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Concentration of the mammalian lignans enterolactone and enterodiol in milk of cows fed diets containing different concentrations of whole flaxseed*

Published online by Cambridge University Press:  01 October 2009

H. V. Petit  and
N. Gagnon
H. V. Petit*
Affiliation:
Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, PO Box 90, Station Lennoxville, Sherbrooke, QC J1M 1Z3, Canada
N. Gagnon
Affiliation:
Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, PO Box 90, Station Lennoxville, Sherbrooke, QC J1M 1Z3, Canada
*
†E-mail: petith@agr.gc.ca
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Abstract

A total of 32 lactating Holstein cows with mean body weight of 622 kg (s.e. = 24) were allotted, at week 25 of lactation, to eight groups of four cows blocked for similar days in milk. The objective of the experiment was to determine the effect of feeding four dietary concentrations (0, 50, 100 or 150 g/kg of dry matter) of whole flaxseed, which contains the plant lignan precursor secoisolariciresinol diglucoside (SDG), on concentrations of two mammalian lignans (enterodiol and enterolactone) in milk. The effects of the four diets on feed intake, milk production, milk composition and digestion were also studied. Cows within each block were assigned to one of the four isonitrogenous and isoenergetic total mixed diets and the experiment was carried out from week 25 to 29 of lactation. Diets were fed for ad libitum intake. Enterolactone was the mammalian lignan, of the two metabolites studied, detected in the milk of cows and its concentration in milk tended (P = 0.08) to increase linearly with higher intake of SDG in the diet. Feed intake, milk yield and milk composition were similar among diets. Milk fatty acid profile was slightly improved by feeding flaxseed, as shown by higher concentrations of fatty acids (e.g. n-3) recognized as being beneficial for human health. Those results suggest that feeding of whole flaxseed may result in changes in milk fatty acid composition and enterolactone content, which offer benefits for consumers.

Keywords

dairyflaxseedantioxidantsmilk productionfatty acids
Type
Full Paper
Information
animal , Volume 3 , Issue 10 , October 2009 , pp. 1428 - 1435
Copyright
Copyright © The Animal Consortium 2009

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Footnotes

*

Contribution Number 1007 from the Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, PO Box 90, Lennoxville QC, Canada J1M 1Z3.

