Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-26T17:40:13.740Z Has data issue: false hasContentIssue false
  • English
  • Français

Estrous expression during a fixed-time artificial insemination protocol enhances development and interferon-tau messenger RNA expression in conceptuses from Bos indicus beef cows

Published online by Cambridge University Press:  10 April 2019

R. F. Cooke Open the ORCID record for R. F. Cooke [Opens in a new window] ,
K. G. Pohler ,
J. L. M. Vasconcelos  and
R. L. A. Cerri
R. F. Cooke*
Affiliation:
Department of Animal Science, Texas A&M University,College Station, TX77845, USA
K. G. Pohler
Affiliation:
Department of Animal Science, Texas A&M University,College Station, TX77845, USA
J. L. M. Vasconcelos
Affiliation:
School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18168-000, Brazil
R. L. A. Cerri
Affiliation:
Faculty of Land and Food Systems, University of British Columbia,Vancouver, BC, CanadaV6T 1Z4
*
†E-mail: reinaldocooke@tamu.edu
Get access

Abstract

Expression of estrus (EST) near the time of fixed-time artificial insemination (FTAI) increases pregnancy success in beef females. This outcome has been associated with improved pregnancy establishment and maintenance, although research is still warranted to validate this theory. Hence, this experiment compared ovarian, uterine and conceptus factors associated with pregnancy establishment in Bos indicus beef cows according to estrous expression during a FTAI protocol. One hundred lactating multiparous Nelore cows received a 2 mg injection of estradiol benzoate and an intravaginal progesterone (P4) releasing device on day −11, a 12.5 mg injection of prostaglandin F2α on day −4, P4 device removal in addition to 0.6 mg injection of estradiol cypionate and 300 IU injection of equine chorionic gonadotropin on day −2, and FTAI on day 0. An estrous detection patch was attached to the tailhead of each cow on day −2, and estrous expression was defined as removal of >50% of the rub-off coating from the patch at FTAI. Overall, 39 cows expressed EST, 55 did not express EST (NOEST), and six cows lost their patch and were discarded from the experiment. Ovarian ultrasonography was performed at FTAI, and on days 7 and 15 of the experiment. Blood samples were also collected on days 7 and 15. Only cows without a corpus luteum (CL) on day 0, and with a CL on days 7 and 15 remained in the experiment (EST, n=36; NOEST, n=48). On day 15, cows were randomly selected within each group (EST, n=29; NOEST, n=30) for conceptus collection via transcervical flushing, followed by endometrial biopsy in the uterine horn ipsilateral to the CL. Within cows not assigned to conceptus collection, blood samples were collected for whole blood RNA extraction (day 20) and pregnancy status was verified by transrectal ultrasonography (day 30). Diameter of dominant follicle on day 0 and plasma P4 concentrations on day 7 were greater (P⩽0.02) in EST v. NOEST cows. Conceptus length and messenger RNA (mRNA) expression of prostaglandin E synthase and interferon-tau were greater (P⩽0.04) in EST v. NOEST cows. Moreover, EST cows diagnosed as pregnant on day 30 had greater (P<0.01) blood mRNA expression of myxovirus resistance 2 on day 20 compared with NOEST. In summary, estrous expression near the time of FTAI enhanced pregnancy establishment factors in B. indicus cows, including conceptus development and mRNA expression of interferon-tau.

