Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-10T10:58:24.713Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of herbage allowance of native grasslands in purebred and crossbred beef cows: metabolic, endocrine and hepatic gene expression profiles through the gestation–lactation cycle

Published online by Cambridge University Press:  12 May 2014

J. Laporta ,
A. L. Astessiano ,
C. López-Mazz ,
P. Soca ,
A. C. Espasandin  and
M. Carriquiry
J. Laporta
Affiliation:
Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Av. E. Garzón 780, 12900, Montevideo, Uruguay
A. L. Astessiano
Affiliation:
Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Av. E. Garzón 780, 12900, Montevideo, Uruguay
C. López-Mazz
Affiliation:
Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Av. E. Garzón 780, 12900, Montevideo, Uruguay
P. Soca
Affiliation:
Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Av. E. Garzón 780, 12900, Montevideo, Uruguay
A. C. Espasandin
Affiliation:
Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Av. E. Garzón 780, 12900, Montevideo, Uruguay
M. Carriquiry*
Affiliation:
Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Av. E. Garzón 780, 12900, Montevideo, Uruguay
*
†E-mail: mcarriquiry@fagro.edu.uy
Get access

Abstract

Our objective was to evaluate the metabolic, endocrine and hepatic mRNA profiles through the gestation–lactation cycle in purebred (PU: Angus and Hereford) and crossbred (CR: reciprocal F1 crosses) mutliparous beef cows (n=32), grazing on two herbage allowances of native pastures (2.5 v. 4 kg dry matter/kg BW; LO v. HI) and their associations with cow’s productive performance (calf birth weight, milk production and commencement of luteal activity). Cow BW, body condition score (BCS) and blood samples were collected monthly, starting at −165 days relative to calving (days), and every 2 weeks after calving until +60 days of lactation. Liver biopsies were collected at −165, −75, −45, −15±10, and +15 and +60±3 days. Metabolic, endocrine and hepatic gene expression profiles, and calf birth weight, milk yield and postpartum commencement of luteal activity were evaluated. Overall, the most pronounced changes in metabolic, endocrine and hepatic gene expression occurred during winter gestation (−165 to −45 days), when all cows experienced the onset of a negative energy balance (decreased BCS, glucose and insulin, and increased non-esterified fatty acid concentrations, P<0.008). Concentrations of insulin and IGF-I were greater (P<0.037) in HI than in LO cows. However, serum IGF-I concentrations and hepatic growth hormone receptor (GHR) and IGF1 mRNA decreased (P<0.05) during the winter gestation period only in HI cows. Although IGF-I concentrations decreased (P<0.05) during the early postpartum (−15 v.+15 days) for all cows, the typical molecular mechanism that control the uncoupling of the growth hormone-IGF1 axis during the transition period of the dairy cattle (reduced hepatic GHR1A and IGF-I mRNA) was not observed in this study. The hepatic mRNA expression of key transcripts involved in gluconeogenesis and fatty-acid oxidation were upregulated (P<0.05) during winter gestation (from −165 to −45, −15 or +15 days, depending on the cow groups). Particularly, acyl-CoA oxidase-1 mRNA was greater for CR than for PU cows during winter gestation (−75 and −45 days), and fibroblast growth factor-21 mRNA was downregulated (P<0.01) only for HI cows during the transition (−15 v. 15 days) and lactation period (+15 to +60 days, P<0.01). These results, together with the greater BCS, estimated energy intake, increased milk yield and shorter commencement of luteal activity in HI than in LO, and in CR than in PU cows (P<0.018), would indicate that HI and CR cows were able to adapt more efficiently to changes in nutrient and energy supply through the gestation–lactation cycle.

