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Dietary starch and rhubarb supplement increase ruminal dissolved hydrogen without altering rumen fermentation and methane emissions in goats

Published online by Cambridge University Press:  08 October 2018

M. Wang ,
R. Wang ,
M. Liu ,
K. A. Beauchemin ,
X. Z. Sun ,
S. X. Tang ,
J. Z. Jiao ,
Z. L. Tan  and
Z. X. He
M. Wang
Affiliation:
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan410128, P. R. China
R. Wang
Affiliation:
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan410128, P. R. China College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan410128, P. R. China
M. Liu
Affiliation:
College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan410128, P. R. China
K. A. Beauchemin
Affiliation:
Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AlbertaT1J 4B1,Canada
X. Z. Sun
Affiliation:
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, Jilin132101, P. R. China
S. X. Tang
Affiliation:
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan410128, P. R. China
J. Z. Jiao
Affiliation:
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan410128, P. R. China
Z. L. Tan*
Affiliation:
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan410128, P. R. China
Z. X. He
Affiliation:
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan410125, P. R. China Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha, Hunan410128, P. R. China
*
†E-mail: zltan@isa.ac.cn
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Abstract

Hydrogen is an important intermediate that is produced during carbohydrate fermentation to volatile fatty acid and utilized by methanogens to produce methane in the rumen. Ruminal volatile fatty acid and dissolved methane concentrations are more than 500 times greater than dissolved hydrogen concentration. Therefore, we hypothesized that dissolved hydrogen might have a higher sensitivity in response to dietary changes compared with volatile fatty acid and dissolved methane. Using goats, we investigated the effects of increasing dietary starch content (maize replaced with wheat bran) and supplementing with rhubarb rhizomes and roots on the relationships among dissolved hydrogen, dissolved methane and other fermentation end products. The study was conducted in a replicated 4×4 Latin square with a 2×2 factorial arrangement of four treatments: two starch levels (220 v. 320 g/kg dry matter (DM)), without and with rhubarb supplement (0% v. 2.8% of total mixed ration). Increased dietary starch and rhubarb supplementation did not alter volatile fatty acid concentrations or methane emissions in terms of g/day, g/g DM intake and g/g organic matter digested. However, goats fed the high-starch diet had greater dissolved hydrogen (P=0.005) and relative abundance of Selenomonas ruminantium (P<0.01), and lower (P=0.02) copy number of protozoa than those fed the low-starch diet. Rhubarb increased ruminal dissolved H2 (P=0.03) and total volatile fatty acid concentration (P<0.001), but decreased copies of bacteria (P=0.002). In conclusion, dissolved hydrogen appears to be more sensitive to dietary changes with starch content and rhubarb supplementation, when compared with volatile fatty acid concentrations and methane production.

Keywords

fermentation pathwaysrumen microbiotadissolved gasescarbohydratemethane inhibitor
Type
Research Article
Information
animal , Volume 13 , Issue 5 , May 2019 , pp. 975 - 982
Copyright
© Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada 2018. 

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