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Effects of low-protein diets supplemented with indispensable amino acids on growth performance, intestinal morphology and immunological parameters in 13 to 35 kg pigs

Published online by Cambridge University Press:  23 May 2016

X. Peng ,
L. Hu ,
Y. Liu ,
C. Yan ,
Z. F. Fang ,
Y. Lin ,
S. Y. Xu ,
J. Li ,
C. M. Wu  and
D. W. Chen
...Show all authors
X. Peng
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
L. Hu
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
Y. Liu
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
C. Yan
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
Z. F. Fang
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
Y. Lin
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
S. Y. Xu
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
J. Li
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
C. M. Wu
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
D. W. Chen
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
H. Sun
Affiliation:
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130033, Jilin, P. R. China
D. Wu
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
L. Q. Che*
Affiliation:
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, P. R. China
*
†E-mail: clianqiang@hotmail.com
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Abstract

The objective of this study was to determine if a moderate or high reduction of dietary CP, supplemented with indispensable amino acids (IAA), would affect growth, intestinal morphology and immunological parameters of pigs. A total of 40 barrows (initial BW=13.50±0.50 kg, 45±2 day of age) were used in a completely randomized block design, and allocated to four dietary treatments containing CP levels at 20.00%, 17.16%, 15.30% and 13.90%, respectively. Industrial AA were added to meet the IAA requirements of pigs. After 4-week feeding, blood and tissue samples were obtained from pigs. The results showed that reducing dietary CP level decreased average daily gain, plasma urea nitrogen concentration and relative organ weights of liver and pancreas (P<0.01), and increased feed conversion ratio (P<0.01). Pigs fed the 13.90% CP diet had significantly lower growth performance than that of pigs fed higher CP at 20.00%, 17.16% or 15.30%. Moreover, reducing dietary CP level decreased villous height in duodenum (P<0.01) and crypt depth in duodenum, jejunum and ileum (P<0.01). The reduction in the dietary CP level increased plasma concentrations of methionine, alanine (P<0.01) and lysine (P<0.05), and decreased arginine (P<0.05). Intriguingly, reducing dietary CP level from 20.00% to 13.90% resulted in a significant decrease in plasma concentration of IgG (P<0.05), percentage of CD3+T cells of the peripheral blood (P<0.01), also down-regulated the mRNA abundance of innate immunity-related genes on toll-like receptor 4, myeloid differentiation factor 88 (P<0.01) and nuclear factor kappa B (P<0.05) in the ileum. These results indicate that reducing dietary CP level from 20.00% to 15.30%, supplemented with IAA, had no significant effect on growth performance and had a limited effect on immunological parameters. However, a further reduction of dietary CP level up to 13.90% would lead to poor growth performance and organ development, associated with the modifications of intestinal morphology and immune function.

Keywords

amino acidsprotein levelimmune functionintestinal morphologypig
Type
Research Article
Information
animal , Volume 10 , Issue 11 , November 2016 , pp. 1812 - 1820
Copyright
© The Animal Consortium 2016 

