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Effects of duodenal infusion of free α-linolenic acid on the plasma and milk proteome of lactating dairy cows

Published online by Cambridge University Press:  24 July 2012

Y. X. Yang ,
J. Q. Wang ,
T. J. Yuan ,
D. P. Bu ,
J. H. Yang ,
P. Sun  and
L. Y. Zhou
Y. X. Yang
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China
J. Q. Wang*
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
T. J. Yuan
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
D. P. Bu
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
J. H. Yang
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
P. Sun
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
L. Y. Zhou
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
*
†E-mail: jqwangcaas@gmail.com
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Abstract

This study is an exploratory analysis for understanding the effect of a duodenal infusion of an α-linolenic acid (LNA) on the plasma and milk proteome of lactating dairy cows. Four primiparous Holstein cows were fitted with duodenal cannulas and received 0, 100, 200, 300 and 400 g/day of LNA in a two-treatment crossover design. Blood and milk were collected for determination of protein composition by two-dimensional gel electrophoresis. Alteration of protein spots was detected and identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS). Plasma haptoglobin levels, and milk β-casein A2, αs1-casein variant and albumin, did not differ in cows after infusion of 0, 100, 200 and 300 g/day of LNA, but were increased after the cows received duodenal infusion of 400 g/day of LNA. Western blot analysis of haptoglobin expression in plasma confirmed the alterations in protein expression seen using MS. This study demonstrated that infusion of high doses of LNA by duodenal cannula can result in metabolic stress within the bovine intestine and in changes in milk composition.

Keywords

dairy cowlinolenic acidplasmamilkproteome
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 2 , February 2013 , pp. 293 - 299
Copyright
Copyright © The Animal Consortium 2012

