Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-11T10:44:05.917Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of the processing methods of corn grain and soybean meal on milk protein expression profiles in dairy cows

Published online by Cambridge University Press:  17 September 2014

S. S. Li ,
J. S. Shen ,
D. X. Ren  and
J. X. Liu
S. S. Li
Affiliation:
Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang 310058, P. R. China
J. S. Shen
Affiliation:
Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang 310058, P. R. China
D. X. Ren
Affiliation:
Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang 310058, P. R. China
J. X. Liu*
Affiliation:
Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang 310058, P. R. China
*
†E-mail: liujx@zju.edu.reccn
Get access

Abstract

A proteomic approach was used to investigate the effects of the processing method of corn grain and soybean meal on the milk protein expression profile in lactating dairy cows. A total of 12 multiparous Holstein dairy cows were used in a 4×4 Latin square design with a 2×2 factorial arrangement. The primary factors examined were corn (finely ground (FGC) v. steam-flaked (SFC)) and soybean meal (solvent-extracted (SSBM) v. heat-treated (HSBM)), which were used to formulate four diets with the same basal ingredient: 27% FGC and 9% SSBM; 27% SFC and 9% SSBM; 27% FGC and 9% HSBM; and 27% SFC and 9% HSBM. Each period lasted for 21 days. Milk samples were collected on days 18, 19 and 20 of each period. Changes in the milk proteins were assessed by two-dimensional (2D) electrophoresis and ImageMaster 2D Platinum 6.0 software. A total of 13 spots displayed variations in protein spot abundance according to the statistical analysis. These spots were identified by a matrix-assisted laser desorption/ionization-time of flight/time of flight MS. According to the gels, the relative abundance of αs2-casein (CN) fragments was higher in the cows fed the SFC-HSBM than that for SFC-SSBM, whereas β-CN, α-lactalbumin and zinc-alpha-2-glycoprotein fragments were down-regulated in HSBM-fed cows. The relative decrease of β-CN expression was validated by western blot and agreed with the MS data. These results suggested that the method used to process soybean meal modified the synthesis and secretion of milk proteins in lactating dairy cows’ mammary glands.

Keywords

corn grainsoybean mealmilk proteomedairy cow
Type
Research Article
Information
animal , Volume 9 , Issue 2 , February 2015 , pp. 267 - 274
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

a

These two authors contributed equally to this work.

