Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-10T14:18:48.197Z Has data issue: false hasContentIssue false
  • English
  • Français

Dose-response effects of woody and herbaceous forage plants on in vitro ruminal methane and ammonia formation, and their short-term palatability in lactating cows

Published online by Cambridge University Press:  02 October 2019

M. Terranova ,
S. Wang ,
L. Eggerschwiler ,
U. Braun ,
M. Kreuzer  and
A. Schwarm
M. Terranova*
Affiliation:
ETH Zurich, Department of Environmental Science, Institute of Agricultural Sciences, Universitätstrasse 2, Zurich 8092, Switzerland
S. Wang
Affiliation:
ETH Zurich, Department of Environmental Science, Institute of Agricultural Sciences, Universitätstrasse 2, Zurich 8092, Switzerland
L. Eggerschwiler
Affiliation:
Agroscope, Tioleyre 4, Posieux 1725, Switzerland
U. Braun
Affiliation:
Vetsuisse Faculty, University of Zurich, Clinic for Ruminants, Zurich 8057, Switzerland
M. Kreuzer
Affiliation:
ETH Zurich, Department of Environmental Science, Institute of Agricultural Sciences, Universitätstrasse 2, Zurich 8092, Switzerland
A. Schwarm
Affiliation:
ETH Zurich, Department of Environmental Science, Institute of Agricultural Sciences, Universitätstrasse 2, Zurich 8092, Switzerland Norwegian University of Life Sciences, Department of Animal and Aquacultural Sciences, Arboretveien 6, 1433 Ås, Norway
*
†E-mail: melissa.terranova@usys.ethz.ch
Get access

Abstract

Plant secondary compounds (PSC) are prevalent in many woody, temperate-climate plant species and play a crucial role in dietary attempts to mitigate methane emissions in ruminants. However, their application requires sufficient palatability and feeding value. In the present study, leaves from silver birch (Betula pendula), hazel (Corylus avellana), blackcurrant (Ribes nigrum), green grape vine (Vitis vinifera) and the herbs rosebay willow (Epilobium angustifolium) and wood avens (Geum urbanum) were tested in various doses with the Hohenheim gas test method in vitro and their short-term palatability in dairy cows. For the palatability experiment, the plants were pelleted with lucerne in different proportions to obtain the same phenol content, but realised contents differed from expected contents. The pellets were provided separately from a mixed basal ration (0.4 : 0.6) to each cow, in a randomised order, for 3 days per plant. All plants mitigated in vitro methane and ammonia formation, often in a linear dose response. These levels of effects differed among plants. Significant effects were observed at 100 (hazel, rosebay willow) to 400 g/kg of plant material. The test plants had a lower feeding value than the high-quality basal diet. This was indicated by in vitro organic matter digestibility, short-chain fatty acid formation and calculated contents of net energy of lactation. Simultaneously, the linear depression of ammonia formation indicated a dose-dependent increase of utilisable CP. Only blackcurrant and birch were less preferred to lucerne. However, this aversion subsided on day 3 of offer. The rosebay willow pellets had the highest phenol content but were not the least palatable. Accordingly, PSC may not be the main determinants of palatability for the plants tested. Plants did not differ significantly in their short-term effects on milk yield and composition, and all of the plants substantially reduced milk urea content. Overall, the results suggest that hazel and vine leaves, and rosebay willow and wood avens herbs should be tested for their potential to mitigate methane and N emissions in vivo.

Keywords

phenolshazelvinerosebay willowwood avens
Type
Research Article
Information
animal , Volume 14 , Issue 3 , March 2020 , pp. 538 - 548
Copyright
© The Animal Consortium 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

a

Present address: APC Microbiome Institute, University College Cork, Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland

