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The effect of supplementing pony diets with yeast on 1. In vivo and in vitro digestibility, faecal pH and particle size

Published online by Cambridge University Press:  25 June 2020

A. Garber Open the ORCID record for A. Garber [Opens in a new window] ,
P. M. Hastie Open the ORCID record for P. M. Hastie [Opens in a new window] ,
V. Farci ,
L. Bulmer ,
O. Alzahal  and
J. M. D. Murray Open the ORCID record for J. M. D. Murray [Opens in a new window]
A. Garber*
Affiliation:
University of Glasgow, College of Medical, Veterinary and Life Sciences, School of Veterinary Medicine, McCall Building, Bearsden, GlasgowG61 1QH, UK
P. M. Hastie
Affiliation:
University of Glasgow, College of Medical, Veterinary and Life Sciences, School of Veterinary Medicine, McCall Building, Bearsden, GlasgowG61 1QH, UK
V. Farci
Affiliation:
University of Glasgow, College of Medical, Veterinary and Life Sciences, School of Veterinary Medicine, McCall Building, Bearsden, GlasgowG61 1QH, UK
L. Bulmer
Affiliation:
University of Glasgow, College of Medical, Veterinary and Life Sciences, School of Veterinary Medicine, McCall Building, Bearsden, GlasgowG61 1QH, UK
O. Alzahal
Affiliation:
AB Vista, Woodstock Court, Blenheim Road, Marlborough Business Park, Marlborough, WiltshireSN8 4AN, UK
J. M. D. Murray
Affiliation:
University of Glasgow, College of Medical, Veterinary and Life Sciences, School of Veterinary Medicine, McCall Building, Bearsden, GlasgowG61 1QH, UK
*
†E-mail: Anna.Garber@glasgow.ac.uk, anngarber156@gmail.com
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Abstract

Fibre is essential to maintain healthy gut; however, energy demands of performance horses can be too high to be met by forages alone. Yeast may support the function of cellulolytic bacteria to digest fibre. The aim of this work was to determine the effect of an oral supplement (VistaEQ) containing 4% live yeast on the in vitro and in vivo digestibility of high-starch (HS) and high-fibre diets (HF). Eight ponies were used in a 4 × 4 Latin square design consisting of 4- × 19-day periods and four diets: HF, HF + yeast (HFY), HS and HS + yeast (HSY). In vivo apparent digestibility (AD) was estimated using total collection technique, and faecal particle size was measured using NASCO digestive analyser. Faeces from the ponies were subsequently used as an inoculum in ANKOM RF gas production system to assess fermentation kinetics in vitro. Each module contained 1 g of feed substrate DM in the following combinations: 50% grass hay and 50% alfalfa (HF_50 : 50) or concentrate (HS_50 : 50), and 75% grass hay and 25% alfalfa (HF_75 : 25) or concentrate (HS_75 : 25) with or without yeast. Yeast was able to induce more gas production from HF_75 : 25, HS_75 : 25 and HF_50 : 50 feed substrates incubated with respective faecal inoculum base. Yeast did not affect pH in vitro when the substrates were incubated in 50 : 50 ratio, while the pH was higher for HF_75 : 25 incubated with correspondent faecal inoculum compared to HS_75 : 25 and HSY_75 : 25. Yeast had no effects on ADF and CP AD of either diet. Yeast addition increased DM (HF: 0.2%, HS: 0.4%), organic matter (HF: 0.7%, HS: 1.3%), NDF (HF: 0.5%, HS: 1.5%), total detergent fibre (HF: 0.7%; HS: 0.4%) (P < 0.05) and also tended to increase hemicellulose AD (HF: 0.9%, HS: 1.2%) (P < 0.10). Faecal pH in vivo was higher for both HF diets compared to HS diet without yeast supplementation (P < 0.001, HF and HFY: 6.8; HS: 6.6, HSY: 6.7). However, no difference was observed in faecal pH when HSY was compared to both HF diets. Yeast had no effect on the size of the faecal particles (P > 0.05). Yeast increased in vitro gas production, suggesting more energy could be extracted from the feed, and the in vivo AD of some of the nutrients when HF and HS diets were fed.

Keywords

Saccharomyces cerevisiaegas productionhigh-fibrehigh-starchdiet
Type
Research Article
Information
animal , Volume 14 , Issue 12 , December 2020 , pp. 2481 - 2492
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of The Animal Consortium

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