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Effects of protein sources on concentrations of hydrogen sulphide in the rumen headspace gas of dairy cows

Published online by Cambridge University Press:  15 May 2012

A. J. M. Fonseca ,
A. R. J. Cabrita ,
L. A. O. Pinho ,
E. J. Kim  and
R. J. Dewhurst
A. J. M. Fonseca
Affiliation:
REQUIMTE, ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão VC, Portugal
A. R. J. Cabrita
Affiliation:
REQUIMTE, Departamento de Geociências, Ambiente e Ordenamento do Território, Faculdade de Ciências, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão VC, Portugal
L. A. O. Pinho
Affiliation:
REQUIMTE, ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão VC, Portugal
E. J. Kim
Affiliation:
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth SY23 3EB, United Kingdom
R. J. Dewhurst*
Affiliation:
Teagasc, Animal and Grassland Research and Innovation Centre, Grange, Dunsany, County Meath, Ireland
*
†E-mail: Richard.Dewhurst@teagasc.ie
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Abstract

Two Latin square design experiments investigated the relationship between hydrogen sulphide concentration in the rumen headspace gas of dairy cows and the early stages of protein degradation in the rumen. In Expt 1, three protein sources differing in rumen N (nitrogen) degradability (maize gluten feed (MGF); sunflower meal (SFM); and soyabean meal (SBM)) were used, whereas in Expt 2 four different batches of the same feed (MGF) differing in colour (CIE L*, a*, b* (CIELAB) scale) were used. After allowing the concentration of hydrogen sulphide in rumen gas to decline close to zero, a fixed amount of protein sources was offered to cows and the concentrations of hydrogen sulphide were recorded in rumen headspace gas at 30-min intervals. In Expt 1, the concentration of hydrogen sulphide showed considerable variation between protein sources, with MGF having the highest concentration followed by SFM and SBM resulting in very low concentrations. The N wash losses (zero time measurements with nylon bags) ranked the feeds in the same way, from MGF (highest; 61%) to SBM (lowest; 26%). There were marked differences in the degradation of cystine and methionine between protein sources, although the degradation of cystine was always higher than for methionine. MGF (Expt 2) led to increased concentrations of hydrogen sulphide, with peak concentrations achieved between 1 and 2 h after feeding. The concentrations of hydrogen sulphide were higher for MGF1, intermediate for MGF2 and lower for MGF3 and MGF4, agreeing with colour scale. Differences in the early stages of dietary sulphur degradation corresponded with differences in hydrogen sulphide concentrations in rumen gas. The results suggest that hydrogen sulphide concentrations in the rumen headspace gas could be useful to evaluate nutritional parameters not measured by the in sacco technique, contributing to a better understanding of the response of dairy cows to different protein supplements.

Keywords

dairy cowhydrogen sulphiderumenprotein sources
Type
Nutrition
Information
animal , Volume 7 , Issue 1 , January 2013 , pp. 75 - 81
Copyright
Copyright © The Animal Consortium 2012