Keywords

beef cattleinseminationphysiologypregnancyreproduction
Type
Research Article
Information
animal , Volume 13 , Issue 11 , November 2019 , pp. 2569 - 2575
Copyright
© The Animal Consortium 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allrich, RD 1994. Endocrine and neural control of estrus in dairy cows. Journal of Dairy Science 77, 2738–2744.CrossRefGoogle ScholarPubMed
Arosh, JA, Parent, J, Chapdelaine, P, Sirois, J and Fortier, MA 2002. Expression of cyclooxygenases 1 and 2 and prostaglandin E synthase in bovine endometrial tissue during the estrous cycle. Biology of Reproduction 67, 161–169.CrossRefGoogle ScholarPubMed
Brandão, AP, Cooke, RF, Schubach, KM, Marques, RS, Bohnert, DW, Carvalho, RS, Dias, NW, Timlin, CL, Clark-Deener, S, Currin, JF, Jump, DB, Pohler, KG, Cerri, RLA and Mercadante, VRG 2018. Supplementing Ca salts of soybean oil after artificial insemination increases pregnancy success in Bos taurus beef cows. Journal of Animal Science 96, 2838–2850.CrossRefGoogle ScholarPubMed
Brooks, K, Burns, G and Spencer, TE 2014. Conceptus elongation in ruminants: roles of progesterone, prostaglandin, interferon tau and cortisol. Journal of Animal Science and Biotechnology 5, 53.CrossRefGoogle ScholarPubMed
Buhi, WC 2002. Characterization and biological roles of oviduct-specific, oestrogen-dependent glycoprotein. Reproduction 123, 355–362.CrossRefGoogle ScholarPubMed
Carvalho, ER, Martins, T, Lamb, GC and Vasconcelos, JLM 2016. Ovulation time in suckled beef cows is anticipated by use of low doses of progesterone and temporary calf removal on fixed timed AI protocol. Theriogenology 86, 2238–2243.CrossRefGoogle ScholarPubMed
Cerri, RLA, Chebel, RC, Rivera, F, Narciso, CD, Oliveira, RA, Amstalden, M, Baez-Sandoval, GM, Oliveira, LJ, Thatcher, WW and Santos, JEP 2011. Concentration of progesterone during the development of the ovulatory follicle: II. Ovarian and uterine responses. Journal of Dairy Science 94, 3352–3365.CrossRefGoogle ScholarPubMed
Cipriano, RS, Cooke, RF, Rodrigues, AD, Silva, LGT, Bohnert, DW, Marques, RS, Vasconcelos, JLM, Pires, AV and Cerri, RLA 2016. Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows. Journal of Animal Science 94, 4892–4902.CrossRefGoogle Scholar
Cooke, RF, Arthington, JD, Araujo, DB, Lamb, GC and Ealy, AD 2008. Effects of supplementation frequency on performance, reproductive, and metabolic responses of Brahman-crossbred females. Journal of Animal Science 86, 2296–2309.CrossRefGoogle ScholarPubMed
Cooke, RF, Cappellozza, BI, Guarnieri Filho, TA, Depner, CM, Lytle, KA, Jump, DB, Bohnert, DW, Cerri, RLA and Vasconcelos, JLM 2014. Effects of calcium salts of soybean oil on factors that influence pregnancy establishment in Bos indicus beef cows. Journal of Animal Science 92, 2239–2250.CrossRefGoogle ScholarPubMed
Davoodi, S, Cooke, RF, Fernandes, ACC, Cappellozza, BI, Vasconcelos, JLM and Cerri, TLA 2016. Expression of estrus modifies the gene expression profile in reproductive tissues on day 19 of gestation in beef cows. Theriogenology 85, 645–655.CrossRefGoogle Scholar
Demetrio, DG, Santos, RM, Demetrio, CG and Vasconcelos, JL 2007. Factors affecting conception rates following artificial insemination or embryo transfer in lactating Holstein cows. Journal of Dairy Science 90, 5073–5082.CrossRefGoogle ScholarPubMed
Ealy, AD and Yang, QE 2009. Control of interferon‐tau expression during early pregnancy in ruminants. American Journal of Reproductive Immunology 61, 95–106.CrossRefGoogle ScholarPubMed
Figueiredo, RA, Barros, CM, Pinheiro, OL and Soler, JMP 1997. Ovarian follicular dynamics in Nelore breed (Bos indicus) cattle. Theriogenology 47, 1489–1505.CrossRefGoogle ScholarPubMed
Fleige, S and Pfaffl, MW 2006. RNA integrity and the effect on the real-time qRT-PCR performance. Molecular Aspects of Medicine 27, 126–139.CrossRefGoogle ScholarPubMed
Food and Agricultural Organization (FAO) 2009. How to feed the world in 2050. In: Proceedings of the Expert Meeting on How to Feed the World in 2050, 24–26 June 2009. FAO Headquarters, Rome, Italy.Google Scholar
Forde, N, Carter, F, Fair, T, Crowe, MA, Evans, AC, Spencer, TE, Bazer, FW, McBride, R, Boland, MP, O’gaora, P and Lonergan, P 2009. Progesterone-regulated changes in endometrial gene expression contribute to advanced conceptus development in cattle. Biology of Reproduction 81, 784–794.CrossRefGoogle ScholarPubMed
Forde, N, McGettigan, PA, Mehta, JP, O’Hara, L, Mamo, S, Bazer, FW, Spencer, T and Lonergan, P 2014. Proteomic analysis of uterine fluid during the pre-implantation period of pregnancy in cattle. Reproduction 29, REP-13.Google Scholar
