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Carbohydrate and amino acid metabolism and oxidative status in Holstein heifers precision-fed diets with different forage to concentrate ratios

Published online by Cambridge University Press:  30 June 2020

J. Zhang Open the ORCID record for J. Zhang [Opens in a new window] ,
H. T. Shi ,
Y. C. Wang ,
S. L. Li ,
Z. J. Cao ,
H. J. Yang  and
Y. J. Wang
J. Zhang
Affiliation:
Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing100193, China
H. T. Shi
Affiliation:
Department of Animal Science, College of Life Science and Technology, Southwest Minzu University, 16# First Ring Road, Chengdu, Sichuan610041, China
Y. C. Wang
Affiliation:
Department of Animal Breeding and Genetics, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing100193, China
S. L. Li
Affiliation:
Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing100193, China
Z. J. Cao
Affiliation:
Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing100193, China
H. J. Yang
Affiliation:
Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing100193, China
Y. J. Wang*
Affiliation:
Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing100193, China
*
†E-mail: yajingwang@cau.edu.cn
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Abstract

Previous work led to the proposal that the precision feeding of a high-concentrate diet may represent a potential method with which to enhance feed efficiency (FE) when rearing dairy heifers. However, the physiological and metabolic mechanisms underlying this approach remain unclear. This study used metabolomics analysis to investigate the changes in plasma metabolites of heifers precision-fed diets containing a wide range of forage to concentrate ratios. Twenty-four half-sib Holstein heifers, with a similar body condition, were randomly assigned into four groups and precision fed with diets containing different proportions of concentrate (20%, 40%, 60% and 80% based on DM). After 28 days of feeding, blood samples were collected 6 h after morning feeding and gas chromatography time-of-flight/MS was used to analyze the plasma samples. Parameters of oxidative status were also determined in the plasma. The FE (after being corrected for gut fill) increased linearly (P < 0.01) with increasing level of dietary concentrate. Significant changes were identified for 38 different metabolites in the plasma of heifers fed different dietary forage to concentrate ratios. The main pathways showing alterations were clustered into those relating to carbohydrate and amino acid metabolism; all of which have been previously associated with FE changes in ruminants. Heifers fed with a high-concentrate diet had higher (P < 0.01) plasma total antioxidant capacity and superoxide dismutase but lower (P ≤ 0.02) hydroxyl radical and hydrogen peroxide than heifers fed with a low-concentrate diet, which might indicate a lower plasma oxidative status in the heifers fed a high-concentrate diet. Thus, heifers fed with a high-concentrate diet had higher FE and antioxidant capacity but a lower plasma oxidative status as well as changed carbohydrate and amino acid metabolism. Our findings provide a better understanding of how forage to concentrate ratios affect FE and metabolism in the precision-fed growing heifers.

Keywords

metabolomicshigh concentratefeed efficiencyredoxheifer
Type
Research Article
Information
animal , Volume 14 , Issue 11 , November 2020 , pp. 2315 - 2325
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of The Animal Consortium

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