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Evaluation of treatments with hCG and carprofen at embryo transfer in a demi-embryo and recipient virgin heifer model

Published online by Cambridge University Press:  14 March 2013

A. Torres ,
J. Chagas e Silva ,
P. Diniz  and
L. Lopes-da-Costa
A. Torres
Affiliation:
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Avenida da Universidade Técnica, Alto da Ajuda, 1300-477 Lisboa, Portugal
J. Chagas e Silva
Affiliation:
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Avenida da Universidade Técnica, Alto da Ajuda, 1300-477 Lisboa, Portugal
P. Diniz
Affiliation:
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Avenida da Universidade Técnica, Alto da Ajuda, 1300-477 Lisboa, Portugal
L. Lopes-da-Costa*
Affiliation:
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Avenida da Universidade Técnica, Alto da Ajuda, 1300-477 Lisboa, Portugal
*
†E-mail: lcosta@fmv.utl.pt
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Abstract

An in vivo model, combining a low developmental competence embryo (demi-embryo) and a high-fertility recipient (virgin dairy heifer) was used to evaluate the effects of treatment with human chorionic gonadotropin (hCG) and carprofen at embryo transfer (ET) on plasma progesterone (P4) concentrations of recipients and on embryonic growth and survival. Embryos were bisected and each demi-embryo was transferred to a recipient on Day 7 of the estrous cycle. At ET, heifers (n = 163) were randomly allocated to treatment with hCG (2500 IU im), carprofen (500 mg iv), hCG plus carprofen or to untreated controls. Plasma P4 concentrations were measured on Days 0, 7, 14 and 21 of all recipients plus on Days 28, 42 and 63 of pregnant recipients. Pregnancy was presumed to be present in recipients with luteal plasma P4 concentrations until Day 21 and confirmed by using transrectal ultrasonography on Days 28, 42 and 63. Embryonic measurements (crown–rump length and width) were obtained on Day 42. Treatment with hCG induced formation of secondary corpora lutea (CL) in 97% of heifers and increased (P < 0.01) mean plasma P4 concentrations of non-pregnant recipients on Day 14 and of pregnant heifers on Days 14 to 63. This was associated to a significant decrease in early embryonic mortality. In contrast, subsequent embryonic losses resulted in a non-significant numerical increase by 8% of pregnancies maintained to Day 63. Therefore, treatment with hCG significantly rescued embryos through the maternal recognition of pregnancy window but was not able to support development thereafter. Treatment with carprofen at ET had no significant effects on plasma P4 concentrations and rate of embryo mortality. Treatment with hCG plus carprofen at ET induced formation of secondary CL in 90% of heifers but decreased the luteotrophic effect of hCG, resulting in no effect on embryo survival. Low developmental competence embryos showed an intrinsic deficiency in overcoming the maternal recognition of pregnancy challenge and in proceeding to further development until Day 28 of pregnancy, whereas mortality beyond this point was residual. Results on pregnancy rates should be confirmed in further experiments involving a larger sample size.

Keywords

demi-embryohCGcarprofenprogesteronebovine
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 8 , August 2013 , pp. 1317 - 1322
Copyright
Copyright © The Animal Consortium 2013 

