Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-13T06:03:37.526Z Has data issue: false hasContentIssue false
  • English
  • Français

Association analysis between pseudorabies antibody and five single-nucleotide polymorphisms in pigs

Published online by Cambridge University Press:  01 October 2009

S. J. Zhang ,
O. Jafer ,
J. F. Yuan ,
H. C. Chen ,
C. A. Sargent ,
B. Wu ,
R. Zhou ,
L. G. Yang ,
H. Liu  and
J. J. Wu
...Show all authors
S. J. Zhang*
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK
O. Jafer
Affiliation:
Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK
J. F. Yuan
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK
H. C. Chen
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
C. A. Sargent
Affiliation:
Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK
B. Wu
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
R. Zhou
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
L. G. Yang
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
H. Liu
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
J. J. Wu
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
H. B. Liu
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
Q. Tong
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
Y. Yu
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, HuaZhong Agricultural University, 430070, China
N. A. Affara
Affiliation:
Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK
*
†E-mail: sjxiaozhang@hotmail.com
Get access

Abstract

Pseudorabies has become endemic and represents a widespread problem for pig production in the world, causing great economic losses associated with reproductive failure and neonatal mortality in the pig industry. Most diseases are the results of mutations of functional genes. Single-nucleotide polymorphisms (SNPs) from the coding regions of the mediators of pro-inflammatory responses or other candidate genes in pigs could indicate their potential involvement in susceptibility or resistance to PrV (pseudorabies virus) infection. There have been no previous association studies with candidate host genes that may influence PrV phenotypic traits. In order to perform association studies to identify genes contributing to PrV phenotypes, the genotypes of five SNPs from four genes (IL10, CXCL12, BAT2 and EHMT2) were determined for 178 sow samples using a high throughput microarray-based methodology. PrV antibodies were tested by enzyme-linked immunosorbent assay (ELISA) to determine whether there was an association between antibody levels and particular genotypes. The association between SNP genotypes and the PrV antibody levels were analysed using the Duncan method of one-way ANOVA procedure using the SAS (Statistical Analysis Systems) software package. The results showed that the glycoprotein E-ELISA antibody level of pigs with genotypes 11(AA) and 12(AG) was significantly higher than in pigs with genotype 22(GG) (P < 0.05) of SNP in the gene EHMT2-SNP2. The SNP of EHMT2 may be an effective potential tool to identify susceptible and resistant animals when used in conjunction with traditional selection methods.

