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Bioavailability of α-tocopherol stereoisomers in lambs depends on dietary doses of all-rac- or RRR-α-tocopheryl acetate

Published online by Cambridge University Press:  27 December 2018

L. N. Leal Open the ORCID record for L. N. Leal [Opens in a new window] ,
S. K. Jensen ,
J. M. Bello ,
L. A. Den Hartog ,
W. H. Hendriks  and
J. Martín-Tereso
L. N. Leal*
Affiliation:
Trouw Nutrition Research and Development, P.O. Box 299, Amersfoort3800-AG, The Netherlands Animal Nutrition Group, Department of Animal Sciences, Wageningen University & Research, De Elst 1, Wageningen 6708 WD, The Netherlands
S. K. Jensen
Affiliation:
Department of Animal Science, Aarhus University, Box 50, TjeleDK-8830, Denmark
J. M. Bello
Affiliation:
Nanta S.A., Ronda de Poniente 9, 28460 Tres Cantos, Madrid, Spain
L. A. Den Hartog
Affiliation:
Trouw Nutrition Research and Development, P.O. Box 299, Amersfoort3800-AG, The Netherlands Animal Nutrition Group, Department of Animal Sciences, Wageningen University & Research, De Elst 1, Wageningen 6708 WD, The Netherlands
W. H. Hendriks
Affiliation:
Animal Nutrition Group, Department of Animal Sciences, Wageningen University & Research, De Elst 1, Wageningen 6708 WD, The Netherlands
J. Martín-Tereso
Affiliation:
Trouw Nutrition Research and Development, P.O. Box 299, Amersfoort3800-AG, The Netherlands
*
†E-mail: leonel.leal@trouwnutrition.com
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Abstract

When supplementing lamb diets with vitamin E, an equivalence factor of 1.36 is used to discriminate between RRR-α-tocopheryl acetate and all-rac-α-tocopheryl acetate. However, more recent studies suggest a need for new equivalence factors for livestock animals. The current study aimed to determine the effect of RRR- and all-rac-α-tocopheryl acetate supplementation on α-tocopherol deposition in lamb tissues. A total of 108 Rasa Aragonesa breed lambs were fed increasing amounts of all-rac-α-tocopheryl acetate (0.25, 0.5, 1.0 and 2.0 g/kg compound feed) or RRR-α-tocopheryl acetate (0.125, 0.25, 0.5 and 1.0 g/kg compound feed) by adding them to a basal diet that contained 0.025 g/kg feed of all-rac-α-tocopheryl acetate as part of the standard vitamin and mineral mixture. The diets were fed for the last 14 days before slaughtering at 25.8±1.67 kg BW. Within 20 min after slaughter samples of muscle, heart, liver, brain and spleen were frozen at −20°C until α-tocopherol analysis. Increased supplementation of either vitamin E sources led to a significant increase (P < 0.001) in α-tocopherol concentration in all tissues studied. The tissue with the highest α-tocopherol concentration was the liver followed by spleen, heart and muscle. At similar supplementation levels (0.25, 0.50 and 1.0 g/kg compound feed), α-tocopherol content in the selected tissues was not affected by α-tocopherol source. However, the ratios between RRR- and all-rac-α-tocopheryl acetate increased with the increasing α-tocopherol supplementation (at 0.25 and 1.0 g/kg compound feed), from 1.06 to 1.16 in muscle, 1.07 to 1.15 in heart, 0.91 to 0.94 in liver and 0.98 to 1.10 in spleen. The highest relative proportion of Ʃ2S (sum of SSS-, SSR-, SRS- and SRR-α-tocopherol)-configured stereoisomers was found in the liver of lambs supplemented with all-rac-α-tocopheryl acetate accounting for up to 35 to 39% of the total α-tocopherol retained, whereas the proportion of Ʃ2S-configured stereoisomers in the other tissues accounted for <14%. Increasing all-rac-α-tocopheryl acetate supplementation was also found to affect the 2R-configured stereoisomer profile in muscle, heart and spleen with increasing proportions of RRS-, RSR- and RSS- at the cost of RRR-α-tocopherol. In all tissues, the relative proportion of all non-RRR-stereoisomers in lambs receiving RRR-α-tocopheryl acetate was lower than RRR-α-tocopherol. These results confirm that the relative bioavailability of RRR- and all-rac-α-tocopheryl acetate is dose- and tissue-dependent and that a single ratio to discriminate the two sources cannot be used.

