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Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Published online by Cambridge University Press:  01 January 2008

A. K. Bansal  and
G. S. Bilaspuri
A. K. Bansal*
Affiliation:
Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India
G. S. Bilaspuri
Affiliation:
Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India
*
E-mail: bansal2amrit@yahoo.co.in
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Abstract

Numerous factors influence male fertility, one of these being the oxidative stress, which has elicited enormous interest recently. In sperm, induction of oxidation decreases motility and viability but increases lipid peroxidation (LPO). The optimum dose of ferrous ascorbate (FeAA: FeSO4 + ascorbic acid) for inducing oxidative stress by affecting motility, viability and LPO has been ascertained in local crossbred cattle bull spermatozoa. The fractions of spermatozoa suspended in 2.9% sodium citrate were subjected to three doses of FeAA (100 : 500, 150 : 750, 200 : 1000; μmol/l FeSO4 : μmol/l ascorbic acid). These fractions were assessed for various parameters. Increase in the incubation period and promoter concentration induced a decrease in motility and viability, but an increase in LPO. Among three doses of FeAA, 150 : 750 μmol/l ascorbic acid is suggested to be the optimum/best dose as it induces the oxidative stress/LPO to a significant extent and also maintains better motility and viability as compared with the other two doses, and such conditions may enhance the fertilising potential of bull spermatozoa.

Keywords

ferrous ascorbatelipid peroxidationreactive oxygen speciessperm motilityviability
Type
Full Paper
Information
animal , Volume 2 , Issue 1 , January 2008 , pp. 100 - 104
Copyright
Copyright © The Animal Consortium 2008

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