Keywords

PrVSNPEHMT2ELISAgenotyping of microarray
Type
Full Paper
Information
animal , Volume 3 , Issue 10 , October 2009 , pp. 1363 - 1367
Copyright
Copyright © The Animal Consortium 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ao, JQ, Wang, JW, Chen, XH, Wang, XZ, Long, QX 2003. Expression of pseudorabies virus gE epitopes in Pichia pastoris and its utilization in an indirect PrV gE-ELISA. Journal of Virological Methods 114, 145–150.CrossRefGoogle Scholar
Baner, JI, Sands, J, Storominger, LJ, Spiest, T 1990. A gene pair from the human major histocompatibility complex encodes large proline-rich proteins with multiple repeated motifs and a single ubiquitin-like domain. The Proceedings of the National Academy of Sciences, USA 87, 2374–2378.CrossRefGoogle Scholar
Corn, JL, Stallknecht, DE, Mechlin, NM, Luttrell, MP, Fischer, JR 2004. Persistence of pseudorabies virus in feral swine populations. Journal of Wildlife Diseases 40, 307–310.CrossRefGoogle ScholarPubMed
Fang, S, Miao, J, Xiang, LJ, Ponugoti, B, Treuter, E, Kemper, JK 2007. Coordinated recruitment of histone methyltransferase G9a and other chromatin-modifying enzymes in SHP-mediated regulation of hepatic bile acid metabolism. Molecular and Cellular Biology 27, 1407–1424.CrossRefGoogle ScholarPubMed
Jacobs, L, Mulder, WA, Van Oirschot, JT, Gielkens, AL, Kimman, TG 1993. Deleting two amino acids in glycoprotein gI of pseudorabies virus decreases virulence and neurotropism for pigs, but does not affect immunogenicity. Journal of General Virology 74, 2201–2206.CrossRefGoogle Scholar
Ji, M, Hou, P, Li, S, He, N, Lu, Z 2004. Microarray-based method for genotyping of functional single nucleotide polymorphisms using dual-color fluorescence hybridization. Mutation Research 548, 97–105.CrossRefGoogle ScholarPubMed
Jiwakanon, J, Persson, E, Dalin, AM 2006. The endometrium of the anoestrous female pig: studies on infiltration by cells of the immune system. Reproduction Domestic Animal 41, 191–195.CrossRefGoogle ScholarPubMed
Kamali, SE, Kiany, S, Gharesi, FB, Robati, M 2006. Association study of IL-10 and IFN-gamma gene polymorphisms in Iranian women with preeclampsia. Journal of Reproduction and Immunology 72, 118–126.CrossRefGoogle Scholar
Klipper, AY, Wasserman, M, Braunspiegel, WN, Borstein, D, Peleg, S, Assa, S, Karp, M, Benjamini, Y, Hochberg, Y, Laron, Z 1995. Mathematical formulae for the prediction of the residual beta cell function during the first two years of disease in children and adolescents with insulin-dependent diabetes mellitus. Medical Hypotheses 45, 486–490.CrossRefGoogle Scholar
Lu, M, Grove, EA, Miller, RJ 2002. Abnormal development of the hippocampal dentate gyrus in mice lacking the CXCR4 chemokine receptor. The Proceedings of the National Academy of Sciences, USA 99, 7090–7095.CrossRefGoogle ScholarPubMed
Maha, KK, Noura, BE, Kaouthar, M, Mouna, M, Joumaa, J, Mohamed, A, Ahmed, R, Hammadi, A 2008. A potential role of TNFR gene polymorphisms in autoimmune thyroid diseases in the Tunisian population. Cytokine 43, 110–113.Google Scholar
Nauwynck, HJ 1997. Functional aspects of Aujeszky’s disease (pseudorabies) viral proteins with relation to invasion, virulence and immunogenicity. Veterinary Microbiology 55, 3–11.CrossRefGoogle ScholarPubMed
Peng, H, Kolb, R, Kennedy, JE, Zheng, J 2007. Differential expression of CXCL12 and CXCR4 during human fetal neural progenitor cell differentiation. Neuroimmune Pharmacol 2, 251–258.CrossRefGoogle ScholarPubMed
Reiner, G, Melchinger, E, Kramarova, M 2002. Detection of quantitative trait loci for resistance/susceptibility to pseudorabies virus in swine. Journal of General Virology 83, 167–172.CrossRefGoogle ScholarPubMed
Rothschild, MF, Hill, HT, Christian, LL, Warner, CM 1984. Genetic differences in serum neutralization titers following vaccination with pseudorabies modified live-virus vaccine. American Journal of Veterinary Research 45, 1216.Google Scholar
Schneidersa, A, Thiela, S, Winklera, J, Mollerb, P, Koch, N 2005. Antibodies generated by a novel DNA vaccination identify the MHC class III encoded BAT2 polypeptide. Vaccine 23, 2540–2550.CrossRefGoogle Scholar
Stenson, PD, Ball, EV, Mort, M, Phillips, AD, Shiel, JA, Thomas, NS, Abeysinghe, S, Krawczak, M, Cooper, DN 2003. Human Gene Mutation Database (HGMD): 2003 update. Human Mutation 21, 577–581.CrossRefGoogle ScholarPubMed
Van, OJT, Gielkens, AL, Moormann, RJ, Berns, AJ 1990. Marker vaccines: virus protein-specific antibody assays and the control of Aujeszky’s disease. Veterinary Microbiology 23, 85–101.Google Scholar
Yuan, JF, Moaeen-ud-Din, M, Gong, YZ, Peng, XL, Yang, LG, Feng, YP, Liu, J, Hu, B, Affara, NA, Jafer, O, Zhang, SJ 2007a. Identification of mutations of zona pellucida glycoprotein (ZP3) and its association with pig reproductive traits. Journal of Animal Breeding and Genetics 124, 144–149.CrossRefGoogle ScholarPubMed
Yuan, JF, Jafer, O, Affara, NA, Gong, YZ, Yang, LG, Liu, J, Moaeen-ud-Din, M, Li, WM, Zhang, SJ 2007b. Association of four new single-nucleotide polymorphisms in follicle-stimulating hormone receptor and zona pellucida glycoprotein with reproductive traits in pigs. Animal 1, 1249–1253.CrossRefGoogle ScholarPubMed