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Lipid-deprived diet perturbs O-glycosylation of secretory proteins in rat mammary epithelial cells

Published online by Cambridge University Press:  01 April 2008

F. Lavialle  and
E. Chanat
F. Lavialle
Affiliation:
INRA, U1196 Génomique et Physiologie de la Lactation, Domaine de Vilvert, F-78352 Jouy-en-Josas Cedex, France
E. Chanat*
Affiliation:
INRA, U1196 Génomique et Physiologie de la Lactation, Domaine de Vilvert, F-78352 Jouy-en-Josas Cedex, France
*
E-mail: eric.chanat@jouy.inra.fr
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Abstract

Nutrition modulates both production and composition of milk. Milk composition was studied in rats chronically fed a diet without additional lipids, and therefore eating only traces of the recommended supply of essential polyunsaturated fatty acid. Despite a large decrease in milk-protein synthesis, only protein composition, but not protein concentration, was found to change in the milk of rats following a lipid-deprived diet. Correlatively, we observed a substantial increase in the lactose concentration of milk. Analysis of milk proteins by two-dimensional electrophoresis demonstrated that the relative proportion of the various molecular forms of κ-casein, an O-glycosylated protein, was modified in the milk of rats receiving the lipid-deprived diet. In tissues, differences in the two-dimensional pattern of κ-casein between control and lipid-deprived rats were similar, if not identical. In contrast to κ-casein, the molecular forms of α-lactalbumin, an N-glycosylated protein, were not affected by the diet. These data provide evidence that O-glycosylation of milk proteins in the secretory pathway of mammary epithelial cells is modulated by the lipid content of experimental diets.

Keywords

epithelial cellslipidsmilk proteinnutritionprotein glycosylation
Type
Full Paper
Information
animal , Volume 2 , Issue 4 , April 2008 , pp. 491 - 499
Copyright
Copyright © The Animal Consortium 2008

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