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References

Bazer, FW, Wu, G, Spencer, TE, Johnson, GA, Burghardt, RC, Bayless, K 2010. Novel pathways for implantation and establishment and maintenance of pregnancy in mammals. Molecular Human Reproduction 16, 135–152.Google Scholar
Braun, M, Kietzmann, M 2004. Ischaemia-reperfusion injury in the isolated haemoperfused bovine uterus – a model for the investigation of anti-inflammatory substances? ALTEX – Alternatives to Animal Experimentation 21, 49–56.Google Scholar
Brentnall, C, Cheng, Z, McKellar, QA, Lees, P 2012. Potency and selectivity of carprofen enantiomers for inhibition of bovine cyclooxygenase in whole blood assays. Research in Veterinary Science 93, 1387–1392.Google Scholar
Chagas e Silva, J, Lopes-da-Costa, L 2005. Luteotrophic influence of early bovine embryos and the relationship between plasma progesterone concentrations and embryo survival. Theriogenology 64, 49–60.Google Scholar
Chagas e Silva, J, Lopes-da-Costa, L, Robalo Silva, J 2002. Plasma progesterone profiles and factors affecting embryo-fetal mortality following embryo transfer in dairy cattle. Theriogenology 58, 51–59.Google Scholar
Chagas e Silva, J, Cidadão, R, Robalo Silva, J, Lopes-da-Costa, L 1999. Effect of embryo and recipient on pregnancy rate and embryo-fetal mortality following transfer in cattle. Proceedings of 3rd Conference of the European Society for Domestic Animal Reproduction (ESDAR), 26–27 November 1999, Angers, France, pp. 47–48.Google Scholar
Clemente, M, de La Fuente, J, Fair, T, Al Naib, A, Gutierrez-Adan, A, Roche, JF, Rizos, D, Lonergan, P 2009. Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium? Reproduction 138, 507–517.CrossRefGoogle ScholarPubMed
Cooperative Regional Research Project, NE.-161 1996. Relationship of fertility patterns of follicular development and associated hormonal profiles in dairy cows and heifers. Journal of Animal Science 74, 1943–1952.Google Scholar
De Rensis, F, López-Gatius, F, García-Ispierto, I, Techakumpu, M 2010. Clinical use of human chorionic gonadotropin in dairy cows: an update. Theriogenology 73, 1001–1008.Google Scholar
Elli, M, Gaffuri, B, Frigerio, A, Zanardelli, M, Covini, D, Candiani, M, Vignali, M 2001. Effect of a single dose of ibuprofen lysinate before embryo transfer on pregnancy rates in cows. Reproduction 121, 151–154.Google Scholar
Garrett, JE, Geisert, RD, Zavy, MT, Morgan, GL 1988. Evidence for maternal regulation of early conceptus growth and development in beef cattle. Journal of Reproduction and Fertility 84, 437–446.Google Scholar
Gaytán, M, Morales, C, Bellido, C, Sánchez-Criado, JE, Gaytán, F 2006. Non-steroidal anti-inflammatory drugs (NSAIDs) and ovulation: lessons from morphology. Histology and Histopathology 21, 541–556.Google Scholar
Geary, TW, Ansotegui, RP, MacNeil, MD, Roberts, AJ, Waterman, RC 2010. Effects of flunixin meglumine on pregnancy establishment in beef cattle. Journal of Animal Science 88, 943–949.CrossRefGoogle ScholarPubMed
Guzeloglu, A, Erdem, H, Saribay, MK, Thatcher, WW, Tekeli, T 2007. Effect of the administration of flunixin meglumine on pregnancy rates in Holstein heifers. Veterinary Record 160, 404–406.CrossRefGoogle ScholarPubMed
Heuwieser, W, Iwersen, M, Goetze, L 2011. Efficacy of carprofen on conception rates in lactating dairy cows after subcutaneous or intrauterine administration at the time of breeding. Journal of Dairy Science 94, 146–151.Google Scholar
Humblot, P 2001. Use of pregnancy specific proteins and progesterone assays to monitor pregnancy and determine the timing, frequencies and sources of embryonic mortality in ruminants. Theriogenology 56, 1417–1433.CrossRefGoogle ScholarPubMed
Kerbler, TL, Buhr, MM, Jordan, LT, Leslie, KE, Walton, JS 1997. Relationship between maternal plasma progesterone concentration and interferon-tau synthesis by the conceptus in cattle. Theriogenology 47, 703–714.CrossRefGoogle ScholarPubMed
Lonergan, P 2011. Influence of progesterone on oocyte quality and embryo development in cows. Theriogenology 76, 1594–1601.Google Scholar