Keywords

beef cattlemRNAliverrangelandenergy balance
Type
Full Paper
Information
animal , Volume 8 , Issue 7 , July 2014 , pp. 1119 - 1129
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Astessiano, AL, Pérez-Clariget, R, Quintans, G, Soca, P and Carriquiry, M 2012. Effects of a short-term increase in the nutritional plane before the mating period on metabolic and endocrine parameters, hepatic gene expression and reproduction in primiparous beef cows on grazing conditions. Journal of Animal Physiology and Animal Nutrition 96, 535–544.Google Scholar
Baird, GD, Lomax, MA, Symonds, HW and Shaw, SR 1980. Net hepatic and splanchnic metabolism of lactate, pyruvate and propionate in dairy cows in vivo in relation to lactation and nutrient supply. Biochemestry Journal 186, 47–57.Google Scholar
Bauman, DE 2000. Regulation of nutrient partitioning during lactation: homeostasis and homeorhesis revisited. In Ruman physiology: digestion, metabolism and growth and growth and reproduction (ed. PJ Cronje), pp. 311–327. CAB Publishing, New York, NY, USA.Google Scholar
Bell, AW 1995. Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation. Journal of Animal Science 73, 2804–2819.CrossRefGoogle ScholarPubMed
Blanc, F, Broquier, F, Agabriel, J, D’Hour, P and Chiliard, Y 2006. Adaptive abilities of the females and sustainability of ruminant livestock systems. A review. Animal Research 55, 489–510.Google Scholar
Brosh, A 2007. Heart rate measurements as an index of energy expenditure and energy balance in ruminants: a review. Jornal of Animal Science 85, 1213–1227.Google Scholar
Carriquiry, M, Weber, WJ, Fahrenkrug, SC and Crooker, BA 2009. Hepatic gene expression in multiparous Holstein cows treated with bovine somatotropin and fed n-3 fatty acids in early lactation. Journal of Dairy Science 92, 4889–4900.Google Scholar
Chapman, DF, Parsons, AJ, Cosgrove, GP, Barker, DJ, Marotti, DM, Venning, KJ, Rutter, SM, Hill, J and Thompson, AN 2007. Impacts of spatial patterns in pasture on animal grazing behavior, intake, and performance. Crop Science 47, 399–415.Google Scholar
Cundiff, LV, Gregory, KE and Koch, RM 1974. Effects of heterosis on reproduction in Hereford, Angus and Shorthorn cattle. Journal of Animal Science 38, 711–727.Google Scholar
Drackley, JK, Overton, TR and Douglas, GN 2001. Adaptations of glucose and long-chain fatty acid metabolism in liver of dairy cows during the periparturient period. Journal of Dairy Science 84, 100–112.Google Scholar
Espasandin, AC, Ciria, M, Franco, JB, Pereyra, F and Gimeno, D 2010. Heterosis and productive and reproductive performance in Angus, Hereford and F1 reciprocal crossed cows on grazing systems of Uruguay. Proceedings XVI World Buiatrics Congress, Santiago, Chile, 14–18 November, abstract 862.Google Scholar
Estall, JL, Ruasa, JL, Choib, CS, Laznika, D, Badmanc, M, Maratos-Flierc, E, Shulmanb, GI and Spiegelmana, BM 2009. PGC-1α negatively regulates hepatic FGF21 expression by modulating the heme/Rev-Erb axis. Proceedings of the National Academy of Science 52, 22510–22515.Google Scholar
Grum, DE, Hansen, LR and Drackley, JK 1994. Peroxisomal β-oxidation of fatty acids in bovine and rat liver. Comparative Biochemestry and Physiology 109, 281–292.Google Scholar
Haydock, KP and Shaw, NH 1975. The comparative yield method for estimating dry matter yield of pasture. Australian Journal of Experimental Agriculture and Animal Husbandry 15, 663–670.Google Scholar
Ingvartsen, KL, Dewhurst, RJ and Friggens, NC 2003. On the relationship between lactational performance and health: is it yield or metabolic imbalance that cause production diseases in dairy cattle? A position paper. Livestock Production Science 83, 277–308.Google Scholar
Jiang, H, Lucy, MC, Crooker, BA and Beal, WE 2005. Expression of growth hormone receptor 1A mRNA is decreased in dairy cows but not in beef cows at parturition. Journal of Dairy Science 88, 1370–1377.Google Scholar
Jorritsma, R, Wensinga, T, Kruipb, TAM, Vosa, PLAM and Noordhuizena, JPTM 2003. Metabolic changes in early lactation and impaired reproductive performance in dairy cows. Veterinary Research 34, 11–26.CrossRefGoogle ScholarPubMed
Kersten, S 2002. Peroxisome proliferator activated receptors and obesity. European Journal of Pharmacology 440, 223–234.Google Scholar
Kliewer, SA and Mangelsdorf, DJ 2010. Fibroblast growth factor 21: from pharmacology to physiology. The American Journal of Clinical Nutrition 91, 254S–257S.Google Scholar
Loor, JJ 2010. Genomics of metabolic adaptations in the peripartal cow. Animal 4, 1110–1139.Google Scholar
