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Effects of in ovo injection of vitamin C on heat shock protein and metabolic genes expression

Published online by Cambridge University Press:  30 September 2019

Y. F. Zhu ,
M. B. Bodinga ,
J. H. Zhou ,
L. Q. Zhu ,
Y. L. Cao ,
Z. Z. Ren  and
X. J. Yang Open the ORCID record for X. J. Yang [Opens in a new window]
Y. F. Zhu
Affiliation:
Innovative Research Team of Animal Nutrition and Healthy Feeding, College of Animal Science and Technology, Northwest A&F University, No. 22, Xinong Road, Yangling 712100, People’s Republic of China
M. B. Bodinga
Affiliation:
Innovative Research Team of Animal Nutrition and Healthy Feeding, College of Animal Science and Technology, Northwest A&F University, No. 22, Xinong Road, Yangling 712100, People’s Republic of China
J. H. Zhou
Affiliation:
Innovative Research Team of Animal Nutrition and Healthy Feeding, College of Animal Science and Technology, Northwest A&F University, No. 22, Xinong Road, Yangling 712100, People’s Republic of China
L. Q. Zhu
Affiliation:
Innovative Research Team of Animal Nutrition and Healthy Feeding, College of Animal Science and Technology, Northwest A&F University, No. 22, Xinong Road, Yangling 712100, People’s Republic of China
Y. L. Cao
Affiliation:
Innovative Research Team of Animal Nutrition and Healthy Feeding, College of Animal Science and Technology, Northwest A&F University, No. 22, Xinong Road, Yangling 712100, People’s Republic of China
Z. Z. Ren
Affiliation:
Innovative Research Team of Animal Nutrition and Healthy Feeding, College of Animal Science and Technology, Northwest A&F University, No. 22, Xinong Road, Yangling 712100, People’s Republic of China
X. J. Yang*
Affiliation:
Innovative Research Team of Animal Nutrition and Healthy Feeding, College of Animal Science and Technology, Northwest A&F University, No. 22, Xinong Road, Yangling 712100, People’s Republic of China
*
†E-mail: yangxj@nwsuaf.edu.cn
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Abstract

Some studies have shown that the excessive metabolic heat production is the primary cause for dead chicken embryos during late embryonic development. Increasing heat shock protein (HSP) expression and adjusting metabolism are important ways to maintain body homeostasis under heat stress. This study was conducted to investigate the effects of in ovo injection (IOI) of vitamin C (VC) at embryonic age 11th day (E11) on HSP and metabolic genes expression. A total of 320 breeder eggs were randomly divided into normal saline and VC injection groups. We detected plasma VC content and rectal temperature at chick’s age 1st day, and the mRNA levels of HSP and metabolic genes in embryonic livers at E14, 16 and 18, analysed the promoter methylation levels of differentially expressed genes and predicted transcription factors at the promoter regions. The results showed that IOI of VC significantly increased plasma VC content and decreased rectal temperature (P < 0.05). In ovo injection of VC significantly increased heat shock protein 60 (HSP60) and pyruvate dehydrogenase kinase 4 (PDK4) genes expression at E16 and PDK4 and secreted frizzled related protein 1 (SFRP1) at E18 (P < 0.05). At E16, IOI of VC significantly decreased the methylation levels of total CpG sites and −336 CpG site in HSP60 promoter and −1137 CpG site in PDK4 promoter (P < 0.05). Potential binding sites for nuclear factor-1 were found around −389 and −336 CpG sites in HSP60 promoter and potential binding site for specificity protein 1 was found around −1137 CpG site in PDK4 promoter. Our results suggested that IOI of VC increased HSP60, PDK4 and SFRP1 genes expression at E16 and 18, which may be associated with the demethylation in gene promoters. Whether IOI of VC could improve hatchability needs to be further verified by setting uninjection group.

Keywords

ascorbic acidchicken embryoin ovo feedingpromoterDNA demethylation
Type
Research Article
Information
animal , Volume 14 , Issue 2 , February 2020 , pp. 360 - 367
Copyright
© The Animal Consortium 2019 

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Footnotes

a

These authors contributed equally to this work.

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