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References

Aich, P, Jalal, S, Czuba, C, Schatte, G, Herzog, K, Olson, DJ, Ross, AR, Potter, AA, Babiuk, LA, Griebel, P 2007. Comparative approaches to the investigation of responses to stress and viral infection in cattle. OMICS 11, 413–434.CrossRefGoogle Scholar
Araujo, DB, Cooke, RF, Hansen, GR, Staples, CR, Arthington, JD 2010. Effects of rumen-protected polyunsaturated fatty acid supplementation on performance and physiological responses of growing cattle after transportation and feedlot entry. Journal of Animal Science 88, 4120–4132.CrossRefGoogle ScholarPubMed
Bionaz, M, Trevisi, E, Calamari, L, Librandi, F, Ferrari, A, Bertoni, G 2007. Plasma paraoxonase, health, inflammatory conditions, and liver function in transition dairy cows. Journal of Dairy Science 90, 1740–1750.Google Scholar
Boehmer, JL, Bannerman, DD, Shefcheck, K, Ward, JL 2008. Proteomic analysis of differentially expressed proteins in bovine milk during experimentally induced Escherichia coli mastitis. Journal of Dairy Science 91, 4206–4218.CrossRefGoogle ScholarPubMed
Boehmer, JL, Ward, JL, Peters, RR, Shefcheck, KJ, McFarland, MA, Bannerman, DD 2010. Proteomic analysis of the temporal expression of bovine milk proteins during coliform mastitis and label-free relative quantification. Journal of Dairy Science 93, 593–603.Google Scholar
Bu, DP, Wang, JQ, Dhiman, TR, Liu, SJ 2007. Effectiveness of oils rich in linoleic and linolenic acids to enhance conjugated linoleic acid in milk from dairy cows. Journal of Dairy Science 90, 998–1007.Google Scholar
Burdge, GC, Calder, PC 2006. Dietary alpha-linolenic acid and health-related outcomes: a metabolic perspective. Nutrition Research Reviews 19, 26–52.Google Scholar
Cairoli, F, Battocchio, M, Veronesi, MC, Brambilla, D, Conserva, F, Eberini, I, Wait, R, Gianazza, E 2006. Serum protein pattern during cow pregnancy: acute-phase proteins increase in the peripartum period. Electrophoresis 27, 1617–1625.Google Scholar
Candiano, G, Bruschi, M, Musante, L, Santucci, L, Ghiggeri, GM, Carnemolla, B, Orecchia, P, Zardi, L, Righetti, PG 2004. Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25, 1327–1333.CrossRefGoogle Scholar
Cooke, RF, Bohnert, DW 2011. Technical note: bovine acute-phase response after corticotrophin-release hormone challenge. Journal of Animal Science 89, 252–257.Google Scholar
Côrtes, C, Kazama, R, da Silva-Kazama, D, Benchaar, C, Zeoula, LM, Santos, GT, Petit, HV 2011. Digestion, milk production and milk fatty acid profile of dairy cows fed flax hulls and infused with flax oil in the abomasum. Journal of Dairy Research 78, 293–300.Google Scholar
Eckersall, PD, Young, FJ, McComb, C, Hogarth, CJ, Safi, S, Weber, A, McDonald, T, Nolan, AM, Fitzpatrick, JL 2001. Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Veterinary Record 148, 35–41.Google Scholar
Hogarth, CJ, Fitzpatrick, JL, Nolan, AM, Young, FJ, Pitt, A, Eckersall, PD 2004. Differential protein composition of bovine whey: a comparison of whey from healthy animals and from those with clinical mastitis. Proteomics 4, 2094–2100.Google Scholar
Ibeagha-Awemu, EM, Ibeagha, AE, Messier, S, Zhao, X 2010. Proteomics, genomics, and pathway analyses of Escherichia coli and Staphylococcus aureus infected milk whey reveal molecular pathways and networks involved in mastitis. Journal of Proteome Research 9, 4604–4619.Google Scholar
Khas-Erdene, Q, Wang, JQ, Bu, DP, Wang, L, Drackley, JK, Liu, QS, Yang, G, Wei, HY, Zhou, LY 2010. Short communication: responses to increasing amounts of free alpha-linolenic acid infused into the duodenum of lactating dairy cows. Journal of Dairy Science 93, 1677–1684.Google Scholar
Khiaosa-Ard, R, Klevenhusen, F, Soliva, CR, Kreuzer, M, Leiber, F 2010. Transfer of linoleic and linolenic acid from feed to milk in cows fed isoenergetic diets differing in proportion and origin of concentrates and roughages. Journal of Dairy Research 77, 331–336.Google Scholar
Larsen, LB, Hinz, K, Jorgensen, AL, Moller, HS, Wellnitz, O, Bruckmaier, RM, Kelly, AL 2010. Proteomic and peptidomic study of proteolysis in quarter milk after infusion with lipoteichoic acid from Staphylococcus aureus. Journal of Dairy Science 93, 5613–5626.Google Scholar
Litherland, NB, Thire, S, Beaulieu, AD, Reynolds, CK, Benson, JA, Drackley, JK 2005. Dry matter intake is decreased more by abomasal infusion of unsaturated free fatty acids than by unsaturated triglycerides. Journal of Dairy Science 88, 632–643.CrossRefGoogle ScholarPubMed
Liu, Q, Wang, J, Bu, D, Khas, E, Liu, K, Wei, H, Zhou, L, Beitz, DC 2010. Influence of linolenic acid content on the oxidation of milk fat. Journal of Agricultural and Food Chemistry 58, 3741–3746.Google Scholar
Mansson, HL 2008. Fatty acids in bovine milk fat. Food and Nutrition Research 52. doi:10.3402/fnr.v52i0.1821Google Scholar
Moyes, KM, Drackley, JK, Salak-Johnson, JL, Morin, DE, Hope, JC, Loor, JJ 2009. Dietary-induced negative energy balance has minimal effects on innate immunity during a Streptococcus uberis mastitis challenge in dairy cows during midlactation. Journal of Dairy Science 92, 4301–4316.Google Scholar
Napoli, A, Aiello, D, Di Donna, L, Prendushi, H, Sindona, G 2007. Exploitation of endogenous protease activity in raw mastitic milk by MALDI-TOF/TOF. Analytical Chemistry 79, 5941–5948.Google Scholar
Piccinini, R, Binda, E, Belotti, M, Casirani, G, Zecconi, A 2004. The evaluation of non-specific immune status of heifers in field conditions during the periparturient period. Veterinary Research 35, 539–550.CrossRefGoogle ScholarPubMed
Pyörälä, S 2003. Indicators of inflammation in the diagnosis of mastitis. Veterinary Research 34, 565–578.Google Scholar
Relling, AE, Reynolds, CK 2007. Feeding rumen-inert fats differing in their degree of saturation decreases intake and increases plasma concentrations of gut peptides in lactating dairy cows. Journal of Dairy Science 90, 1506–1515.Google Scholar
Shaikh, SR, Edidin, M 2007. Immunosuppressive effects of polyunsaturated fatty acids on antigen presentation by human leukocyte antigen class I molecules. The Journal of Lipid Research 48, 127–138.Google Scholar
Silvestre, FT, Carvalho, TS, Crawford, PC, Santos, JE, Staples, CR, Jenkins, T, Thatcher, WW 2011. Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins. Journal of Dairy Science 94, 2285–2301.Google Scholar
Somers, JM, O'Brien, B, Meaney, WJ, Kelly, AL 2003. Heterogeneity of proteolytic enzyme activities in milk samples of different somatic cell count. Journal of Dairy Research 70, 45–50.Google Scholar
Sun, P, Wang, J, Yang, G, Khas, E, Liu, Q 2010. Effects of different doses of free alpha-linolenic acid infused to the duodenum on the immune function of lactating dairy cows. Archives of Animal Nutrition 64, 504–513.Google Scholar
Vangroenweghe, F, Duchateau, L, Burvenich, C 2004. Moderate inflammatory reaction during experimental Escherichia coli mastitis in primiparous cows. Journal of Dairy Science 87, 886–895.Google Scholar
Ward, AT, Wittenberg, KM, Przybylski, R 2002. Bovine milk fatty acid profiles produced by feeding diets containing solin, flax and canola. Journal of Dairy Science 85, 1191–1196.CrossRefGoogle ScholarPubMed
Wedholm, A, Moller, HS, Lindmark-Mansson, H, Rasmussen, MD, Andren, A, Larsen, LB 2008. Identification of peptides in milk as a result of proteolysis at different levels of somatic cell counts using LC MALDI MS/MS detection. Journal of Dairy Research 75, 76–83.Google Scholar
Yamane, K, Minamoto, A, Yamashita, H, Takamura, H, Miyamoto-Myoken, Y, Yoshizato, K, Nabetani, T, Tsugita, A, Mishima, HK 2003. Proteome analysis of human vitreous proteins. Molecular and Cellular Proteomics 2, 1177–1187.Google Scholar
Yaqoob, P, Calder, PC 2007. Fatty acids and immune function: new insights into mechanisms. British Journal of Nutrition 98 (suppl. 1), S41–S45.Google Scholar
Zachut, M, Arieli, A, Lehrer, H, Livshitz, L, Yakoby, S, Moallem, U 2010. Effects of increased supplementation of n-3 fatty acids to transition dairy cows on performance and fatty acid profile in plasma, adipose tissue, and milk fat. Journal of Dairy Science 93, 5877–5889.Google Scholar