Keywords

natural vitamin Esynthetic vitamin Eantioxidantbioequivalencetissue distribution
Type
Research Article
Information
animal , Volume 13 , Issue 9 , September 2019 , pp. 1874 - 1882
Copyright
© The Animal Consortium 2018 

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References

Arnold, RN, Scheller, KK, Arp, SC, Williams, SN, Buege, DR and Schaefer, DM 1992. Effect of long- or short-term feeding of alpha-tocopheryl acetate to Holstein and crossbred beef steers on performance, carcass characteristics, and beef color stability. Journal of Animal Science 70, 3055–3065.CrossRefGoogle ScholarPubMed
Blatt, DH, Pryor, WA, Mata, JE and Rodriguez-Proteau, R 2004. Re-evaluation of the relative potency of synthetic and natural alpha-tocopherol: experimental and clinical observations. Journal of Nutritional Biochemistry 15, 380–395.CrossRefGoogle ScholarPubMed
Bramley, PM, Elmadfa, I, Kafatos, A, Kelly, FJ, Manios, Y, Roxborough, HE, Schuch, W, Sheehy, PJA and Wagner, KH 2000. Vitamin E. Journal of the Science of Food and Agriculture 80, 913–938.3.0.CO;2-3>CrossRefGoogle Scholar
Brigelius-Flohe, R, Kelly, FJ, Salonen, JT, Neuzil, J, Zingg, JM and Azzi, A 2002. The European perspective on vitamin E: current knowledge and future research. American Journal of Clinical Nutrition 76, 703–716.CrossRefGoogle ScholarPubMed
Brigelius-Flohe, R and Traber, MG 1999. Vitamin E: function and metabolism. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology 13, 1145–1155.CrossRefGoogle ScholarPubMed
Burton, GW, Trabet, MG, Acuff, RV, Walters, DN, Kayden, H, Hughes, L and Ingold, KU 1998. Human plasma and tissue α-tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E. American Journal of Clinical Nutrition 67, 669–684.CrossRefGoogle ScholarPubMed
Clifford, AJ, De Moura, FF, Ho, CC, Chuang, JC, Follett, J, Fadel, JG and Novotny, JA 2006. A feasibility study quantifying in vivo human α-tocopherol metabolism. American Journal of Clinical Nutrition 84, 1430–1441.CrossRefGoogle ScholarPubMed
De la Fuente, J, Díaz, MT, Álvarez, I, Lauzurica, S, Cañeque, V and Pérez, C 2007. Effect of dietary supplementation with vitamin E on characteristics of vacuum-packed lamb. Journal of the Science of Food and Agriculture 87, 651–659.CrossRefGoogle Scholar
Drotleff, AM and Ternes, W 2001. Determination of RS,E/Z-tocotrienols by HPLC. Journal of Chromatography A 909, 215–223.CrossRefGoogle ScholarPubMed
Harris, PL and Ludwig, MI 1949. Relative vitamin E potency of natural and of synthetic alpha-tocopherol. Journal of Biological Chemistry 179, 1111–1115.Google ScholarPubMed
Hidiroglou, M 1987. Vitamin E levels in sheep tissues at various times after a single oral administration of d-alpha-tocopherol acetate. International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernáhrungsforschung. Journal International de Vitaminologie et de Nutrition 57, 381–384.Google Scholar
Ingold, KU, Burton, GW, Foster, DO, Hughes, L, Lindsay, DA and Webb, A 1987. Biokinetics of and discrimination between dietary RRR- and SRR-α-tocopherols in the male rat. Lipids 22, 163–172.CrossRefGoogle ScholarPubMed
Jensen, SK, Engberg, RM and Hedemann, MS 1999. All-rac-alpha-tocopherol acetate is a better vitamin E source than all-rac-alpha-tocopherol succinate for broilers. Journal of Nutrition 129, 1355–1360.CrossRefGoogle ScholarPubMed
Jensen, SK and Lauridsen, C 2007. Alpha-tocopherol stereoisomers. Vitamins and Hormones 76, 281–308.CrossRefGoogle ScholarPubMed
Jensen, SK, Norgaard, JV and Lauridsen, C 2006. Bioavailability of alpha-tocopherol stereoisomers in rats depends on dietary doses of all-rac- or RRR-alpha-tocopheryl acetate. British Journal of Nutrition 95, 477–487.CrossRefGoogle ScholarPubMed
Lanari, MC, Brewster, M, Yang, A and Tume, RK 2002. Pasture and grain finishing affect the color stability of beef. Journal of Food Science 67, 2467–2473.CrossRefGoogle Scholar
Lauridsen, C, Engel, H, Craig, AM and Traber, MG 2002. Relative bioactivity of dietary RRR- and all-rac-alpha-tocopheryl acetates in swine assessed with deuterium-labeled vitamin E. Journal of Animal Science 80, 702–707.CrossRefGoogle ScholarPubMed
