Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-27T23:35:31.054Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of selected plant species within biodiverse pasture on in vitro fatty acid biohydrogenation and tissue fatty acid composition of lamb

Published online by Cambridge University Press:  22 February 2018

K. E. Kliem ,
A. L. Thomson ,
L. A. Crompton  and
D. I. Givens
K. E. Kliem*
Affiliation:
Animal Dairy and Food Chain Sciences, School of Agriculture, Policy and Development, University of Reading, Reading, RG6 6AR, UK
A. L. Thomson
Affiliation:
Animal Dairy and Food Chain Sciences, School of Agriculture, Policy and Development, University of Reading, Reading, RG6 6AR, UK
L. A. Crompton
Affiliation:
Animal Dairy and Food Chain Sciences, School of Agriculture, Policy and Development, University of Reading, Reading, RG6 6AR, UK
D. I. Givens
Affiliation:
Institute for Food Nutrition and Health, University of Reading, Reading, RG6 6AR, UK
*
†E-mail: k.e.kliem@reading.ac.uk
Get access

Abstract

The effect of botanical diversity on supply of polyunsaturated fatty acids (PUFA) to ruminants in vitro, and the fatty acid (FA) composition of muscle in lambs was investigated. Six plant species, commonly grown as part of UK herbal ley mixtures (Trifolium pratense, Lotus corniculatus, Achillea millefolium, Centaurea nigra, Plantago lanceolata and Prunella vulgaris), were assessed for FA profile, and in vitro biohydrogenation of constituent PUFA, to estimate intestinal supply of PUFA available for absorption by ruminants. Modelling the in vitro data suggested that L. corniculatus and P. vulgaris had the greatest potential to increase 18:3n-3 supply to ruminants, having the highest amounts escaping in vitro biohydrogenation. Biodiverse pastures were established using the six selected species, under-sown in a perennial ryegrass-based sward. Lambs were grazed (~50 days) on biodiverse or control pastures and the effects on the FA composition of musculus longissimus thoracis (lean and subcutaneous fat) and musculus semimembranosus (lean) were determined. Biodiverse pasture increased 18:2n-6 and 18:3n-3 contents of m. semimembranosus (+14.8 and +7.2 mg/100 g tissue, respectively) and the subcutaneous fat of m. longissimus thoracis (+158 and +166 mg/100 g tissue, respectively) relative to feeding a perennial ryegrass pasture. However, there was no effect on total concentrations of saturated FA in the tissues studied. It was concluded that enhancing biodiversity had a positive impact on muscle FA profile reflected by increased levels of total PUFA.