Loor, JJ, Dann, HM, Everts, RE, Oliveira, R, Green, CA, Janovick Guretzky, NA, Rodriguez-Zas, SL, Lewin, HA and Drackley, JK 2005. Temporal gene expression profiling of liver from periparturient dairy cows reveals complex adaptive mechanisms in hepatic function. Physiological Genomics 23, 217–226.Google Scholar
Lucy, MC 2008. Functional differences in the growth hormone and insulin-like growth factor axis in cattle and pigs: implications for post-partum nutrition and reproduction. Reproduction Domestic Animal 43, 31–39.Google Scholar
Lucy, MC, Verkerk, GA, Whyte, BE, MacDonald, KA, Burton, L, Cursons, RT, Roche, JR and Holmes, CW 2009. Somatotropic axis components and nutrient partitioning in genetically diverse dairy cows managed under different feed allowances in a pasture system. Journal of Dairy Science 92, 526–539.Google Scholar
Mashek, DG, Ingvartsen, KL, Andersen, JB, Vestergaard, M and Larsen, T 2001. Effects of a four-day hyperinsulinemic-euglycemic clamp in early and mid-lactation dairy cows on plasma concentrations of metabolites, hormones, and binding proteins. Domestic Animal Endocrinology 21, 169–185.Google Scholar
McCarthy, SD, Waters, SM, Kenny, DA, Diskin, MG, Fitzpatrick, R, Patton, J, Wathes, DC and Morris, DG 2010. Negative energy balance and hepatic gene expression patterns in high-yielding dairy cows during the early postpartum period: a global approach. Physiological Genomics 42, 188–199.Google Scholar
Minick, JA, Buchanan, DS and Rupert, SD 2001. Milk production of crossbred daughters of high- and low-milk EPD Angus and Hereford bulls. Journal of Animal Science 79, 1386–1393.CrossRefGoogle ScholarPubMed
Morris, CA, Baker, RL, Johnson, DL, Carter, AH and Hunter, JC 1987. Reciprocal crossbreeding of Angus and Hereford cattle. 3. Cow weight, reproduction, maternal performance, and lifetime production. New Zealand Journal of Agriculural Research 30, 453–467.Google Scholar
Mott, GO 1960. Grazing pressure and the measurement of pasture production. Proceedings 8th International Grassland Congress, Reading, UK, 11–21 July, pp. 606–611.Google Scholar
National Research Council (NRC). 2000. Nutrient requirements of beef cattle, 7th edition. National Academy Press, Washington, DC, USA.Google Scholar
Quintans, G, Banchero, G, Carriquiry, M, López, C and Baldi, F 2010. Effect of body condition and suckling restriction with and without presence of the calf on cow and calf performance. Animal Production Science 50, 931–938.Google Scholar
Rajaram, S, Baylink, DJ and Mohan, S 1997. Insulin-like growth factor-binding proteins in serum and other biological fluids: regulation and functions. Endocrine Reviews 18, 801–831.Google ScholarPubMed
Roberts, AJ, Nugent, RA, Klindt, J and Jenkins, TG 1997. Circulating insulin-like growth factor-I, insulin-like growth factor binding proteins, growth hormone, and resumption of estrus in postpartum cows subjected to dietary energy restriction. Journal of Animal Science 75, 1909–1917.Google Scholar
Soares, AB, Carvalho, PCF, Nabinger, C, Frizzo, A, Pinto, CE, Junior, JAF, Semmelmann, C and da Trindade, J 2003. Effect of changing herbage allowance on primary and secondary production of natural pasture. Proceedings of the 7th International Rangeland Congress, 26 July–1 August, pp. 966–968.Google Scholar
Schneider, A, Machado Pfeifer, LF, Teixeira Hax, L, Paludo, GR, Burkert Del Pino, FA, Laurino Dionello, NJ and Corrêa, MN 2010. Insulin-like growth factor and growth hormone receptor in postpartum lactating beef cows. Pesquisa Agropecuária Brasileira 45, 925–931.Google Scholar
Schoenberg, KM, Giesy, SL, Harvatine, KJ, Waldron, MR, Cheng, C, Kharitonenkov, A and Boisclair, YR 2011. Plasma FGF21 is elevated by the intense lipid mobilization of lactation. Endocrinology 152, 4652–4661.Google Scholar
Smit, HJ, Taweel, HZ, Tas, BM, Tamminga, S and Elgersma, A 2005. Comparison of techniques for estimating herbage intake of grazing dairy cows. Journal of Dairy Science 88, 1827–1836.CrossRefGoogle ScholarPubMed
Sollenberger, LE, Moore, JE, Allen, VG and Pedreira, CGS 2005. Reporting forage allowance in grazing experiments. Crop Science 45, 896–900.Google Scholar
Wang, Y, Eleswarapu, S, Beal, WE, Swecker, WS, Akers, RM and Jiang, H 2003. Reduced serum insulin-like growth factor (IGF) I is associated with reduced liver IGF-I mRNA and liver growth hormone receptor mRNA in food-deprived cattle. The Journal of Nutrition 133, 2555–2560.Google Scholar
Supplementary material: File

Laporta supplementary material

Laporta supplementary material

Download Laporta supplementary material(File)
File 28.2 KB
Supplementary material: File

Laporta Supplementary Material

Tables

Download Laporta Supplementary Material(File)
File 67.1 KB