Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-14T23:08:24.344Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of intravenous arginine infusion on inflammation and metabolic indices of dairy cows in early lactation

Published online by Cambridge University Press:  01 October 2019

L. Y. Ding Open the ORCID record for L. Y. Ding [Opens in a new window] ,
Y. F. Wang ,
Y. Z. Shen ,
G. Zhou ,
T. Y. Wu ,
X. Zhang ,
M. Z. Wang ,
J. J. Loor  and
J. Zhang
L. Y. Ding
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China Faculty of Science, School of Agriculture and Environment, The University of Western Australia, 35 Stirling Highway, Perth 6009, WA, Australia
Y. F. Wang
Affiliation:
Faculty of Physiology, Clinical Medical School, Southeast University, 87 Dingjiaqiao Road, Nanjing 210009, Jiangsu, P.R. China
Y. Z. Shen
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
G. Zhou
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
T. Y. Wu
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
X. Zhang
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
M. Z. Wang*
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
J. J. Loor
Affiliation:
Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana 61801, IL, USA
J. Zhang
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
*
†E-mail: mengzhiwang@126.com
Get access

Abstract

Enhancing the supply of arginine (Arg), a semi-essential amino acid, has positive effects on immune function in dairy cattle experiencing metabolic stress during early lactation. Our objective was to determine the effects of Arg supplementation on biomarkers of liver damage and inflammation in cows during early lactation. Six Chinese Holstein lactating cows with similar BW (508 ± 14 kg), body condition score (3.0), parity (4.0 ± 0), milk yield (30.6 ± 1.8 kg) and days in milk (20 ± days) were randomly assigned to three treatments in a replicated 3 × 3 Latin square design balanced for carryover effects. Each period was 21 days with 7 days for infusion and 14 days for washout. Treatments were (1) Control: saline; (2) Arg group: saline + 0.216 mol/day l-Arg; and (3) Alanine (Ala) group: saline + 0.868 mol/day l-Ala (iso-nitrogenous to the Arg group). Blood and milk samples from the experimental cows were collected on the last day of each infusion period and analyzed for indices of liver damage and inflammation, and the count and composition of somatic cells in milk. Compared with the Control, the infusion of Arg led to greater concentrations of total protein, immunoglobulin M and high density lipoprotein cholesterol coupled with lower concentrations of haptoglobin and tumor necrosis factor-α, and activity of aspartate aminotransferase in serum. Infusion of Ala had no effect on those biomarkers compared with the Control. Although milk somatic cell count was not affected, the concentration of granulocytes was lower in response to Arg infusion compared with the Control or Ala group. Overall, the biomarker analyses indicated that the supplementation of Arg via the jugular vein during early lactation alleviated inflammation and metabolic stress.

Keywords

amino acidshealthsomatic cell compositionmetabolic stressimmune function
Type
Research Article
Information
animal , Volume 14 , Issue 2 , February 2020 , pp. 346 - 352
Copyright
© The Animal Consortium 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

a

Present address: Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana 61801, IL, USA