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References

Association of Official Analytical Chemists (AOAC) 2003. Official methods of analysis, 17th edition. AOAC, Arlington, Virginia.Google Scholar
Boza, J, Moennoz, D, Vuichoud, J, Jarret, A, Gaudard-de-Weck, D and Ballevre, O 2000. Protein hydrolysate vs free amino acid-based diets on the nutritional recovery of the starved rat. European Journal of Nutrition 39, 237–243.CrossRefGoogle ScholarPubMed
Columbus, D, Lapierre, H, Fuller, M, Htoo, J and de Lange, C 2012. The impact of lower gut nitrogen supply on nitrogen balance and urea kinetics in growing pigs fed a valine-limiting diet. Journal of Animal Science 90, 62–64.CrossRefGoogle ScholarPubMed
Deng, D, Huang, RL, Li, TJ, Wu, GY, Xie, MY, Tang, ZR, Kang, P, Zhang, YM, Fan, MZ, Kong, XF, Ruan, Z, Xiong, H, Deng, ZY and Yin, YL 2007. Nitrogen balance in barrows fed low-protein diets supplemented with essential amino acids. Livestock Science 109, 220–223.CrossRefGoogle Scholar
Deng, Y, Cui, H, Peng, X, Fang, J, Wang, K, Cui, W and Liu, X 2011. Effect of dietary vanadium on cecal tonsil T cell subsets and IL-2 contents in broilers. Biological Trace Element Research 144, 647–656.CrossRefGoogle ScholarPubMed
Figueroa, J, Lewis, A, Miller, P, Fischer, R and Diedrichsen, R 2003. Growth, carcass traits, and plasma amino acid concentrations of gilts fed low-protein diets supplemented with amino acids including histidine, isoleucine, and valine. Journal of Animal Science 81, 1529–1537.CrossRefGoogle ScholarPubMed
Figueroa, J, Lewis, A, Miller, P, Fischer, R, Gómez, R and Diedrichsen, R 2002. Nitrogen metabolism and growth performance of gilts fed standard corn-soybean meal diets or low-crude protein, amino acid-supplemented diets. Journal of Animal Science 80, 2911–2919.CrossRefGoogle ScholarPubMed
Gloaguen, M, Le Floc’h, N, Corrent, E, Primot, Y and van Milgen, J 2014. The use of free amino acids allows formulating very low crude protein diets for piglets. Journal of Animal Science 92, 637–644.CrossRefGoogle ScholarPubMed
Gu, X and Li, D 2004. Effect of dietary crude protein level on villous morphology, immune status and histochemistry parameters of digestive tract in weaning piglets. Animal Feed Science and Technology 114, 113–126.CrossRefGoogle Scholar
Guay, F, Donovan, SM and Trottier, NL 2006. Biochemical and morphological developments are partially impaired in intestinal mucosa from growing pigs fed reduced-protein diets supplemented with crystalline amino acids. Journal of Animal Science 84, 1749–1760.CrossRefGoogle ScholarPubMed
Guay, F and Trottier, NL 2006. Muscle growth and plasma concentrations of amino acids, insulin-like growth factor-I, and insulin in growing pigs fed reduced-protein diets. Journal of Animal Science 84, 3010–3019.CrossRefGoogle ScholarPubMed
Heo, JM, Kim, JC, Hansen, CF, Mullan, BP, Hampson, DJ and Pluske, JR 2010. Effects of dietary protein level and zinc oxide supplementation on performance responses and gastrointestinal tract characteristics in weaner pigs challenged with an enterotoxigenic strain of Escherichia coli . Animal Production Science 50, 827–836.CrossRefGoogle Scholar
Hu, L, Liu, Y, Yan, C, Peng, X, Xu, Q, Xuan, Y, Han, F, Tian, G, Fang, Z, Lin, Y, Xu, S, Zhang, K, Chen, D, Wu, D and Che, L 2015. Postnatal nutritional restriction affects growth and immune function of piglets with intra-uterine growth restriction. British Journal of Nutrition 114, 53–62.CrossRefGoogle ScholarPubMed
Kawai, T and Akira, S 2009. The roles of TLRs, RLRs and NLRs in pathogen recognition ARTICLE. International Immunology 21, 317–337.CrossRefGoogle Scholar
Kerr, B, McKeith, F and Easter, R 1995. Effect on performance and carcass characteristics of nursery to finisher pigs fed reduced crude protein, amino acid-supplemented diets. Journal of Animal Science 73, 433–440.CrossRefGoogle ScholarPubMed
Kerr, B, Yen, J, Nienaber, J and Easter, R 2003. Influences of dietary protein level, amino acid supplementation and environmental temperature on performance, body composition, organ weights and total heat production of growing pigs. Journal of Animal Science 81, 1998–2007.CrossRefGoogle ScholarPubMed
