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Analysis of bacterial community shifts in the gastrointestinal tract of pigs fed diets supplemented with β-glucan from Laminaria digitata, Laminaria hyperborea and Saccharomyces cerevisiae

Published online by Cambridge University Press:  28 February 2013

P. Murphy ,
F. Dal Bello ,
J. O'Doherty ,
E. K. Arendt ,
T. Sweeney  and
A. Coffey
P. Murphy
Affiliation:
Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland
F. Dal Bello
Affiliation:
Department of Food and Nutritional Sciences, University College Cork, Cork, Ireland
J. O'Doherty
Affiliation:
School of Agriculture and Food Science, University College Dublin, Lyons Research farm, Newcastle, Co Dublin, Ireland
E. K. Arendt
Affiliation:
Department of Food and Nutritional Sciences, University College Cork, Cork, Ireland
T. Sweeney
Affiliation:
School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
A. Coffey*
Affiliation:
Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland
*
†E-mail: aidan.coffey@cit.ie
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Abstract

This study was designed to evaluate the effects of algal and yeast β-glucans on the porcine gastrointestinal microbiota, specifically the community of Lactobacillus, Bifidobacterium and coliforms. A total of 48 pigs were fed four diets over a 28-day period to determine the effect that each had on these communities. The control diet consisted of wheat and soya bean meal. The remaining three diets contained wheat and soya bean meal supplemented with β-glucan at 250 g/tonne from Laminaria digitata, Laminaria hyperborea or Saccharomyces cerevisiae. Faecal samples were collected from animals before feeding each diet and after the feeding period. The animals were slaughtered the following day and samples were collected from the stomach, ileum, caecum, proximal colon and distal colon. Alterations in Lactobacillus in the gastrointestinal tract (GIT) were analysed using denaturing gradient gel electrophoresis (DGGE) profiles generated by group-specific 16S rRNA gene PCR amplicons. Plate count analysis was also performed to quantify total coliforms. DGGE profiles indicated that all β-glucan diets provoked the emergence of a richer community of Lactobacillus. The richest community of lactobacilli emerged after feeding L. digitata (LD β-glucan). Plate count analysis revealed that the L. hyperborea (LH β-glucan) diet had a statistically significant effect on the coliform counts in the proximal colon in comparison with the control diet. β-glucan from L. digitata and S. cerevisiae also generally reduced coliforms but to a lesser extent. Nevertheless, the β-glucan diets did not significantly reduce levels of Lactobacillus or Bifidobacterium. DGGE analysis of GIT samples indicated that the three β-glucan diets generally promoted the establishment of a more varied range of Lactobacillus species in the caecum, proximal and distal colon. The LH β-glucan had the most profound reducing effect on coliform counts when compared with the control diet and diets supplemented with L. digitata and S. cerevisiae β-glucans.

Keywords

β-glucanspigsalgaeyeastdenaturing gradient gel electrophoresis
Type
Nutrition
Information
animal , Volume 7 , Issue 7 , July 2013 , pp. 1079 - 1087
Copyright
Copyright © The Animal Consortium 2013 

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