XD51, Ireland
N. McHugh
Affiliation:
Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork P61 C996, Ireland
E. Wall
Affiliation:
Sheep Ireland, Bandon, Co. Cork P72 X050, Ireland
S. Randles
Affiliation:
Sheep Ireland, Bandon, Co. Cork P72 X050, Ireland
K. McDermott
Affiliation:
Sheep Ireland, Bandon, Co. Cork P72 X050, Ireland
R. E. O’Connor
Affiliation:
University of Kent, School of Biosciences, University of Kent, Canterbury, CT2 7AF, UK
M. A. Patil
Affiliation:
Thermo Fisher Scientific, 3450 Central Expressway, Santa Clara, CA 95051, USA
J. Ho
Affiliation:
Thermo Fisher Scientific, 3450 Central Expressway, Santa Clara, CA 95051, USA
A. Kennedy
Affiliation:
Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork P61 C996, Ireland
N. Byrne
Affiliation:
Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork P61 C996, Ireland
D. C. Purfield
Affiliation:
Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork P61 C996, Ireland
*
†E-mail: Donagh.berry@teagasc.ie
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Abstract

Early detection of karyotype abnormalities, including aneuploidy, could aid producers in identifying animals which, for example, would not be suitable candidate parents. Genome-wide genetic marker data in the form of single nucleotide polymorphisms (SNPs) are now being routinely generated on animals. The objective of the present study was to describe the statistics that could be generated from the allele intensity values from such SNP data to diagnose karyotype abnormalities; of particular interest was whether detection of aneuploidy was possible with both commonly used genotyping platforms in agricultural species, namely the Applied BiosystemsTM AxiomTM and the Illumina platform. The hypothesis was tested using a case study of a set of dizygotic X-chromosome monosomy 53,X sheep twins. Genome-wide SNP data were available from the Illumina platform (11 082 autosomal and 191 X-chromosome SNPs) on 1848 male and 8954 female sheep and available from the AxiomTM platform (11 128 autosomal and 68 X-chromosome SNPs) on 383 female sheep. Genotype allele intensity values, either as their original raw values or transformed to logarithm intensity ratio (LRR), were used to accurately diagnose two dizygotic (i.e. fraternal) twin 53,X sheep, both of which received their single X chromosome from their sire. This is the first reported case of 53,X dizygotic twins in any species. Relative to the X-chromosome SNP genotype mean allele intensity values of normal females, the mean allele intensity value of SNP genotypes on the X chromosome of the two females monosomic for the X chromosome was 7.45 to 12.4 standard deviations less, and were easily detectable using either the AxiomTM or Illumina genotype platform; the next lowest mean allele intensity value of a female was 4.71 or 3.3 standard deviations less than the population mean depending on the platform used. Both 53,X females could also be detected based on the genotype LRR although this was more easily detectable when comparing the mean LRR of the X chromosome of each female to the mean LRR of their respective autosomes. On autopsy, the ovaries of the two sheep were small for their age and evidence of prior ovulation was not appreciated. In both sheep, the density of primordial follicles in the ovarian cortex was lower than normally found in ovine ovaries and primary follicle development was not observed. Mammary gland development was very limited. Results substantiate previous studies in other species that aneuploidy can be readily detected using SNP genotype allele intensity values generally already available, and the approach proposed in the present study was agnostic to genotype platform.

Keywords

mutationchromosomeovineturnerkaryotype
Type
Research Article
Information
animal , Volume 12 , Issue 12 , December 2018 , pp. 2462 - 2469
Copyright
© The Animal Consortium 2018 

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