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Gelsolin expression in sheep milk somatic cells during lactation

Published online by Cambridge University Press:  06 March 2019

F. Napolitano Open the ORCID record for F. Napolitano [Opens in a new window] ,
F. Grandoni ,
F. Signorelli ,
G. Annicchiarico Open the ORCID record for G. Annicchiarico [Opens in a new window] ,
G. Catillo Open the ORCID record for G. Catillo [Opens in a new window] ,
B. Moioli Open the ORCID record for B. Moioli [Opens in a new window] ,
A. Crisà Open the ORCID record for A. Crisà [Opens in a new window]  and
C. Marchitelli Open the ORCID record for C. Marchitelli [Opens in a new window]
F. Napolitano*
Affiliation:
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (Council for Agricultural Research and Economics) – CREA-ZA, Centro di ricerca Zootecnia e Acquacoltura (Research Centre for Animal Production and Aquaculture), Via Salaria 31, 00015 Monterotondo, Italy
F. Grandoni
Affiliation:
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (Council for Agricultural Research and Economics) – CREA-ZA, Centro di ricerca Zootecnia e Acquacoltura (Research Centre for Animal Production and Aquaculture), Via Salaria 31, 00015 Monterotondo, Italy
F. Signorelli
Affiliation:
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (Council for Agricultural Research and Economics) – CREA-ZA, Centro di ricerca Zootecnia e Acquacoltura (Research Centre for Animal Production and Aquaculture), Via Salaria 31, 00015 Monterotondo, Italy
G. Annicchiarico
Affiliation:
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (Council for Agricultural Research and Economics) – CREA-ZA, Centro di ricerca Zootecnia e Acquacoltura (Research Centre for Animal Production and Aquaculture), Via Salaria 31, 00015 Monterotondo, Italy
G. Catillo
Affiliation:
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (Council for Agricultural Research and Economics) – CREA-ZA, Centro di ricerca Zootecnia e Acquacoltura (Research Centre for Animal Production and Aquaculture), Via Salaria 31, 00015 Monterotondo, Italy
B. Moioli
Affiliation:
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (Council for Agricultural Research and Economics) – CREA-ZA, Centro di ricerca Zootecnia e Acquacoltura (Research Centre for Animal Production and Aquaculture), Via Salaria 31, 00015 Monterotondo, Italy
A. Crisà
Affiliation:
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (Council for Agricultural Research and Economics) – CREA-ZA, Centro di ricerca Zootecnia e Acquacoltura (Research Centre for Animal Production and Aquaculture), Via Salaria 31, 00015 Monterotondo, Italy
C. Marchitelli
Affiliation:
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (Council for Agricultural Research and Economics) – CREA-ZA, Centro di ricerca Zootecnia e Acquacoltura (Research Centre for Animal Production and Aquaculture), Via Salaria 31, 00015 Monterotondo, Italy
*
†E-mail: francesco.napolitano@crea.gov.it
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Abstract

The identification of genes involved in phenotypes related to milk quality is important for both economic and health aspects in livestock production. The aim of this study was to assess the level of gelsolin gene expression in two breeds of dairy sheep – Sarda and Gentile – with pronounced differences in quantitative and qualitative milk traits. Gelsolin, a type of actin-modulating proteins is involved in the processes of actin remodeling during cell growth and apoptosis; therefore a role of this protein in mammary changes during lactation was here hypothesized. Individual milk samples were collected three times during lactation from 26 ewes of the two breeds. The differential gene expression of gelsolin in the two breeds and the three lactation times was estimated by quantitative PCR on RNA extracted from milk somatic cells. Correlations of gelsolin gene expression with milk yield and quality and days of lactation were also estimated. The results showed that gelsolin gene expression was significantly higher in the Sarda compared to the Gentile at each lactation stage, in agreement with the longer lactation duration and the higher daily milk yield of the first breed. Significant correlations of gelsolin gene expression were found with milk fat content in Sarda breed (−0.46, P<0.05). Gelsolin expression analysis confirmed the link between gelsolin gene function and milk fat content of sheep.

Keywords

dairy sheepgene expressiongelsolinmilk fatmilk somatic cells
Type
Research Article
Information
animal , Volume 13 , Issue 10 , October 2019 , pp. 2297 - 2304
Copyright
© The Animal Consortium 2019 

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