References

Aerts, RJ, Barry, TN and McNabb, WC 1999. Polyphenols and agriculture: beneficial effects of proanthocyanidins in forages. Agriculture, Ecosystems & Environment 75, 1–12.CrossRefGoogle Scholar
AOAC International 1997. Official Methods of Analysis. 16th edition. Association of Official Analytical Chemists, Arlington, VA, USA.Google Scholar
Barry, T and McNabb, W 1999. The implications of condensed tannins on the nutritive value of temperate forages fed to ruminants. British Journal of Nutrition 81, 263–272.CrossRefGoogle ScholarPubMed
Beauchemin, KA, Kreuzer, M, O’Mara, F and McAllister, TA 2008. Nutritional management for enteric methane abatement: a review. Australian Journal of Experimental Agriculture 48, 21–27.CrossRefGoogle Scholar
Donnelly, E and Anthony, W 1969. Relationship of tannin, dry matter digestibility and crude protein in Sericea lespedeza 1. Crop Science 9, 361–362.CrossRefGoogle Scholar
Edmunds, B, Südekum, K-H, Spiekers, H, Schuster, M and Schwarz, FJ 2012. Estimating utilisable crude protein at the duodenum, a precursor to metabolisable protein for ruminants, from forages using a modified gas test. Animal Feed Science and Technology 175, 106–113.CrossRefGoogle Scholar
Frutos, P, Hervas, G, Giráldez, F and Mantecón, A 2004. Tannins and ruminant nutrition. Spanish Journal of Agricultural Research 2, 191–202.CrossRefGoogle Scholar
Jayanegara, A, Leiber, F and Kreuzer, M 2012. Meta-analysis of the relationship between dietary tannin level and methane formation in ruminants from in vivo and in vitro experiments. Journal of Animal Physiology and Animal Nutrition 96, 365–375.CrossRefGoogle ScholarPubMed
Jayanegara, A, Wina, E, Soliva, CR, Marquardt, S, Kreuzer, M and Leiber, F 2011. Dependence of forage quality and methanogenic potential of tropical plants on their phenolic fractions as determined by principal component analysis. Animal Feed Science and Technology 163, 231–243.CrossRefGoogle Scholar
Kumar, R and Singh, M 1984. Tannins: their adverse role in ruminant nutrition. Journal of Agricultural and Food Chemistry 32, 447–453.CrossRefGoogle Scholar
Makkar, HPS 2003. Quantification of tannins in tree and shrub foliage: a laboratory manual. Kluwer Academic Publishers, Dordrecht, the Netherlands.CrossRefGoogle Scholar
Meier, J, Liesegang, A, Louhaichi, M, Hilali, M, Rischkowsky, B, Kreuzer, M and Marquardt, S 2014. Intake pattern and nutrient supply of lactating sheep selecting dried forage from woody plants and straw offered in binary or multiple choice. Animal Feed Science and Technology 188, 1–12.CrossRefGoogle Scholar
Menke, KH and Steingass, H 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28, 7–55.Google Scholar
O’Connell, JE and Fox, PF 2001. Significance and applications of phenolic compounds in the production and quality of milk and dairy products: a review. International Dairy Journal 11, 103–120.CrossRefGoogle Scholar
Palo, RT, Sunnerheim, K and Theander, O 1985. Seasonal variation of phenols, crude protein and cell wall content of birch (Betula pendula Roth.) in relation to ruminant in vitro digestibility. Oecologia 65, 314–318.CrossRefGoogle Scholar
Salem, HB, Nefzaoui, A and Abdouli, H 1994. Palatability of shrubs and fodder trees measured on sheep and dromedaries: 1. Methodological approach. Animal Feed Science and Technology 46, 143–153.CrossRefGoogle Scholar
Soliva, C and Hess, HD 2007. Measuring methane emission of ruminants by in vitro and in vivo techniques. In Measuring methane production from ruminants (ed. Makkar, HPS and Vercoe, PE), pp. 15–31. Springer, Dordrecht, The Netherlands.CrossRefGoogle Scholar
Terranova, M, Kreuzer, M, Braun, U and Schwarm, A 2018a. In vitro screening of temperate climate forages from a variety of woody plants for their potential to mitigate ruminal methane and ammonia formation. Journal of Agricultural Science 156, 929–941.CrossRefGoogle Scholar
Terranova, M, Wang, S, Kreuzer, M, Eggerschwiler, L and Schwarm, A 2018b. Palatability of plants rich in phenols and their effect on milk yield and composition in cows. Advances in Animal Biosciences 9 (suppl. 3), 490.Google Scholar
Tiemann, TT, Franco, LH, Peters, M, Frossard, E, Kreuzer, M, Lascano, CE and Hess, HD 2009. Effect of season, soil type and fertilizer on the biomass production and chemical composition of five tropical shrub legumes with forage potential. Grass and Forage Science 64, 255–265.CrossRefGoogle Scholar
Waghorn, GC 2008. Beneficial and detrimental effects of dietary condensed tannins for sustainable sheep and goat production – progress and challenges. Animal Feed Science and Technology 147, 116–139.CrossRefGoogle Scholar
Waghorn, GC, Shelton, ID, McNabb, WC and McCutcheon, SN 1994. Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep. 2. Nitrogenous aspects. The Journal of Agricultural Science 123, 109–119.CrossRefGoogle Scholar
Wang, S, Terranova, M, Kreuzer, M, Marquardt, S, Eggerschwiler, L and Schwarm, A 2018. Supplementation of pelleted hazel (Corylus avellana) leaves decreases methane and urinary nitrogen emissions by sheep at unchanged forage intake. Scientific Reports 8, 5427, https://doi.org//10.1038/s41598-018-23572-3.CrossRefGoogle ScholarPubMed
Supplementary material: File

Terranova et al. supplementary material

Tables S1-S5

Download Terranova et al. supplementary material(File)
File 75.8 KB