References

Ambrose, D, Kastelic, JP, Corbett, R, Pitney, PA, Petit, HV, Small, JA, Zalkovic, P 2006. Lower pregnancy losses in lactating dairy cows fed a diet enriched in alpha-linolenic acid. Journal of Dairy Science 89, 3066–3074.CrossRefGoogle ScholarPubMed
Andersen, C, Weisbjerg, MR, Hansen-Møller, J, Sejrsen, K 2009. Effect of forage on the content of phyto-oestrogens in bovine milk. Animal 3, 617–622.CrossRefGoogle ScholarPubMed
Antignac, JP, Cariou, R, Le Bizec, B, André, F 2004. New data regarding phytoestrogens content in bovine milk. Food Chemistry 87, 275–281.CrossRefGoogle Scholar
Ashes, JR, St Vincent Welch, P, Gulati, SK, Scott, TW, Brown, GH, Blakelyel, S 1992. Manipulation of the fatty acid composition of milk by feeding protected canola seeds. Journal of Dairy Science 75, 1090–1096.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists 1990. Official methods of analysis, 15th edition. AOAC, Washington, DC, USA.Google Scholar
Axelson, M, Sjovall, J, Gustafsson, BE, Setchell, KDR 1982. Origin of lignans in mammals and identification of a precursor from plants. Nature 298, 659–660.CrossRefGoogle ScholarPubMed
Canadian Council on Animal Care 1993. Guide to the care and use of experimental animals, (ed. ED Olfert, BM Cross and AA McWilliam), vol. 1. CCAC, Ottawa, ON, Canada.Google Scholar
Côrtes, C, Gagnon, N, Benchaar, C, da Silva, D, Santos, GTD, Petit, HV 2008. In vitro metabolism of flax lignans by ruminal and fecal microbiota of dairy cows. Journal of Applied Microbiology 105, 1585–1594.CrossRefGoogle ScholarPubMed
Cunnane, SC, Hamadeh, MJ, Liede, AC, Thompson, LU, Wolever, TM, Jenkins, DJ 1995. Nutritional attributes of traditional flaxseed in healthy young adults. The American Journal of Clinical Nutrition 61, 62–68.CrossRefGoogle ScholarPubMed
Dehennin, L, Reiffsteck, A, Jondet, M, Thibier, M 1982. Identification and quantitative estimation of a lignan in human and bovine semen. Journal of Reproduction and Fertility 66, 305–309.CrossRefGoogle ScholarPubMed
Dhiman, TR, Satter, LD, Pariza, MW, Galli, MP, Albridht, K, Tolosa, MX 2000. Conjugated linoleic acid (CLA) content of milk from cows offered diets rich in linoleic and linolenic acid. Journal of Dairy Science 83, 1016–1027.CrossRefGoogle Scholar
Doreau, M, Aurousseau, E, Martin, C 2009a. Effects of linseed lipids fed as rolled seeds, extruded seeds or oil on organic matter and crude protein digestion in cows. Animal Feed Science and Technology 150, 187–196.CrossRefGoogle Scholar
Doreau, M, Laverroux, S, Normand, J, Chesneau, G, Glasser, F 2009b. Effects of linseed fed as rolled seeds, extruded seeds or oil on fatty acid rumen metabolism and intestinal digestibility in cows. Lipids 44, 53–62.CrossRefGoogle ScholarPubMed
Eliasson, C, Kamal-Eldin, A, Andersson, R, Åman, P 2003. High-performance liquid chromatographic analysis of secoisolariciresinol diglucoside and hydroxycinnamic acid glucosides in flaxseed by alkaline extraction. Journal of Chromatography A 1012, 151–159.CrossRefGoogle ScholarPubMed
Frank, AA, Custer, LJ 1996. Diadzein and genistein concentrations in human milk after soy consumption. Clinical Chemistry 42, 955–964.CrossRefGoogle Scholar
Gagnon, N, Côrtes, C, da Silva, D, Kazama, R, dos Santos, G, Zeoula, L, Benchaar, C, Petit, HV 2009. Ruminal metabolism of flaxseed (Linum usitatissimum) lignans to the mammalian lignan enterolactone and its concentration in ruminal fluid, plasma, urine, and milk of dairy cows. The British Journal of Nutrition, doi: 10.1017/S0007114509344104.CrossRefGoogle Scholar
Glasser, F, Ferlay, A, Chilliard, Y 2008. Oilseed lipid supplements and fatty acid composition of cow milk: a meta-analysis. Journal of Dairy Science 91, 4687–4703.CrossRefGoogle ScholarPubMed
Gonthier, C, Mustafa, AF, Berthiaume, R, Petit, HV, Martineau, R, Ouellet, DR 2004. Effects of feeding micronized and extruded flaxseed on ruminal fermentation and nutrient utilization by dairy cows. Journal of Dairy Science 87, 1854–1863.CrossRefGoogle ScholarPubMed
Hansen-Møller, J, Kristensen, NB 2004. Determination of phytoestrogens in bovine plasma using liquid chromatography and tandem mass spectrometry. Journal of Animal Feed Science 13 (suppl. 1), 315–318.CrossRefGoogle Scholar
Johnsson, P, Kamal-Eldin, A, Lundgren, LN, Aman, P 2000. HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds. Journal of Agriculture and Food Chemistry 48, 5216–5219.CrossRefGoogle ScholarPubMed
Kennelly, JJ 1996. The fatty acid composition of milk as influenced by feeding oilseeds. Animal Feed Science and Technology 60, 137–152.CrossRefGoogle Scholar
Kilkkinen, A, Stumpf, K, Pietinen, P, Valsta, LM, Tapanainen, H, Adlercreutz, H 2001. Determinants of serum enterolactone concentration. The American Journal of Clinical Nutrition 73, 1094–1100.CrossRefGoogle ScholarPubMed