References

Abdeltawwab, M, H. Ahmad, M, Khattab, Y and Shalaby, A 2010. Effect of dietary protein level, initial body weight, and their interaction on the growth, feed utilization, and physiological alterations of Nile tilapia, Oreochromis niloticus (L.). Aquaculture 298, 267–274.CrossRefGoogle Scholar
Auldist, MJ and Hubble, IB 1998. Effects of mastitis on raw milk and dairy products. Australian Journal of Dairy Technology 53, 28–36.Google Scholar
Batistel, F, Arroyo, J, Garces, CIM, Trevisi, E, Parys, C, Ballou, M, Cardoso, FC and Loor, J 2018. Ethyl-cellulose rumen-protected methionine alleviates inflammation and oxidative stress and improves neutrophil function during the periparturient period and early lactation in Holstein dairy cows. Journal of Dairy Science 101, 480–490.CrossRefGoogle ScholarPubMed
Bertoni, G, Minuti, A and Trevisi, E 2015. Immune system, inflammation and nutrition in dairy cattle. Animal Production Science 55, 943–948.CrossRefGoogle Scholar
Bionaz, M, Trevisi, E, Calamari, L, Librandi, F, Ferrari, A and Bertoni, G 2007. Plasma paraoxonase, health, inflammatory conditions, and liver function in transition dairy cows. Journal of Dairy Science 90, 1740–1750.CrossRefGoogle ScholarPubMed
Bradford, BJ, Mamedova, LK, Minton, JE, Drouillard, JS and Johnson, BJ 2009. Daily injection of tumor necrosis factor-α increases hepatic triglycerides and alters transcript abundance of metabolic genes in lactating dairy cattle. The Journal of Nutrition 139, 1451–1456.CrossRefGoogle ScholarPubMed
Cao, W, Xiao, L, Liu, G, Fang, T, Wu, X, Jia, G, Zhao, H, Chen, X, Wu, C and Cai, J 2016. Dietary arginine and N-carbamylglutamate supplementation enhances the antioxidant statuses of the liver and plasma against oxidative stress in rats. Food & Function 7, 2303–2311.CrossRefGoogle ScholarPubMed
Ding, L, Chen, L, Wang, M, Zhang, J, Loor, J, Zhou, G, Zhang, X and Wang, H 2018. Inhibition of arginase via jugular infusion of Nω-hydroxy-nor-l-arginine inhibits casein synthesis in lactating dairy cows. Journal of Dairy Science 101, 3514–3523.CrossRefGoogle ScholarPubMed
Ding, L, Shen, Y, Wang, Y, Zhou, G, Zhang, X, Wang, M, Loor, JJ, Chen, L and Zhang, J 2019a. Jugular arginine supplementation increases lactation performance and nitrogen utilization efficiency in lactating dairy cows. Journal of Animal Science and Biotechnology 10, 3.CrossRefGoogle ScholarPubMed
Ding, L, Wang, Y, Shen, Y, Zhou, G, Zhang, X, Wang, M, Loor, J and Zhang, J 2019b. Effects of arginase inhibition via jugular infusion of Nω-hydroxy-nor-l-arginine on metabolic and immune indices in lactating dairy cows. Journal of Dairy Science 102, 3310–3320.CrossRefGoogle ScholarPubMed
Favero, SD, Roschel, H, Solis, MY, Hayashi, AP, Artioli, GG, Otaduy, MC, Benatti, FB, Harris, RC, Wise, JA and Leite, CC 2012. Beta-alanine (Carnosyn™) supplementation in elderly subjects (60–80 years): effects on muscle carnosine content and physical capacity. Amino Acids 43, 49–56.CrossRefGoogle ScholarPubMed
Field, CJ, Johnson, IR and Schley, PD 2002. Nutrients and their role in host resistance to infection. Journal of Leukocyte Biology 71, 16–32.Google ScholarPubMed
Grossi, P, Bertoni, G, Cappelli, FP and Trevisi, E 2013. Effects of the precalving administration of omega-3 fatty acids alone or in combination with acetylsalicylic acid in periparturient dairy cows. Journal of Animal Science 91, 2657–2666.CrossRefGoogle ScholarPubMed
Grummer, RR 2008. Nutritional and management strategies for the prevention of fatty liver in dairy cattle. Veterinary Journal 176, 10–20.CrossRefGoogle ScholarPubMed
Guzik, TJ, Korbut, R and Adamek-Guzik, T 2003. Nitric oxide and superoxide in inflammation and immune regulation. Journal of Physiology & Pharmacology 54, 469–487.Google ScholarPubMed
Hu, S, Li, X, Rezaei, R, Meininger, CJ, Mcneal, CJ and Wu, G 2015. Safety of long-term dietary supplementation with L-arginine in pigs. Amino Acids 47, 925–936.CrossRefGoogle ScholarPubMed
Jobgen, WS, Fried, SK, Fu, WJ, Meininger, CJ and Wu, G 2006. Regulatory role for the arginine–nitric oxide pathway in metabolism of energy substrates. Journal of Nutritional Biochemistry 17, 571–588.CrossRefGoogle ScholarPubMed
Jr, KP 2000. The metabolic pathways of high-density lipoprotein, low-density lipoprotein, and triglycerides: a current review. American Journal of Cardiology 86, 5–10.Google Scholar
Kahl, S, Elsasser, TH and Blum, JW 1997. Nutritional regulation of plasma tumor necrosis factor-alpha and plasma and urinary nitrite/nitrate responses to endotoxin in cattle. Society for Experimental Biology and Medicine 215, 370–376.CrossRefGoogle ScholarPubMed
Koess, C and Hamann, J 2008. Detection of mastitis in the bovine mammary gland by flow cytometry at early stages. Journal of Dairy Research 75, 225–232.CrossRefGoogle ScholarPubMed
