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Circulating extracellular miR-22, miR-155, and miR-365 as candidate biomarkers to assess transport-related stress in turkeys

Published online by Cambridge University Press:  13 January 2016

C. Lecchi ,
A. T. Marques ,
M. Redegalli ,
S. Meani ,
L. J. Vinco ,
V. Bronzo  and
F. Ceciliani
C. Lecchi*
Affiliation:
Department of Veterinary Science and Public Health, Università degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
A. T. Marques
Affiliation:
Department of Veterinary Science and Public Health, Università degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
M. Redegalli
Affiliation:
Department of Veterinary Science and Public Health, Università degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
S. Meani
Affiliation:
Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy
L. J. Vinco
Affiliation:
Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy
V. Bronzo
Affiliation:
Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
F. Ceciliani
Affiliation:
Department of Veterinary Science and Public Health, Università degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
*
†E-mail: cristina.lecchi@unimi.it
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Abstract

MicroRNA (miRNA) have been identified in circulating blood and might have the potential to be used as biomarkers for several pathophysiological conditions. To identify miRNA that are altered following stress events, turkeys (Meleagris gallopavo) were subjected to 2 h of road transportation. The expression levels of five circulating miRNA, namely miR-22, miR-155-5p, miR-181a-3p, miR-204 and miR-365-3p, were detected and assessed by quantitative polymerase chain reaction using TaqMan® probes, as potential biomarkers of stress. The areas under the receiver operating characteristic curves were then used to evaluate the diagnostic performance of miRNA. A panel of three stress-responsive miRNA, miR-22, miR-155 and miR-365 were identified; their expression levels were significantly higher after road transportation and the area under the curve (AUC) were 0.763, 0.71 and 0.704, respectively. Combining the three miRNA a specificity similar to the one found for the three miRNA separately was found. The AUC of the weighted average of the three miRNA was 0.763. This preliminary study suggests that the expression levels of circulating miR-22, miR-155 and miR-365 are increased during transport-related stress and that they may have diagnostic value to discriminate between stressed- and unstressed animals.

Keywords

circulating miRNAturkeystresswelfarebiomarkers
Type
Short Communication
Information
animal , Volume 10 , Issue 7 , July 2016 , pp. 1213 - 1217
Copyright
© The Animal Consortium 2016 

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