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A diet containing native or fermented wheat bran does not interfere with natural microbiota of laying hens

Published online by Cambridge University Press:  15 January 2020

E. Wanzenböck ,
U. Zitz ,
C. Steinbauer ,
W. Kneifel ,
K. J. Domig  and
K. Schedle Open the ORCID record for K. Schedle [Opens in a new window]
E. Wanzenböck
Affiliation:
Institute of Food Science, University of Natural Resources and Life Sciences Vienna, Muthgasse18, 1190Vienna, Austria
U. Zitz
Affiliation:
Institute of Food Science, University of Natural Resources and Life Sciences Vienna, Muthgasse18, 1190Vienna, Austria
C. Steinbauer
Affiliation:
Institute of Animal Nutrition, Livestock Products, and Nutrition Physiology, University of Natural Resources and Life Sciences Vienna, Muthgasse 11, 1190Vienna, Austria
W. Kneifel
Affiliation:
Institute of Food Science, University of Natural Resources and Life Sciences Vienna, Muthgasse18, 1190Vienna, Austria
K. J. Domig
Affiliation:
Institute of Food Science, University of Natural Resources and Life Sciences Vienna, Muthgasse18, 1190Vienna, Austria
K. Schedle*
Affiliation:
Institute of Animal Nutrition, Livestock Products, and Nutrition Physiology, University of Natural Resources and Life Sciences Vienna, Muthgasse 11, 1190Vienna, Austria
*
†E-mail: karl.schedle@boku.ac.at
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Abstract

Wheat bran (WB) is an important side product of the milling industry and can serve as dietary fiber compound for monogastric animals. The aim of this study was to evaluate the influence of native or fermented WB on the gut physiology and microbiology of laying hens. To accomplish this, 24 laying hens were fed the following diets: conventional diet without WB; 15% native WB in the diet; 15% WB fermented with Pleurotus eryngii; and 15% WB fermented with P. eryngii and a lactic acid bacterial culture. Immediately after slaughtering, digesta samples were taken from the jejunum, ileum and cecum, respectively. Total DNA was extracted and subsequently investigated with 16S DNA amplicon sequencing. Neither native nor fermented WB supplementations negatively affected the feed conversion ratio, laying performance or the relative abundances and alpha-diversity of microbiota in the intestine. Effects of WB-based diets on gut morphology were only recognized in the jejunum (reduced villum height and mucosa thickness). Likewise, WB supplementation decreased the digestibility of DM and starch. Based on these findings, it was demonstrated that different WB variants are applicable without exerting practically negative consequences on performance or on gut microbiota. Fermentation improved the digestibility/retention of dietary fat and phosphorus. However, no further beneficial effects were observed. This study also allowed a more in-depth view on the laying hens’ gut microbiome and its variation within the gut segments.

Keywords

poultrygut microbiomedigestibility16S rRNAdietary fiber
Type
Research Article
Information
animal , Volume 14 , Issue 6 , June 2020 , pp. 1147 - 1155
Copyright
© The Animal Consortium 2020

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