Lopes-da-Costa, L, Chagas e Silva, J, Deloche, MC, Jeanguyot, N, Humblot, P, Horta, AEM 2011. Effects of embryo size at transfer (whole versus demi) and early pregnancy progesterone supplementation on embryo growth and pregnancy-specific protein bovine concentrations in recipient dairy heifers. Theriogenology 76, 522–531.Google Scholar
Ludwig, B, Jordan, JC, Rehm, WF, Thun, R 1989. Carprofen in veterinary medicine. I. Plasma disposition, milk excretion and tolerance in milk-producing cows. Schweiz Arch Tierheilkd 131, 99–106.Google ScholarPubMed
Mann, GE, Lamming, GE 2001. Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction 121, 175–180.Google Scholar
Mann, GE, Lamming, GE, Payne, JH 1998. Role of early luteal phase progesterone in control of the timing of the luteolytic signal in cows. Journal of Reproduction and Fertility 113, 47–51.Google Scholar
Merril, ML, Ansotegui, RP, Burns, PD, MacNeil, MD, Geary, TW 2007. Effects of flunixin meglumine and transportation stress on pregnancy establishment in beef cows. Journal of Animal Science 85, 1547–1554.Google Scholar
Nishigai, M, Kamomae, H, Tanaka, T, Kaneda, Y 2002. Improvement of pregnancy rate in Japanese Black cows by administration of hCG to recipients of transferred frozen-thawed embryos. Theriogenology 58, 1597–1606.Google Scholar
Pretheeban, T, Balendran, A, Gordon, MB, Rajamahendran, R 2010. mRNA of luteal genes associated with progesterone synthesis, maintenance, and apoptosis in dairy heifers and lactating dairy cows. Animal Reproduction Science 121, 218–224.Google Scholar
Purcell, SH, Beal, WE, Gray, KR 2005. Effect of a CIDR insert and flunixin meglumine, administered at the time of embryo transfer, on pregnancy rate and resynchronization of estrus in beef cattle. Theriogenology 64, 867–878.Google Scholar
Rabaglino, MB, Risco, CA, Thatcher, M-J, Lima, F, Santos, JEP, Thatcher, WW 2010. Use of a five-day progesterone-based timed AI protocol to determine if flunixin meglumine improves pregnancy per timed AI in dairy heifers. Theriogenology 73, 1311–1318.CrossRefGoogle ScholarPubMed
Sartori, R, Sartor-Bergfelt, R, Mertens, SA, Guenther, JN, Parrish, JJ, Wiltbank, MC 2002. Fertilization and early embryonic development in heifers and lactating cows in summer and lactating and dry cows in winter. Journal of Dairy Science 85, 2803–2812.CrossRefGoogle ScholarPubMed
Scenna, FN, Hockett, ME, Towns, TM, Saxton, AM, Rohrbach, NR, Wehrman, ME, Schrick, FN 2005. Influence of a prostaglandin synthesis inhibitor administered at embryo transfer on pregnancy rates of recipient cows. Prostaglandins & Other Lipid Mediators 78, 38–45.Google Scholar
Schrick, FN, Inskeep, EK, Butcher, RL 1993. Pregnancy rates for embryos transferred from early postpartum beef cows into recipients with normal estrous cycles. Biology of Reproduction 49, 617–621.Google Scholar
Shelton, K, Gayerie De Abreu, MF, Hunter, MG, Parkinson, TJ, Lamming, GE 1990. Luteal inadequacy during the early luteal phase of subfertile cows. Journal of Reproduction and Fertility 90, 1–10.Google Scholar
Sianangama, PC, Rajamahendran, R 1992. Effect of human chorionic gonadotropin administered at specific times following breeding on milk progesterone and pregnancy in cows. Theriogenology 38, 85–96.Google Scholar
Stevenson, JS, Tiffany, SM, Inskeep, EK 2008. Maintenance of pregnancy in dairy cattle after treatment with human chorionic gonadotropin or gonadotropin-releasing hormone. Journal of Dairy Science 91, 3092–3101.Google Scholar
Stringfellow, DA, Seidel, SM (ed.) 1998. Manual of the international embryo transfer society. International Embryo Transfer Society Inc., Savoy, IL, USA.Google Scholar
von Krueger, X, Heuwieser, W 2010. Effect of flunixin meglumine and carprofen on pregnancy rates in dairy cattle. Journal of Dairy Science 93, 5140–5146.Google Scholar
Wallace, LD, Breiner, CA, Breiner, RA, Spell, AR, Carter, JA, Lamb, GC, Stevenson, JS 2011. Administration of human chorionic gonadotropin at embryo transfer induced ovulation of a first wave dominant follicle, and increased progesterone and transfer pregnancy rates. Theriogenology 75, 1506–1515.Google Scholar
Wiltbank, MC, Souza, AH, Carvalho, PD, Bender, RW, Nascimento, AB 2011. Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle. Reproduction, Fertility and Development 24, 238–243.Google Scholar