Keywords

biodiversitymultispecies swardsfatty acidsbiohydrogenationlamb muscle
Type
Research Article
Information
animal , Volume 12 , Issue 11 , November 2018 , pp. 2415 - 2423
Copyright
© The Animal Consortium 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alcaide, EM, García, AIM and Aguilera, JF 2000. A comparative study of nutrient digestibility, kinetics of degradation and passage and rumen fermentation pattern in goats and sheep offered good quality diets. Livestock Production Science 64, 215–223.Google Scholar
Azuhnwi, BN, Boller, B, Dohme-Meier, F, Hess, HD, Kreuzer, M, Stringano, E and Mueller-Harvey, I 2013. Exploring variation in proanthocyanidin composition and content of sainfoin (Onobrychis viciifolia). Journal of the Science of Food and Agriculture 93, 2102–2109.Google Scholar
Barry, TN and Manley, TR 1986. Interrelationships between the concentrations of total condensed tannin, free condensed tannin and lignin in lotus sp. and other possible consequences in ruminant nutrition. Journal of the Science of Food and Agriculture 37, 248–254.Google Scholar
Boufaïed, H, Chouinard, PY, Tremblay, GF, Petit, HV, Michaud, R and Bélanger, G 2003. Fatty acids in forages. II. In vitro ruminal biohydrogenation of linolenic and linoleic acids from timothy. Canadian Journal of Animal Science 83, 513–522.Google Scholar
Burdge, GC and Calder, PC 2005. Alpha-linolenic acid metabolism in humans: the effects of gender and age on conversion to longer-chain polyunsaturated fatty acids. European Journal of Lipid Science and Technology 107, 426–439.Google Scholar
Campidonico, L, Toral, PG, Priolo, A, Luciano, G, Valenti, B, Hervas, G, Frutos, P, Copani, G, Ginane, C and Niderkorn, V 2016. Fatty acid composition of ruminal digesta and longissimus muscle from lambs fed silage mixtures including red clover, sainfoin, and timothy. Journal of Animal Science 94, 1550–1560.Google Scholar
Collomb, M, Bütikofer, U, Sieber, R, Jeangros, B and Bosset, JO 2002. Correlation between fatty acids in cows’ milk fat produced in the lowlands, mountains and highlands of Switzerland and botanical composition of the fodder. International Dairy Journal 12, 661–666.Google Scholar
Department for Environment, Food and Rural Affairs (DEFRA) 2004. Sward enhancement: diversifying grassland by oversowing and slot seeding. Rural Development Service Technical Advice Note 29. DEFRA, London, UK.Google Scholar
De Smet, S, Raes, K and Demeyer, D 2004. Meat fatty acid composition as affected by fatness and genetic factors: a review. Animal Research 53, 81–98.Google Scholar
Dewhurst, RJ, Scollan, ND, Youell, SJ, Tweed, JKS and Humphreys, MO 2001. Influence of species, cutting date and cutting interval on the fatty acid composition of grasses. Grass and Forage Science 56, 68–74.Google Scholar
Dewhurst, RJ, Shingfield, KJ, Lee, MRF and Scollan, ND 2006. Increasing the concentrations of beneficial polyunsaturated fatty acids in milk produced by dairy cows in high-forage systems. Animal Feed Science and Technology 131, 168–206.Google Scholar
Elgersma, A 2015. Grazing increases the unsaturated fatty acid concentration of milk from grass-fed cows: a review of the contributing factors, challenges and future perspectives. European Journal of Lipid Science and Technology 117, 1345–1369.Google Scholar
Enser, M, Hallett, K, Hewitt, B, Fursey, GAJ and Wood, JD 1996. Fatty acid content and composition of English beef, lamb and pork at retail. Meat Science 42, 443–456.Google Scholar
Folch, J, Lees, M and Stanley, GH 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.Google Scholar
Girard, M, Dohme-Meier, F, Silacci, P, Kragten, SA, Kreuzer, M and Bee, G 2016a. Forage legumes rich in condensed tannins may increase n-3 fatty acid levels and sensory quality of lamb meat. Journal of the Science of Food and Agriculture 96, 1923–1933.Google Scholar
Girard, M, Dohme-Meier, F, Wechsler, D, Goy, D, Kreuzer, M and Bee, G 2016b. Ability of 3 tanniferous forage legumes to modify quality of milk and Gruyere-type cheese. Journal of Dairy Science 99, 205–220.Google Scholar
Harfoot, CG 1981. Lipid metabolism in the rumen. In Lipid metabolism in ruminant animals (ed. WW Christie), pp. 21–55. Pergamon Press, Oxford, UK.Google Scholar
