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Effects of micronutrient supplementation on performance and epigenetic status in dairy cows

Published online by Cambridge University Press:  11 June 2020

M. Gasselin ,
M. Boutinaud ,
A. Prézelin ,
P. Debournoux ,
M. Fargetton ,
E. Mariani ,
J. Zawadzki ,
H. Kiefer  and
H. Jammes Open the ORCID record for H. Jammes [Opens in a new window]
M. Gasselin
Affiliation:
Université Paris-Saclay, UVSQ, INRAE, BREED, 78350Jouy-en-Josas, France Ecole Nationale Vétérinaire d’Alfort, BREED, 94700Maisons-Alfort, France
M. Boutinaud
Affiliation:
INRAE, AGROCAMPUS Ouest PEGASE, 35590Saint-Gilles, France
A. Prézelin
Affiliation:
Université Paris-Saclay, UVSQ, INRAE, BREED, 78350Jouy-en-Josas, France Ecole Nationale Vétérinaire d’Alfort, BREED, 94700Maisons-Alfort, France
P. Debournoux
Affiliation:
INRAE, AGROCAMPUS Ouest PEGASE, 35590Saint-Gilles, France
M. Fargetton
Affiliation:
INRAE, AGROCAMPUS Ouest PEGASE, 35590Saint-Gilles, France
E. Mariani
Affiliation:
XR-Repro, 43700Coubon, France
J. Zawadzki
Affiliation:
Pilardière Group®, 85590Saint-Mars-la-Réorthe, France
H. Kiefer
Affiliation:
Université Paris-Saclay, UVSQ, INRAE, BREED, 78350Jouy-en-Josas, France Ecole Nationale Vétérinaire d’Alfort, BREED, 94700Maisons-Alfort, France
H. Jammes*
Affiliation:
Université Paris-Saclay, UVSQ, INRAE, BREED, 78350Jouy-en-Josas, France Ecole Nationale Vétérinaire d’Alfort, BREED, 94700Maisons-Alfort, France
*
†E-mail: helene.jammes@inrae.fr
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Abstract

The postpartum period is crucial in dairy cows and is marked by major physiological and metabolic changes that affect milk production, immune response and fertility. Nutrition remains the most important lever for limiting the negative energy balance and its consequences on general health status in highly selected dairy cows. In order to analyze the effect of a commercial micronutrient on intrinsic parameters, performances and the epigenome of dairy cows, 2 groups of 12 Holstein cows were used: 1 fed a standard diet (mainly composed of corn silage, soybean meal and non-mineral supplement) and the other 1 fed the same diet supplemented with the commercial micronutrient (µ-nutrient supplementation) for 4 weeks before calving and 8 weeks thereafter. Milk production and composition, BW, body condition score (BCS), DM intake (DMI) and health (calving score, metritis and mastitis) were recorded over the study period. Milk samples were collected on D15 and D60 post-calving for analyses of casein, Na+ and K+ contents and metalloprotease activity. Milk leukocytes and milk mammary epithelial cells (mMECs) were purified and counted. The viability of mMECs was assessed, together with their activity, through an analysis of gene expression. At the same time points, peripheral blood mononuclear cells (PBMCs) were purified and counted. Using genomic DNA extracted from PBMCs, mMECs and milk leukocytes, we assessed global DNA methylation (Me-CCGG) to evaluate the epigenetic imprinting associated with the µ-nutrient-supplemented diet. The µ-nutrient supplementation increased BCS and BW without modifying DMI or milk yield and composition. It also improved calving condition, reducing the time interval between calving and first service. Each easily collectable cell type displayed a specific pattern of Me-CCGG with only subtle changes associated with lactation stages in PBMCs. In conclusion, the response to the µ-nutrient supplementation improved the body condition without alteration of global epigenetic status in dairy cows.

Keywords

cowsperipartummicronutrientsmethylomeperformances
Type
Research Article
Information
animal , Volume 14 , Issue 11 , November 2020 , pp. 2326 - 2335
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of The Animal Consortium

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