Latimer, KS and Duncan, JR 2011. Duncan and prasse's veterinary laboratory medicine: clinical pathology (5th ed). John Wiley & Sons Incorporated, Press, Hoboken, US.Google Scholar
Leitner, G, Merin, U and Silanikove, N 2011. Effects of glandular bacterial infection and stage of lactation on milk clotting parameters: comparison among cows, goats and sheep. International Dairy Journal 21, 279–285.CrossRefGoogle Scholar
Li, Q, Liu, Y, Che, Z, Zhu, H, Meng, G, Hou, Y, Ding, B, Yin, Y and Chen, F 2012. Dietary L-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs. Innate Immunity 18, 804–814.CrossRefGoogle ScholarPubMed
Li, N, Richoux, R, Perruchot, MH, Boutinaud, M, Mayol, JF and Gagnaire, V 2015. Flow cytometry approach to quantify the viability of milk somatic cell counts after various physico-chemical treatments. PLoS ONE 10, e0146071.CrossRefGoogle ScholarPubMed
Li, P, Yin, YL, Li, D, Kim, SW and Wu, S 2007. Amino acids and immune function. British Journal of Nutrition 98, 237–252.CrossRefGoogle ScholarPubMed
Loor, JJ, Massimo, B and Drackley, JK 2013. Systems physiology in dairy cattle: nutritional genomics and beyond. Annual Review of Animal Bioscience 1, 365–392.CrossRefGoogle ScholarPubMed
Malavé, I and Layrisse, M 1976. Immune response in malnutrition. Differential effect of dietary protein restriction on the IgM and IgG response to alloantigens. Cellular Immunology 21, 337–343.CrossRefGoogle ScholarPubMed
Marija, K, Arie, H and Jeffrey, B 2011. Body condition scoring of dairy cows. University of Ljuljana Press, Ljuljana, Slovenia.Google Scholar
Méndez, JD and Balderas, F 2001. Regulation of hyperglycemia and dyslipidemia by exogenous L-arginine in diabetic rats. Biochimie 83, 453–458.CrossRefGoogle ScholarPubMed
Moinard, C, Cynober, L and Bandt, JPD 2005. Polyamines: metabolism and implications in human diseases. Clinical Nutrition 24, 184–197.CrossRefGoogle ScholarPubMed
Nikolic, J, Stojanovic, I and Pavlovic, R 2007. The role of L-arginine in toxic liver failure: interrelation of arginase, polyamine catabolic enzymes and nitric oxide synthase. Amino Acids 32, 127–131.CrossRefGoogle ScholarPubMed
Piepers, S, De Vliegher, S, Demeyere, K, Lambrecht, BN, de Kruif, A, Meyer, E and Opsomer, G 2009. Technical note: flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability. Journal of Dairy Science 92, 626–631.CrossRefGoogle ScholarPubMed
Ren, W, Jie, Y, Wu, M, Gang, L, Guan, Y, Yan, X, Su, D, Li, W, Li, T and Shuai, C 2014. Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis. PLoS ONE 9, e88335.CrossRefGoogle ScholarPubMed
Sordillo, LM and Aitken, SL 2009. Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary Immunology & Immunopathology 128, 104–109.CrossRefGoogle ScholarPubMed
Strzałkowska, N, Józ´Wik, A, Bagnicka, E, KrzyżEwski, J and Horban´Czuk, JO 2009. Studies upon genetic and environmental factors affecting the cholesterol content of cow milk. I. Relationship between the polymorphic form of beta-lactoglobulin, somatic cell count, cow age and stage of lactation and cholesterol content of milk. Animal Science Papers & Reports 27, 95–103.Google Scholar
Tan, B, Li, XG, Kong, XF, Huang, RL, Zheng, R, Yao, K, Deng, ZY, Xie, MY, Shinzato, I and Yin, YL 2009. Dietary L-arginine supplementation enhances the immune status in early-weaned piglets. Amino Acids 37, 323–331.CrossRefGoogle ScholarPubMed
Tan, J, Liu, S, Guo, Y, Applegate, TJ and Eicher, SD 2014. Dietary l-arginine supplementation attenuates lipopolysaccharide-induced inflammatory response in broiler chickens. British Journal of Nutrition 111, 1394–1404.CrossRefGoogle ScholarPubMed
Trevisi, E, Moscati, L and Amadori, M 2016. Disease-predicting and prognostic potential of innate immune responses to noninfectious stressors: human and animal models. Elsevier Inc. Press, London, UK.Google Scholar
Vishal, B and Ochoa, JB 2003. Arginine availability, arginase, and the immune response. Current Opinion in Clinical Nutrition & Metabolic Care 6, 223–228.Google Scholar
Wang, M, Xu, B, Wang, H, Bu, D, Wang, J and Loor, JJ 2014. Effects of arginine concentration on the in vitro expression of Casein and mTOR pathway related genes in mammary epithelial cells from dairy cattle. PLoS ONE 9, e95985.CrossRefGoogle ScholarPubMed
Williams, E 1949. Experimental designs balanced for the estimation of residual effects of treatments. Australian Journal of Scientific Research 2, 149–168.Google Scholar
Zhao, FF, Wu, TY, Wang, HR, Ding, LY, Ahmed, G, Li, HW, Tian, W and Shen, YZ 2018. Jugular arginine infusion relieves lipopolysaccharide-triggered inflammatory stress and improves immunity status of lactating dairy cows. Journal of Dairy Science 101, 5961–5970.CrossRefGoogle ScholarPubMed