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Effect of breed on the expression of Sirtuins (Sirt1-7) and antioxidant capacity in porcine brain

Published online by Cambridge University Press:  08 October 2013

Y. Ren ,
T. Z. Shan ,
L. N. Zhu ,
T. Wu ,
J. Guo  and
Y. Z. Wang
Y. Ren
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
T. Z. Shan
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
L. N. Zhu
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
T. Wu
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
J. Guo
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
Y. Z. Wang*
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
*
†E-mail: yzwang321@zju.edu.cn
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Abstract

Sirtuins, NAD-dependent histone deacetylase (HDAC), are correlated to aging and antioxidant. The aim of this study was to determine breed differences of porcine Sirtuins expression and antioxidant capacity in brain between Jinhua pigs (a fatty breed of China) and Danish Landrace pigs (a leaner breed). Effect of age on Sirtuins’ expression was also investigated. At the age of 180 days, the mRNA levels of Sirt1, as well as Sirt2 and Sirt4, in Jinhua pigs were greater, but the mRNA levels of Sirt3, Sirt5, Sirt6, and Sirt7 of Jinhua pigs were lower compared with Danish Landrace pigs. Likewise, at the same BW of 64 kg, the mRNA levels of Sirtuins, except Sirt5 and Sirt7, in Jinhua pigs were greater than Danish Landrace pigs. Meanwhile, Jinhua pigs possessed higher antioxidants activity than Danish Landrace pigs either at the same age or at the same BW. Furthermore, mRNA levels of Sirtuins were decreased with age in brain of the two breeds from 30 to 120 days. The results indicated that Sirtuins expression in brain was different between fatty and lean pigs, and Sirtuins expression may be correlated to antioxidant capacity. In addition, age could down-regulate Siruins expression in porcine brain.

Keywords

ageantioxidantbrainpigsSirtuins
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 12 , December 2013 , pp. 1994 - 1998
Copyright
Copyright © The Animal Consortium 2013 

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Footnotes

a

These authors contributed equally to this work.