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References

Agricultural and Food Research Council (AFRC) 1993. Energy and protein requirements of ruminants. An advisory manual prepared by the AFRC Technical Committee on Responses to Nutrients, 159 pp.CAB International, Wallingford, UK.Google Scholar
Anderson, CM 1956. The metabolism of sulphur in the rumen of the sheep. New Zealand Journal of Science and Technology 37, 379–394.Google Scholar
Bach, A, Ruiz Moreno, M, Thrune, M, Stern, MD 2008. Evaluation of the fermentation dynamics of soluble crude protein from three protein sources in continuous culture fermenters. Journal of Animal Science 86, 1364–1371.CrossRefGoogle ScholarPubMed
Bird, PR 1972. Sulphur metabolism and excretion studies in ruminants. V. Ruminal desulphuration of methionine and cyst(e)ine. Australian Journal of Biological Science 25, 185–193.CrossRefGoogle ScholarPubMed
Cabrita, ARJ, Dewhurst, RJ, Melo, DSP, Moorby, JM, Fonseca, AJM 2011a. Effects of dietary protein concentration and balance of absorbable amino acids on productive responses of dairy cows fed corn silage-based diets. Journal of Dairy Science 94, 4647–4656.Google Scholar
Cabrita, ARJ, Maia, MRG, Freitas, M, Abreu, JMF, Fonseca, AJM 2011b. Colour score as a guide for estimating the protein value of corn gluten feed. Journal of the Science of Food and Agriculture 91, 1648–1652.Google Scholar
Dewhurst, RJ, Hepper, D, Webster, AJF 1995. Comparison of in sacco and in vitro techniques for estimating the site and extent of rumen fermentation of a range of diet ingredients. Animal Feed Science and Technology 51, 211–229.CrossRefGoogle Scholar
Dewhurst, RJ, Moorby, JM, Dhanoa, MS, Evans, RT, Fisher, WJ 2000. Effects of altering energy and protein supply to dairy cows during the dry period. 1. Intake, body condition and milk production. Journal of Dairy Science 83, 1782–1794.CrossRefGoogle ScholarPubMed
Dewhurst, RJ, Evans, RT, Mottram, TT, Španěl, P, Smith, D 2001. Assessment of rumen processes by selected-ion-flow-tube mass spectrometric analysis of rumen gases. Journal of Dairy Science 84, 1438–1444.CrossRefGoogle ScholarPubMed
Dewhurst, RJ, Kim, EJ, Evans, RT, Cabrita, ARJ, Fonseca, AJM 2007. Effects of dietary sulphur sources on concentrations of hydrogen sulphide in the rumen head-space gas of dairy cows. Animal 1, 531–535.Google Scholar
Fundación Española para el Desarrollo de la Nutrición Animal (FEDNA), 1999. Normas FEDNA para la fomrulación de peinsos compuestos, Madrid, 496 p.Google Scholar
Gill, M, Robinson, PH, Kennelly, JJ 1999. Diurnal patterns in rumen volume and composition of digesta flowing out of the duodenum. Animal Science 69, 237–249.Google Scholar
Guedes, CM, Dias-da-Silva, A 2005. Effects of dietary carbohydrate sources on degradation kinetics of nitrogen from plant protein sources in the rumen. Journal of the Science of Food and Agriculture 85, 1795–1804.CrossRefGoogle Scholar
Halverson, AW, Williams, GD, Paulson, GD 1968. Aspects of sulfate utilization by the microorganisms of the ovine rumen. Journal of Nutrition 95, 363–368.CrossRefGoogle ScholarPubMed
Havlin, JL, Tisdale, SL, Nelson, W, Beaton, J 1999. Soil fertility and fertilisers: an introduction to nutrient management, 6th edition. Prentice-Hall, Upper Saddle River, New Jersey, USA.Google Scholar
Kandylis, K 1983. Transfer of plasma sulfate from blood to rumen. A review. Journal of Dairy Science 66, 2263–2270.CrossRefGoogle ScholarPubMed
Leibovich, J, Vasconcelos, JT, Galyean, ML 2009. Effects of corn processing method in diets containing sorghum wet distillers grain plus solubles on performance and carcass characteristics of finishing beef cattle and on in vitro fermentation of diets. Journal of Animal Science 87, 2124–2132.Google Scholar
Lewis, D 1955. Amino acid metabolism in the rumen of the sheep. British Journal of Nutrition 9, 215–230.Google Scholar
Ministry of Agriculture, Fisheries and Food 1986. Reference book 427: the analysis of agricultural materials, 3rd edition. Her Majesty's Stationery Office, London.Google Scholar
Ørskov, ER, Hovell, FDDeB, Mould, F 1980. The use of nylon bag technique for the evaluation of feedstuffs. Tropical Animal Production 5, 195–213.Google Scholar
Quinn, MJ, May, ML, Hales, KE, DiLorenzo, N, Leibovich, J, Smith, DR, Galyean, ML 2009. Effects of ionophores and antibiotics on in vitro hydrogen sulfide production, dry matter disappearance, and total gas production in cultures with a steam-flaked corn-based substrate with or without added sulphur. Journal of Animal Science 87, 1705–1713.CrossRefGoogle ScholarPubMed
Smith, DR, DiLorenzo, N, Leibovich, J, May, ML, Quinn, MJ, Homm, JW, Galyean, ML 2010. Effects of sulfur and monensin concentrations on in vitro dry matter disappearance, hydrogen sulfide production, and volatile fatty acid concentrations in batch culture ruminal fermentations. Journal of Animal Science 88, 1503–1512.CrossRefGoogle ScholarPubMed