Hopkins, A, Pywell, RF, Peel, S, Johnson, RH and Bowling, PJ 1999. Enhancement of botanical diversity of permanent grassland and impact on hay production in Environmentally Sensitive Areas in the UK. Grass and Forage Science 54, 163–173.Google Scholar
Jouany, JP, Lassalas, B, Doreau, M and Glasser, F 2007. Dynamic features of the rumen metabolism of linoleic acid, linolenic acid and linseed oil measured in vitro. Lipids 42, 351–360.Google Scholar
Kliem, KE, Deaville, ER, Morgan, R and Givens, DI 2006. Do biodiverse pastures have the potential to improve the fatty acid profile of ruminant meat? In Proceedings of the 8th British Grassland Society Research Conference, Cirencester, UK, p. 81.Google Scholar
Kramer, JKG and Zhou, J 2001. Conjugated linoleic acid and octadecenoic acids: extraction and isolation of lipids. European Journal of Lipid Science and Technology 103, 594–600.Google Scholar
Kumarasamy, Y, Middleton, M, Reid, RG, Nahar, L and Sarker, SD 2003. Biological activity of serotonin conjugates from the seeds of Centaurea nigra . Fitoterapia 74, 609–612.Google Scholar
Lee, MRF, Theobald, VJ, Gordon, N, Leyland, M, Tweed, JKS, Fychan, R and Scollan, ND 2014. The effect of high polyphenol oxidase grass silage on metabolism of polyunsaturated fatty acids and nitrogen across the rumen of beef steers. Journal of Animal Science 92, 5076–5087.Google Scholar
Lourenço, M, Van Ranst, G, De Smet, S, Raes, K and Fievez, V 2007. Effect of grazing pastures with different botanical composition by lambs on rumen fatty acid metabolism and fatty acid pattern of longissimus muscle and subcutaneous fat. Animal 1, 537–545.Google Scholar
Luescher, A, Mueller-Harvey, I, Soussana, JF, Rees, RM and Peyraud, JL 2014. Potential of legume-based grassland-livestock systems in Europe: a review. Grass and Forage Science 69, 206–228.Google Scholar
MAFF 1986. The analysis of agricultural materials. Reference book 427. HMSO, London.Google Scholar
Min, BR, Barry, TN, Attwood, GT and McNabb, WC 2002. The effect of condensed tannins from Lotus corniculatus on the proteolytic activities and growth of rumen bacteria. Journal of Animal Science 80 (suppl. 1), 1602.Google Scholar
Ørskov, ER and McDonald, I 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science 92, 499–503.Google Scholar
Pywell, RF, Bullock, JM, Hopkins, A, Walker, KJ, Sparks, TH, Burke, MJW and Peel, S 2002. Restoration of species-rich grassland on arable land: assessing the limiting processes using a multi-site experiment. Journal of Applied Ecology 39, 294–309.Google Scholar
Scollan, ND, Choi, NJ, Kurt, E, Fisher, AV, Enser, M and Wood, JD 2001. Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. British Journal of Nutrition 85, 115–124.Google Scholar
Shingfield, KJ, Ahvenjärvi, S, Toivonen, V, Ärölä, A, Nurmela, KVV, Huhtanen, P and Griinari, JM 2003. Effect of dietary fish oil on biohydrogenation of fatty acids and milk fatty acid content in cows. Animal Science 77, 165–179.Google Scholar
Sukhija, PS and Palmquist, DL 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36, 1202–1206.Google Scholar
Theodorou, MK, Williams, BA, Dhanoa, MS, McAllan, AB and France, J 1994. A simple gas production method to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48, 185–197.Google Scholar
Van Ranst, G, Vandewalle, M, Gadeyne, F, De Riek, J and Fievez, V 2013. Lipid metabolism in mixtures of red clover (Trifolium repens) and perennial ryegrass (Lolium perenne) in lab scale silages and in vitro rumen incubations. Animal 7, 1454–1463.Google Scholar
Wachira, AM, Sinclair, LA, Wilkinson, RG, Enser, M, Wood, JD and Fisher, AV 2002. Effects of dietary fat source and breed on the carcass composition, n-3 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue. British Journal of Nutrition 88, 697–709.Google Scholar
Whittington, FM, Dunn, R, Nute, GR, Richardson, RI and Wood, JD 2006. Effect of pasture type on lamb product quality. In New developments in sheepmeat quality. 9th Annual Langford Food Industry Conference, University of Bristol, Bristol, UK, pp. 27–31.Google Scholar
Supplementary material: File

Kliem et al. supplementary material

Tables S1-S3

Download Kliem et al. supplementary material(File)
File 39.5 KB