References

Albani, D, Polito, L and Forloni, G 2010. Sirtuins as novel targets for Alzheimer's disease and other neurodegenerative disorders: experimental and genetic evidence. Journal of Alzheimers Disease 19, 11–26.Google Scholar
Alcendor, RR, Gao, S, Zhai, P, Zablocki, D, Holle, E, Yu, X, Tian, B, Wagner, T, Vatner, SF and Sadoshima, J 2007. Sirt1 regulates aging and resistance to oxidative stress in the heart. Circulation Research 100, 1512–1521.Google Scholar
Bause, AS and Haigis, MC 2013. Sirt3 regulation of mitochondrial oxidative stress. Experimental Gerontology 48, 634–639.Google Scholar
Bell, EL and Guarente, L 2011. The Sirt3 divining rod points to oxidative stress. Molecular Cell 42, 561–568.Google Scholar
Beltowski, J, Wojcicka, G, Gorny, D and Marciniak, A 2000. The effect of dietary-induced obesity on lipid peroxidation, antioxidant enzymes and total plasma antioxidant capacity. Journal of Physiology and Pharmacology 51, 883–896.Google Scholar
Chong, ZZ, Shang, YC, Wang, S and Maiese, K 2012. Sirt1: new avenues of discovery for disorders of oxidative stress. Expert Opinion on Therapeutic Targets 16, 167–178.Google Scholar
Chrysohoou, C, Panagiotakos, DB, Pitsavos, C, Skoumas, I, Papademetriou, L, Economou, M and Stefanadis, C 2007. The implication of obesity on total antioxidant capacity in apparently healthy men and women: the ATTICA study. Nutrition, Metabolism, and Cardiovascular Diseases 17, 590–597.Google Scholar
Cross, WL, Roby, MA, Deschenes, MR and Harris, MB 2008. Myocardial SIRT1 expression following endurance and resistance exercise training in young and old rats. FASEB Journal 22, 753.CrossRefGoogle Scholar
Donmez, G and Guarente, L 2010. Aging and disease: connections to sirtuins. Aging Cell 9, 285–290.Google Scholar
Engel, N and Mahlknecht, U 2008. Aging and anti-aging: unexpected side effects of everyday medication through sirtuin1 modulation. International Journal of Molecular Medicine 21, 223–232.Google Scholar
Ferrara, N, Rinaldi, B, Corbi, G, Conti, V, Stiuso, P, Boccuti, S, Rengo, G, Rossi, F and Filippelli, A 2008. Exercise training promotes Sirt1 activity in aged rats. Rejuvenation Research 11, 139–150.Google Scholar
Gan, L and Mucke, L 2008. Paths of convergence: sirtuins in aging and neurodegeneration. Neuron 58, 10–14.Google Scholar
Ghinis-Hozumi, Y, Antaramian, A, Villarroya, F, Pina, E and Mora, O 2013. Potential role of sirtuins in livestock production. Animal 7, 101–108.Google Scholar
Guo, J, Shan, T, Wu, T, Zhu, LN, Ren, Y, An, S and Wang, Y 2011. Comparisons of different muscle metabolic enzymes and muscle fiber types in Jinhua and Landrace pigs. Journal of Animal Science 89, 185–191.Google Scholar
Haigis, MC and Guarente, LP 2006. Mammalian sirtuins – emerging roles in physiology, aging, and calorie restriction. Genes & Development 20, 2913–2921.CrossRefGoogle ScholarPubMed
Haigis, MC and Sinclair, DA 2010. Mammalian sirtuins: biological insights and disease relevance. Annual Review of Pathology 5, 253–295.Google Scholar
Hernandez, P, Zomeno, L, Arino, B and Blasco, A 2004. Antioxidant, lipolytic and proteolytic enzyme activities in pork meat from different genotypes. Meat Science 66, 525–529.CrossRefGoogle ScholarPubMed
Jin, D, Tan, HJ, Lei, T, Gan, L, Chen, XD, Long, QQ, Feng, B and Yang, ZQ 2009. Molecular cloning and characterization of porcine sirtuin genes. Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology 153, 348–358.Google Scholar
Kubota, S, Kurihara, T, Mochimaru, H, Satofuka, S, Noda, K, Ozawa, Y, Oike, Y, Ishida, S and Tsubota, K 2009. Prevention of ocular inflammation in endotoxin-induced uveitis with resveratrol by inhibiting oxidative damage and nuclear factor-kappa B activation. Investigative Ophthalmology & Visual Science 50, 3512–3519.Google Scholar
Kyrylenko, S and Baniahmad, A 2010. Sirtuin family: a link to metabolic signaling and senescence. Current Medicinal Chemistry 17, 2921–2932.Google Scholar
Lafontaine-Lacasse, M, Richard, D and Picard, F 2010. Effects of age and gender on Sirt 1 mRNA expressions in the hypothalamus of the mouse. Neuroscience Letters 480, 1–3.CrossRefGoogle ScholarPubMed
Li, Y, Xu, W, McBurney, MW and Longo, VD 2008. SirT1 inhibition reduces IGF-I/IRS-2/Ras/ERK1/2 signaling and protects neurons. Cell Metabolism 8, 38–48.Google Scholar
Liszt, G, Ford, E, Kurtev, M and Guarente, L 2005. Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase. Journal of Biological Chemistry 280, 21313–21320.Google Scholar
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method. Methods 25, 402–408.Google Scholar
Lomb, DJ, Laurent, G and Haigis, MC 2010. Sirtuins regulate key aspects of lipid metabolism. Biochimica et Biophysica Acta 1804, 1652–1657.Google Scholar
Mariani, E, Polidori, MC, Cherubini, A and Mecocci, P 2005. Oxidative stress in brain aging, neurodegenerative and vascular diseases: an overview. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 827, 65–75.Google Scholar
Michan, S and Sinclair, D 2007. Sirtuins in mammals: insights into their biological function. The Biochemical Journal 404, 1–13.Google Scholar
Michishita, E, Park, JY, Burneskis, JM, Barrett, JC and Horikawa, I 2005. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins. Molecular Biology of the Cell 16, 4623–4635.Google Scholar
Nakagawa, T and Guarente, L 2011. Sirtuins at a glance. Journal of Cell Science 124, 833–838.Google Scholar
Ozata, M, Mergen, M, Oktenli, C, Aydin, A, Sanisoglu, SY, Bolu, E, Yilmaz, MI, Sayal, A, Isimer, A and Ozdemir, IC 2002. Increased oxidative stress and hypozincemia in male obesity. Clinical Biochemistry 35, 627–631.CrossRefGoogle ScholarPubMed
Rodgers, JT, Lerin, C, Haas, W, Gygi, SP, Spiegelman, BM and Puigserver, P 2005. Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature 434, 113–118.Google Scholar
Sakamoto, J, Miura, T, Shimamoto, K and Horio, Y 2004. Predominant expression of Sir2alpha, an NAD-dependent histone deacetylase, in the embryonic mouse heart and brain. FEBS Letters 556, 281–286.CrossRefGoogle ScholarPubMed
Shan, T, Ren, Y, Liu, Y, Zhu, L and Wang, Y 2010. Breed difference and regulation of the porcine Sirtuin 1 by insulin. Journal of Animal Science 88, 3909–3917.CrossRefGoogle ScholarPubMed
Shan, T, Wang, Y, Wu, T, Liu, C, Guo, J, Zhang, Y, Liu, J and Xu, Z 2009. Porcine sirtuin 1 gene clone, expression pattern, and regulation by resveratrol. Journal of Animal Science 87, 895–904.Google Scholar
Verdin, E, Hirschey, MD, Finley, LW and Haigis, MC 2010. Sirtuin regulation of mitochondria: energy production, apoptosis, and signaling. Trends in Biochemical Sciences 35, 669–675.Google Scholar
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