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Evaluation of in situ valine production by Bacillus subtilis in young pigs

Published online by Cambridge University Press:  02 May 2016

J. V. Nørgaard ,
N. Canibe ,
E. A. Soumeh ,
B. B. Jensen ,
B. Nielsen ,
P. Derkx ,
M. D. Cantor ,
K. Blaabjerg  and
H. D. Poulsen
J. V. Nørgaard*
Affiliation:
Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark
N. Canibe
Affiliation:
Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark
E. A. Soumeh
Affiliation:
Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark
B. B. Jensen
Affiliation:
Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark
B. Nielsen
Affiliation:
Chr. Hansen A/S, Bøge Allé 10-12, DK-2970 Hørsholm, Denmark
P. Derkx
Affiliation:
Chr. Hansen A/S, Bøge Allé 10-12, DK-2970 Hørsholm, Denmark
M. D. Cantor
Affiliation:
Chr. Hansen A/S, Bøge Allé 10-12, DK-2970 Hørsholm, Denmark
K. Blaabjerg
Affiliation:
Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark
H. D. Poulsen
Affiliation:
Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark
*
†E-mail: janvnoergaard@anis.au.dk
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Abstract

Mutants of Bacillus subtilis can be developed to overproduce Val in vitro. It was hypothesized that addition of Bacillus subtilis mutants to pig diets can be a strategy to supply the animal with Val. The objective was to investigate the effect of Bacillus subtilis mutants on growth performance and blood amino acid (AA) concentrations when fed to piglets. Experiment 1 included 18 pigs (15.0±1.1 kg) fed one of three diets containing either 0.63 or 0.69 standardized ileal digestible (SID) Val : Lys, or 0.63 SID Val : Lys supplemented with a Bacillus subtilis mutant (mutant 1). Blood samples were obtained 0.5 h before feeding and at 1, 2, 3, 4, 5 and 6 h after feeding and analyzed for AAs. In Experiment 2, 80 piglets (9.1±1.1 kg) were fed one of four diets containing 0.63 or 0.67 SID Val : Lys, or 0.63 SID Val : Lys supplemented with another Bacillus subtilis mutant (mutant 2) or its parent wild type. Average daily feed intake, daily weight gain and feed conversion ratio were measured on days 7, 14 and 21. On day 17, blood samples were taken and analyzed for AAs. On days 24 to 26, six pigs from each dietary treatment were fitted with a permanent jugular vein catheter, and blood samples were taken for AA analysis 0.5 h before feeding and at 1, 2, 3, 4, 5 and 6 h after feeding. In experiment 1, Bacillus subtilis mutant 1 tended (P<0.10) to increase the plasma levels of Val at 2 and 3 h post-feeding, but this was not confirmed in Experiment 2. In Experiment 2, Bacillus subtilis mutant 2 and the wild type did not result in a growth performance different from the negative and positive controls. In conclusion, results obtained with the mutant strains of Bacillus subtilis were not better than results obtained with the wild-type strain, and for both strains, the results were not different than the negative control.

Keywords

amino acidsBacillus subtilispigsvaline
Type
Research Article
Information
animal , Volume 10 , Issue 11 , November 2016 , pp. 1796 - 1802
Copyright
© The Animal Consortium 2016 

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