Fricke, PM, Carvalho, PD, Lucy, MC, Curran, F, Herlihy, MM, Waters, SM, Larkin, JA, Crowe, MA and Butler, ST 2016. Effect of manipulating progesterone before timed artificial insemination on reproductive and endocrine parameters in seasonal-calving, pasture-based Holstein-Friesian cows. Journal of Dairy Science 99, 6780–6792.CrossRefGoogle ScholarPubMed
Gray, CA, Taylor, KM, Ramsey, WS, Hill, JR, Bazer, FW, Bartol, FF and Spencer, TE 2001. Endometrial glands are required for preimplantation conceptus elongation and survival. Biology of Reproduction 64, 1608–1613.CrossRefGoogle Scholar
Lamb, GC, Dahlen, CR, Larson, JE, Marquezini, G and Stevenson, JS 2010. Control of the estrous cycle to improve fertility for fixed-time artificial insemination in beef cattle: a review. Journal of Animal Science 88, E181–E192.CrossRefGoogle ScholarPubMed
Larimore, EL, Amundson, OL, Bird, SL, Funnell, BJ, Kruse, SG, Bridges, GA and Perry, GA 2015. Influence of estrus at fixed-time artificial insemination on early 565 embryonic development in beef cattle. Journal of Animal Science 93, 2806–2812.CrossRefGoogle Scholar
Meneghetti, M, Sa Filho, OG, Peres, RFC, Lamb, GC and Vasconcelos, JLM 2009. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cattle: I. Basis for development of protocols. Theriogenology 72, 179–189.CrossRefGoogle ScholarPubMed
Murdoch, WJ and Van Kirk, EA 1998. Luteal dysfunction in ewes induced to ovulate early in the follicular phase. Endocrinology 139, 3480–3484.CrossRefGoogle ScholarPubMed
Patterson, DJ, Cooke, RF, Dahlke, GR, Funston, RN, Hall, JB, Lamb, GC, Lauderdale, J, Perry, GA and Van Eenennaam, AL 2016. Physiological and management advances enhancing adoption of applied reproductive management procedures in beef cattle. Journal of Animal Science 94, 550.CrossRefGoogle Scholar
Pereira, MH, Sanches, CP, Guida, TG, Rodrigues, AD, Aragon, FL, Veras, MB, Borges, PT, Wiltbank, MC and Vasconcelos, JLM 2013. Timing of prostaglandin F2α treatment in an estrogen-based protocol for timed artificial insemination or timed embryo transfer in lactating dairy cows. Journal of Dairy Science 96, 2837–2846.CrossRefGoogle ScholarPubMed
Perry, GA, Smith, MF, Lucy, MC, Green, JA, Parks, TE, MacNeil, MD, Roberts, AJ and Geary, TW 2005. Relationship between follicle size at insemination and pregnancy success. Proceedings of the National Academy of Sciences of the United States of America 102, 5268–5273.CrossRefGoogle ScholarPubMed
Pohler, KG, Geary, TW, Atkins, JA, Perry, GA, Jinks, EM and Smith, MF 2012. Follicular determinants of pregnancy establishment and maintenance. Cell Tissue Research 349, 649–664.CrossRefGoogle ScholarPubMed
Ribeiro, ES, Monteiro, APA, Bisinotto, RS, Lima, FS, Greco, LF, Ealy, AD, Thatcher, WW and Santos, JEP 2016. Conceptus development and transcriptome at preimplantation stages in lactating dairy cows of distinct genetic groups and estrous cyclic statuses. Journal of Dairy Science 99, 4761–4777.CrossRefGoogle ScholarPubMed
Rodrigues, AD, Cooke, RF, Cipriano, RS, Silva, LG, Cerri, RL, Cruppe, LH, Meneghetti, M, Pohler, KG and Vasconcelos, JLM 2018. Impacts of estrus expression and intensity during a timed-AI protocol on variables associated with fertility and pregnancy success in Bos indicus-influenced beef cows. Journal of Animal Science 96, 236–249.CrossRefGoogle ScholarPubMed
Schubach, KM, Cooke, R, Brandao, AP, Marques, R and Cerri, RLA. 2018. Impacts of estrus expression during a timed-AI protocol on variables associated with fertility and pregnancy success in Bos indicus beef cows. Journal of Animal Science 96 (suppl. 1), 51.CrossRefGoogle Scholar
Stevenson, JL, Dalton, JC, Ott, TL, Racicot, KE and Chebel, RC 2007. Correlation between reproductive status and steady-state messenger ribonucleic acid levels of the Myxovirus resistance gene, MX2, in peripheral blood leukocytes of dairy heifers. Journal of Animal Science 85, 2163–2172.CrossRefGoogle ScholarPubMed
Vasconcelos, JL, Sartori, R, Oliveira, HN, Guenther, JG and Wiltbank, MC 2001. Reduction in size of the ovulatory follicle reduces subsequent luteal size and pregnancy rate. Theriogenology 56, 307–314.CrossRefGoogle ScholarPubMed
Vasconcelos, JLM, de Sá Filho, OG and Cooke, RF 2014. Impacts of reproductive technologies on beef production in South America. Advances in Experimental Medicine and Biology 752, pp. 161–180.CrossRefGoogle Scholar
Wagner, JJ, Lusby, KS, Oltjen, JW, Rakestraw, J, Wettemann, RP and Walters, LW 1988. Carcass composition in mature Hereford cows: estimation and effect on daily metabolizable energy requirement during winter. Journal of Animal Science 66, 603–612.CrossRefGoogle ScholarPubMed