Kuijsten, A, Arts, ICW, van’t Veer, P, Hollman, CH 2005. The relative bioavailability of enterolignans in humans is enhanced by milling and crushing of flaxseed. The Journal of Nutrition 135, 2812–2816.CrossRefGoogle ScholarPubMed
Martin, C, Rouel, J, Jouany, JP, Doreau, M, Chilliard, Y 2008. Methane output and diet digestibility in response to feeding dairy cows crude linseed, extruded linseed, or linseed oil. Journal of Animal Science 86, 2642–2650.CrossRefGoogle ScholarPubMed
Muir, AD, Westcott, ND 2000. Quantification of the lignan secoisolariciresinol diglucoside in baked goods containing flax seed or flax meal. Journal of Agricultural and Food Chemistry 48, 4048–4052.CrossRefGoogle ScholarPubMed
Murkies, AL, Wilcox, G, Davis, SR 1998. Phytoestrogens. The Journal of Clinical Endocrinology and Metabolism 83, 297–303.Google ScholarPubMed
National Research Council 2001. Nutrient requirements of dairy cattle, 7th revised edition. National Academy Press, Washington, DC, USA.Google Scholar
Peñalvo, JL, Haajanen, KM, Botting, N, Adlercreutz, H 2005. Quantification of lignans in food using isotope dilution gas chromatography/mass spectrometry. Journal of Agricultural and Food Chemistry 53, 9342–9347.CrossRefGoogle ScholarPubMed
Petit, HV 2002. Digestion, milk production, milk composition, and blood composition of dairy cows fed whole flaxseed. Journal of Dairy Science 85, 1482–1490.CrossRefGoogle ScholarPubMed
Petit, HV, Benchaar, C 2007. Milk production, milk composition, blood composition, and conception rate of transition dairy cows fed different fat sources. Canadian Journal of Animal Science 87, 591–600.CrossRefGoogle Scholar
Petit, HV, Gagnon, N 2009. Milk concentrations of the mammalian lignans enterolactone and enterodiol, milk production, and whole tract digestibility of dairy cows fed diets containing different concentrations of flaxseed meal. Animal Feed Science and Technology 152, 103–111.CrossRefGoogle Scholar
Petit, HV, Germiquet, C, LeBel, D 2004. Effect of feeding whole unprocessed sunflower seeds and flaxseed on milk production, milk composition, and prostaglandin secretion in dairy cows. Journal of Dairy Science 87, 3889–3898.CrossRefGoogle ScholarPubMed
Petit, HV, Twagiramungu, H 2006. Conception rate and reproductive function of dairy cows fed different fat sources. Theriogenology 66, 1316–1324.CrossRefGoogle ScholarPubMed
Prasad, K 2000. Antioxidant activity of secoisolariciresinol diglycoside-derived metabolites, secoisolariciresinol, enterodiol, and enterolactone. The International Journal of Angiology 9, 220–225.CrossRefGoogle ScholarPubMed
Rowan, AM, Haggarty, NW, Ram, S 2005. Milk bioactives: discovery and proof of concept Australian. Journal of Dairy Technology 60, 114–118.Google Scholar
Saarinen, NM, Smeds, A, Mäkelä, SI, Ämmälä, J, Hakala, K, Pihlava, JM, Ryhänen, EL, Sjöholm, R, Santti, R 2002. Structural determinants of plant lignans for the formation of enterolactone in vivo. Journal of Chromatography B 777, 311–319.CrossRefGoogle ScholarPubMed
Secchiari, P, Antongiovanni, M, Mele, M, Serra, A, Buccioni, A, Ferruzzi, G, Paoletti, F, Petacchi, F 2003. Effect of kind of dietary fat on the quality of milk fat from Italian Friesian cows. Livestock and Production Science 83, 43–52.CrossRefGoogle Scholar
Setchell, KDR, Lawson, AM, Mitchell, FL, Adlercreutz, H, Kirk, DN, Axelson, M 1980. Lignans in man and in animal species. Nature 287, 740–742.CrossRefGoogle ScholarPubMed
Smeds, AI, Eklund, PC, Sjoholm, RE, Willfor, SM, Nishibe, S, Deyama, T, Holmbom, BR 2007. Quantification of a broad spectrum of lignans in cereals, oilseeds, and nuts. Journal of Agricultural Food Chemistry 55, 1337–1346.CrossRefGoogle ScholarPubMed
Steinshamn, H, Purup, S, Thuen, E, Hansen-Møller, J 2008. Effects of clover-grass silages and concentrate supplementation on the content of phytoestrogens in dairy cow milk. Journal of Dairy Science 91, 2715–2725.CrossRefGoogle ScholarPubMed
Tou, JCL, Chen, J, Thompson, LU 1998. Flaxseed and its lignan precursor, secoisolariciresinol diglycoside, affect pregnancy outcome and reproductive development in rats. The Journal of Nutrition 128, 1861–1868.CrossRefGoogle ScholarPubMed
Van Soest, PJ, Robertson, JB, Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.CrossRefGoogle ScholarPubMed
Wright, T, McBride, B, Holub, B 1998. Docosahexaenoic acid-enriched milk. World Review of Nutrition and Dietetics 83, 160–165.CrossRefGoogle ScholarPubMed
Zhou, W, Wang, G, Han, Z, Yao, W, Zhu, W 2009. Metabolism of flaxseed lignans in the rumen and its impact on ruminal metabolism and flora. Animal Feed Science and Technology 150, 18–26.CrossRefGoogle Scholar