Kong, XF, Yin, YL, He, QH, Yin, FG, Liu, HJ, Li, TJ, Huang, RL, Geng, MM, Ruan, Z, Deng, ZY, Xie, MY and Wu, G 2009. Dietary supplementation with Chinese herbal powder enhances ileal digestibilities and serum concentrations of amino acids in young pigs. Amino acids 37, 573–582.CrossRefGoogle ScholarPubMed
Lenis, NP, van Diepen, H, Bikker, P, Jongbloed, AW and van der Meulen, J 1999. Effect of the ratio between essential and nonessential amino acids in the diet on utilization of nitrogen and amino acids by growing pigs. Journal of Animal Science 77, 1777–1787.CrossRefGoogle ScholarPubMed
Lin, C-m, Abcouwer, SF and Souba, WW 1999. Effect of dietary glutamate on chemotherapy-induced immunosuppression. Nutrition 15, 687–696.CrossRefGoogle ScholarPubMed
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25, 402–408.CrossRefGoogle Scholar
McCleary, B, Solah, V and Gibson, T 1994. Quantitative measurement of total starch in cereal flours and products. Journal of Cereal Science 20, 51–58.CrossRefGoogle Scholar
National Research Council (NRC) 2012. Nutrient requirements of swine, 11th edition. National Academy Press, Washington, DC, USA.Google Scholar
Noblet, J, Fortune, H, Shi, XS and Dubois, S 1994. Prediction of net energy value of feeds for growing pigs. Journal of Animal Science 72, 344–354.CrossRefGoogle ScholarPubMed
Nyachoti, C, Omogbenigun, F, Rademacher, M and Blank, G 2006. Performance responses and indicators of gastrointestinal health in early-weaned pigs fed low-protein amino acid-supplemented diets. Journal of Animal Science 84, 125–134.CrossRefGoogle ScholarPubMed
Opapeju, FO, Krause, DO, Payne, RL, Rademacher, M and Nyachoti, CM 2009. Effect of dietary protein level on growth performance, indicators of enteric health, and gastrointestinal microbial ecology of weaned pigs induced with postweaning colibacillosis. Journal of Animal Science 87, 2635–2643.CrossRefGoogle ScholarPubMed
Opapeju, FO, Rademacher, M, Blank, G and Nyachoti, CM 2008. Effect of low-protein amino acid-supplemented diets on the growth performance, gut morphology, organ weights and digesta characteristics of weaned pigs. Animal 2, 1457–1464.CrossRefGoogle ScholarPubMed
Powell, S, Bidner, TD, Payne, RL and Southern, LL 2011. Growth performance of 20-to 50-kilogram pigs fed low-crude-protein diets supplemented with histidine, cystine, glycine, glutamic acid, or arginine. Journal of Animal Science 89, 3643–3650.CrossRefGoogle ScholarPubMed
Ruth, MR and Field, CJ 2013. The immune modifying effects of amino acids on gut-associated lymphoid tissue. Journal of Animal Science and Biotechnology 4, 27.CrossRefGoogle ScholarPubMed
Uematsu, S and Akira, S 2006. Toll-like receptors and innate immunity. Journal of Molecular Medicine 84, 712–725.CrossRefGoogle ScholarPubMed
Van Soest, Pv, Robertson, J and Lewis, B 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.CrossRefGoogle ScholarPubMed
Wu, G 1997. Synthesis of citrulline and arginine from porcine enterocytes of postnatal pigs. American Journal of Physiology-Gastrointestinal and Liver Physiology 272, G1382–G1390.CrossRefGoogle ScholarPubMed
Wu, G 1998. Intestinal mucosal amino acid catabolism. The Journal of Nutrition 128, 1249–1252.CrossRefGoogle ScholarPubMed
Wu, G 2014. Dietary requirements of synthesizable amino acids by animals: a paradigm shift in protein nutrition. Journal of Animal Science and Biotechnology 5, 34.CrossRefGoogle ScholarPubMed
Xia, MS, Hu, CH and Xu, ZR 2005. Effects of copper bearing montmorillonite on the growth performance, intestinal microflora and morphology of weanling pigs. Animal Feed Science and Technology 118, 307–317.CrossRefGoogle Scholar
Yue, LY and Qiao, SY 2008. Effects of low-protein diets supplemented with crystalline amino acids on performance and intestinal development in piglets over the first 2 weeks after weaning. Livestock Science 115, 144–152.CrossRefGoogle Scholar