Leal, LN, Beltrán, JA, Alonso, V, Bello, JM, den Hartog, LA, Hendriks, WH and Martín-Tereso, J 2018. Dietary vitamin E dosage and source affects meat quality parameters in light weight lambs. Journal of the Science of Food and Agriculture 98, 1606–1614.CrossRefGoogle ScholarPubMed
Leal, LN, Jensen, SK, Bello, JM, Den Hartog, LA, Hendriks, WH and Martín-Tereso, J 2017. Supplementation of natural or synthetic vitamin E on α-tocopherol concentration in lamb tissues. Paper presented at the 68th Annual meeting of the European Federation of Animal Science, 28 August to 1 September 2017, Tallinn, Estonia, pp. 355.Google Scholar
Lee, SK, Panjono Kang, SM, Kim, TS and Park, YS 2008. The effects of dietary sulfur and Vitamin E supplementation on the quality of beef from the longissimus muscle of Hanwoo bulls. Asian-Australasian Journal of Animal Sciences 21, 1059–1066.CrossRefGoogle Scholar
López-Bote, CJ, Daza, A, Soares, M and Berges, E 2001. Dose-response effect of dietary vitamin E concentration on meat quality characteristics in light-weight lambs. Animal Science 73, 451–457.CrossRefGoogle Scholar
Nassu, RT, Dugan, ME, Juarez, M, Basarab, JA, Baron, VS and Aalhus, JL 2011. Effect of alpha-tocopherol tissue levels on beef quality. Animal 5, 2010–2018.CrossRefGoogle ScholarPubMed
Ochoa, L, McDowell, LR, Williams, SN, Wilkinson, N, Boucher, J and Lentz, EL 1992. Alpha-Tocopherol concentrations in serum and tissues of sheep fed different sources of vitamin E. Journal of Animal Science 70, 2568–2573.CrossRefGoogle ScholarPubMed
Realini, CE, Duckett, SK, Brito, GW, Dalla Rizza, M and De Mattos, D 2004. Effect of pasture vs. concentrate feeding with or without antioxidants on carcass characteristics, fatty acid composition, and quality of Uruguayan beef. Meat Science 66, 567–577.CrossRefGoogle ScholarPubMed
Ripoll, G, González-Calvo, L, Molino, F, Calvo, JH and Joy, M 2013. Effects of finishing period length with vitamin E supplementation and alfalfa grazing on carcass color and the evolution of meat color and the lipid oxidation of light lambs. Meat Science 93, 906–913.CrossRefGoogle ScholarPubMed
Traber, MG 1996. Regulation of human plasma vitamin E. Advances in Pharmacology 38, 49–63.CrossRefGoogle Scholar
Traber, MG, Mah, E, Leonard, SW, Bobe, G and Bruno, RS 2017. Metabolic syndrome increases dietary alpha-tocopherol requirements as assessed using urinary and plasma vitamin E catabolites: a double-blind, crossover clinical trial. American Journal of Clinical Nutrition 105, 571–579.CrossRefGoogle ScholarPubMed
Traber, MG, Sokol, RJ, Burton, GW, Ingold, KU, Papas, AM, Huffaker, JE and Kayden, HJ 1990. Impaired ability of patients with familial isolated vitamin E deficiency to incorporate α-tocopherol into lipoproteins secreted by the liver. Journal of Clinical Investigation 85, 397–407.CrossRefGoogle ScholarPubMed
Turner, KE, McClure, KE, Weiss, WP, Borton, RJ and Foster, JG 2002. Alpha-tocopherol concentrations and case life of lamb muscle as influenced by concentrate or pasture finishing. Journal of Animal Science 80, 2513–2521.Google ScholarPubMed
USP 1979. The United States Pharmacopeia. National Formulary. United States Pharmacopeial Convention, Rockville, MD, USA.Google Scholar
Weiser, H and Vecchi, M 1981. Stereoisomers of alpha-tocopheryl acetate. Characterization of the samples by physico-chemical methods and determination of biological activities in the rat resorption-gestation test. International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernáhrungsforschung. Journal International de Vitaminologie et de. Nutrition 51, 100–113.Google Scholar
Weiser, H and Vecchi, M 1982. Stereoisomers of alpha-tocopheryl acetate. II. Biopotencies of all eight stereoisomers, individually or in mixtures, as determined by rat resorption-gestation tests. International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernáhrungsforschung. Journal International de Vitaminologie et de Nutrition 52, 351–370.Google ScholarPubMed
Wolf, G 2006. How an increased intake of alpha-tocopherol can suppress the bioavailability of gamma-tocopherol. Nutrition Reviews 64, 295–299.CrossRefGoogle ScholarPubMed
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