References

Bendixen, E, Danielsen, M, Hollung, K, Gianazza, E and Miller, I 2011. Farm animal proteomics–a review. Journal of Proteomics 74, 282–293.Google Scholar
Boehmer, JL, Bannerman, DD, Shefcheck, K and Ward, JL 2008. Proteomic analysis of differentially expressed proteins in bovine milk during experimentally induced Escherichia coli mastitis. Journal of Dairy Science 91, 4206–4218.Google Scholar
Burgi, W and Schmid, K 1961. Preparation and properties of Zn-alpha 2-glycoprotein of normal human plasma. The Journal of Biological Chemistry 236, 1066–1074.Google Scholar
Cabrita, ARJ, Dewhurst, RJ, Abreu, JMF and Fonseca, AJM 2006. Evaluation of the effects of synchronising the availability of N and energy on rumen function and production responses of dairy cows – a review. Animal Research 55, 1–24.CrossRefGoogle Scholar
Candiano, G, Bruschi, M, Musante, L, Santucci, L, Ghiggeri, GM, Carnemolla, B, Orecchia, P, Zardi, L and Righetti, PG 2004. Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25, 1327–1333.Google Scholar
Caroli, AM, Chessa, S and Erhardt, GJ 2009. Invited review: milk protein polymorphisms in cattle: effect on animal breeding and human nutrition. Journal of Dairy Science 92, 5335–5352.Google Scholar
Casper, DP, Maiga, HA, Brouk, MJ and Schingoethe, DJ 1999. Synchronization of carbohydrate and protein sources on fermentation and passage rates in dairy cows. Journal of Dairy Science 82, 1779–1790.CrossRefGoogle ScholarPubMed
Clark, JH, Klusmeyer, TH and Cameron, MR 1992. Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. Journal of Dairy Science 75, 2304–2323.Google Scholar
Cole, NA and Todd, RW 2008. Opportunities to enhance performance and efficiency through nutrient synchrony in concentrate-fed ruminants. Journal of Animal Science 86, E318–E333.Google Scholar
D’Amato, A, Bachi, A, Fasoli, E, Boschetti, E, Peltre, G, Senechal, H and Righetti, PG 2009. In-depth exploration of cow’s whey proteome via combinatorial peptide ligand libraries. Journal of Proteome Research 8, 3925–3936.Google Scholar
Daniels, KM, Webb, KE Jr, McGilliard, ML, Meyer, MJ, Van Amburgh, ME and Akers, RM 2006. Effects of body weight and nutrition on mammary protein expression profile in Holstein heifers. Journal of Dairy Science 89, 4276–4288.Google Scholar
Dewhurst, RJ, Davies, DR and Merry, RJ 2000. Microbial protein supply from the rumen. Animal Feed Science and Technology 85, 1–21.Google Scholar
Dziuba, J, Minkiewicz, P, Darewicz, M and Dziuba, B 2010. Milk proteins. In Handbook of dairy foods analysis (ed. LML Nollet and F Toldra), pp. 80–88. CRC press, Boca Raton, FL, USA.Google Scholar
Fox, PF and Brodkorb, A 2008. The CN micelle: historical aspects, current concepts and significance. International Dairy Journal 18, 677–684.Google Scholar
Galvani, M, Hamdan, M and Righetti, PG 2001. Two-dimensional gel electrophoresis/matrix-assisted laser desorption/ionization mass spectrometry of commercial bovine milk. Rapid Communications in Mass Spectrometry 15, 258–264.Google Scholar
Goncalves Lda, R, Soares, MR, Nogueira, FC, Garcia, C, Camisasca, DR, Domont, G, Feitosa, AC, Pereira Dde, A, Zingali, RB and Alves, G 2010. Comparative proteomic analysis of whole saliva from chronic periodontitis patients. Journal of Proteomics 73, 1334–1341.CrossRefGoogle ScholarPubMed
Gordon, WG, Semmett, WF, Cable, RS and Morris, M 1949. Amino acid composition of α-casein and β-casein. Journal of American Chemical Society 71, 3293–3297.Google Scholar
Herrera-Saldana, R and Huber, JT 1989. Influence of varying protein and starch degradabilities on performance of lactating cows. Journal of Dairy Science 72, 1477–1483.Google Scholar
Herrera-Saldana, R, Gomez-Alarcon, R, Torabi, M and Huber, JT 1990. Influence of synchronizing protein and starch degradation in the rumen on nutrient utilization and microbial protein synthesis. Journal of Dairy Science 73, 142–148.Google Scholar
Hirai, K, Hussey, HJ, Barber, MD, Price, SA and Tisdale, MJ 1998. Biological evaluation of a lipid-mobilizing factor isolated from the urine of cancer patients. Cancer Research 58, 2359–2365.Google Scholar
Holland, JW, Deeth, HC and Alewood, PF 2006. Resolution and characterisation of multiple isoforms of bovine kappa-casein by 2-DE following a reversible cysteine-tagging enrichment strategy. Proteomics 6, 3087–3095.Google Scholar
Huppertz, T 2013. Chemistry of the caseins. In Advanced dairy chemistry (ed. PLH McSweeney and PF Fox), pp. 135–160. Springer US, New York, NY, USA.Google Scholar
Kishore, A, Mukesh, M, Sobti, RC, Mishra, BP and Sodhi, M 2013. Variations in the regulatory region of alpha s1-casein milk protein gene among tropically adapted Indian native (Bos Indicus) cattle. ISRN Biotechnology 2013, 1–10.Google Scholar
Lu, LM, Li, QZ, Huang, JG and Gao, XJ 2013. Proteomic and functional analyses reveal MAPK1 regulates milk protein synthesis. Molecules 18, 263–275.Google Scholar
Ministry of Agriculture of P.R. China 2004. Feeding standard of dairy cattle (NY/T 34-2004). Ministry of Agriculture of P.R. China, Beijing, China.Google Scholar
O’Donnell, R, Holland, JW, Deeth, HC and Alewood, P 2004. Milk proteomics. International Dairy Journal 14, 1013–1023.CrossRefGoogle Scholar
Prizant, RL and Barash, I 2008. Negative effects of the amino acids Lys, His, and Thr on S6K1 phosphorylation in mammary epithelial cells. Journal of Cellular Biochemistry 105, 1038–1047.CrossRefGoogle ScholarPubMed
Reinhardt, TA and Lippolis, JD 2006. Bovine milk fat globule membrane proteome. Journal of Dairy Research 73, 406–416.Google Scholar
Sanchez, LM, Chirino, AJ and Bjorkman, P 1999. Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules. Science 283, 1914–1919.Google Scholar
SAS Institute 2000. SAS user’s guide: statistics.Version 8.01. SAS Institute Incorporation, Cary, NC, USA.Google Scholar
Schingoethe, DJ 1996. Dietary influence on protein level in milk and milk yield in dairy cows. Animal Feed Science and Technology 60, 181–190.Google Scholar
Senda, A, Fukuda, K, Ishii, T and Urashima, T 2011. Changes in the bovine whey proteome during the early lactation period. Animal Science Journal 82, 698–706.CrossRefGoogle ScholarPubMed
Shen, JS and Liu, JX 2013. Effects of synchronizing starch and protein degradation on rumen fermentation, nutrient digestibility and production performance in dairy cows. In Proceedings of 3rd International Symposium on Dairy Cow Nutrition and Milk Quality, May 11–13, Beijing, China, pp. 272–273.Google Scholar
Tada, T, Ohkubo, I, Niwa, M, Sasaki, M, Tateyama, H and Eimoto, T 1991. Immunohistochemical localization of Zn-alpha 2-glycoprotein in normal human tissues. The Journal of Histochemistry and Cytochemistry 39, 1221–1226.Google Scholar
Todorov, PT, McDevitt, TM, Meyer, DJ, Ueyama, H, Ohkubo, I and Tisdale, MJ 1998. Purification and characterization of a tumor lipid-mobilizing factor. Cancer Research 58, 2353–2358.Google ScholarPubMed
Treweek, TM 2012. Alpha-casein as a molecular chaperone. In Milk protein (ed. WL Hurley), pp. 85–119. InTech, Rijeka, Croatia.Google Scholar
Yamada, M, Murakami, K, Wallingford, JC and Yuki, Y 2002. Identification of low-abundance proteins of bovine colostral and mature milk using two-dimensional electrophoresis followed by microsequencing and mass spectrometry. Electrophoresis 23, 1153–1160.Google Scholar
Yang, YX, Wang, JQ, Yuan, TJ, Bu, DP, Yang, JH, Sun, P and Zhou, LY 2013. Effects of duodenal infusion of free α-linolenic acid on the plasma and milk proteome of lactating dairy cows. Animal 7, 293–299.Google Scholar