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Different rates of glycolysis affect glycolytic activities and protein properties in turkey breast muscle

Published online by Cambridge University Press:  15 October 2008

S. Eadmusik ,
C. Molette ,
H. Rémignon  and
X. Fernandez
S. Eadmusik
Affiliation:
INRA, UMR 1289 TANDEM, F-31326 Castanet-Tolosan, France Université de Toulouse, INPT-ENSAT, UMR 1289 TANDEM, F-31326 Castanet-Tolosan, France ENVT, UMR 1289 TANDEM, F-31076 Toulouse, France
C. Molette*
Affiliation:
INRA, UMR 1289 TANDEM, F-31326 Castanet-Tolosan, France Université de Toulouse, INPT-ENSAT, UMR 1289 TANDEM, F-31326 Castanet-Tolosan, France ENVT, UMR 1289 TANDEM, F-31076 Toulouse, France
H. Rémignon
Affiliation:
INRA, UMR 1289 TANDEM, F-31326 Castanet-Tolosan, France Université de Toulouse, INPT-ENSAT, UMR 1289 TANDEM, F-31326 Castanet-Tolosan, France ENVT, UMR 1289 TANDEM, F-31076 Toulouse, France
X. Fernandez
Affiliation:
INRA, UMR 1289 TANDEM, F-31326 Castanet-Tolosan, France Université de Toulouse, INPT-ENSAT, UMR 1289 TANDEM, F-31326 Castanet-Tolosan, France ENVT, UMR 1289 TANDEM, F-31076 Toulouse, France
*
E-mail: molette@ensat.fr
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Abstract

Protein alterations of turkey breast muscles (Pectoralis major) were investigated at 20 min and 24 h post mortem. Specific activities, quantities and kinetic parameters of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and aldolase A were also determined at 20 min post mortem. Based on the pH values at 20 min post mortem, two groups of samples were classified as rapid glycolysis group (RG; pH20 min = 5.80 ± 0.07, n = 20) and normal glycolysis group (NG; pH20 min = 6.21 ± 0.01, n = 20). RG had lower specific activities of GAPDH and aldolase A than NG while Vm and Km values of both enzymes were not different between groups. RG showed lower high ionic strength (HIS) and pellet protein extractabilities at 20 min post mortem. It also had lower low ionic strength (LIS) and HIS protein extractabilities at 24 h post mortem. Besides pellet protein, muscular protein extractabilities at 24 h post mortem were higher than at 20 min post mortem. From SDS-PAGE of samples at 24 h post mortem, RG exhibited lower band intensities at 45 and 200 kDa, which were further identified as actin and myosin heavy chain (MHC), respectively. Western blots revealed that relative amounts of actin and MHC at 20 min post mortem were not different between groups. However, RG muscles had less relative amount of actin at 24 h post mortem. It also indicated that amounts of actin and MHC increased with regard to post mortem time.

Keywords

turkey breast musclealdolase AGAPDHactinmyosin heavy chain
Type
Full Paper
Information
animal , Volume 3 , Issue 2 , February 2009 , pp. 237 - 243
Copyright
Copyright © The Animal Consortium 2008

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