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Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relationship with productivity of rabbit does

Published online by Cambridge University Press:  15 April 2008

M. Theau-Clément ,
F. Lebas ,
J. F. Beckers  and
P. V. Drion
M. Theau-Clément*
Affiliation:
Institut National de la Recherche Agronomique, Station d’Amélioration Génétique des Animaux, BP 52627 – 31326 Castanet-Tolosan Cedex, France
F. Lebas
Affiliation:
Cuniculture, 87a Chemin de Lasserre, 31450 Corronsac, France
J. F. Beckers
Affiliation:
Université de Liège, Physiologie de la Reproduction, Faculté de Médecine Vétérinaire, Bd de Colonster N°20, B41, P71B-400, Sart-Tilman, Belgium
P. V. Drion
Affiliation:
Université de Liège, Physiologie de la Reproduction, Faculté de Médecine Vétérinaire, Bd de Colonster N°20, B41, P71B-400, Sart-Tilman, Belgium
*
E-mail: Michele.Theau-Clement@toulouse.inra.fr
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Abstract

The aim of this experiment was to study the kinetics of anti-eCG (equine chorionic gonadotrophin) antibodies in relation to eCG dose (8 or 25 IU) and number of injections (n = 11) in comparison with a control group (no injection), and to relate antibody production to sexual receptivity and productivity of rabbit does. In all, 124 lactating primiparous rabbit does were inseminated every 35 days for a year. Just before eCG injection (48 h before insemination), blood samples were collected from all the does to assay anti-eCG antibodies. The anti-eCG antibody binding rate, regardless of the injected dose, shows that none of the does developed detectable anti-eCG antibodies before the 7th injection. The level of detectable anti-eCG antibodies began to show an increase at the 7th injection and was significant only for the 25 IU dose at the 11th injection. At the end of the experiment, 15% and 39% of does treated with 8 and 25 IU, respectively, developed immunity to eCG (binding rate >6%: higher binding rate of the control group). Consequently, the immune response depends on the eCG dose and on the number of injections. Moreover, productivity of does estimated from the number of weaned rabbits produced per insemination is not influenced by the level of eCG antibodies (7.0 and 6.9 for binding rate <6% and binding rate ⩾6%, respectively). Only 19 inseminations (n = 6 and n = 13 for 8 and 25 IU, respectively) were made on hyperimmune does. Consequently, the immune response to eCG seems to be marginal for rabbit does. Moreover, under the described experimental conditions, reproductive performances of hyperimmune does were not affected.

Keywords

rabbiteCGanti-eCG antibodiesimmune responseproductivity
Type
Full Paper
Information
animal , Volume 2 , Issue 5 , May 2008 , pp. 746 - 751
Copyright
Copyright © The Animal Consortium 2008

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References

Baril, G, Remy, B, Vallet, JC, Beckers, JF 1992. Effect of repeated use of progestagen-PMSG treatment for oestrus control in dairy goats out of breeding season. Reproduction in Domestic Animals 27, 161–168.CrossRefGoogle Scholar
Bodin L, Brice G, Remy B, Maurel MC and Beckers JF 1995. Effects of repeated PMSG treatments on sheep reproduction. In Proceedings of the 46th Annual Meeting of the EAAP, 1995, Prague, Czech Republic.Google Scholar
Boiti, C, Castellini, C, Canali, C, Zampini, D, Monaci, M 1995. Long term effect of PMSG on rabbit does reproductive performance. World Rabbit Science 3, 51–56.Google Scholar
Bourdillon, A, Chmitelin, F, Jarrin, D, Parez, V, Rouiller, H 1992. Effect of PMSG treatment on breeding result of artificial inseminated rabbits. Journal of Applied Rabbit Research 15, 530–537.Google Scholar
Canali C, Boiti C, Castellini C and Zampini D 1991. Riposta anticorpale delle coniglie trattate ripetutamente con PMSG nella pratica della sincronizzazione degli estri. 2° Meeting Nazionale Studio della Efficienza Riproduttiva degli Animali di Interesse Zootecnico, Bergamo, Italie 24 Novembre, 1989, 103–108.Google Scholar
Clarke, IJ 1973. Repeated gonadotrophic stimulation of ewes. Proceedings of New Zealand Society of Animal Production 33, 204.Google Scholar
Drion, PV, Remy, B, Houtain, JY, Mc Namara, M, Baril, G, Heyman, Y, Cognie, Y, Theau-Clément, M, Leboeuf, B, Ectors, F, Segers, K, Beckers, JF 1998. Utilisation répétée des gonadotropines exogènes dans le contrôle de la reproduction : justifications, relations structure-activité biologique, effets secondaires potentiels. Une synthèse. Annales de Médecine Vétérinaire 142, 373–396.Google Scholar
International Rabbit Reproduction Group 2005. Recommendations and guidelines for applied reproduction trials with rabbit does. World Rabbit Science 13, 147–164.Google Scholar
Lebas, F, Theau-Clément, M, Remy, B, Drion, P, Beckers, JF 1996. Production of anti-PMSG antibodies and its relation to the productivity of rabbit does. World Rabbit Science 4, 57–62.Google Scholar
Rebollar, PG, Milanès, A, Pereda, N, Millán, P, Cano, P, Esquifino, AI, Villarroel, M, Silván, G, Lorenzo, PL 2006. Oestrus synchronisation of rabbit does at early post-partum by doe-litter separation or ECG injection: reproductive parameters and endocrine profiles. Animal Reproduction Science 93, 218–230.Google Scholar
Roy, F, Maurel, MC, Combarnous, Y, Briois, JP, Pobel, T, Deletang, F 1995. Etude de la réponse immunitaire observée chez les ovins et les caprins traités avec PMSG dans le cadre de l’insémination artificielle. Rencontres Recherches Ruminants 3, 395–398.Google Scholar
Roy, F, Maurel, MC, Combes, B, Vaiman, D, Cribiu, EP, Lantier, I, Pobel, T, Delétang, F, Combarnous, Y, Guillou, F 1999. The negative effect of repeated equine chorionic gonadotropin treatment on subsequent fertility in Alpine goats is due to a humoral immune response involving the Major Histocompatibility Complex. Biology of reproduction 60, 805–813.CrossRefGoogle ScholarPubMed
SAS (Statistical Analysis Systems Institute) 1993. Release 6.09 SAS Technical Report P-243.SAS/STAT® Software. Cary, NC, USA.Google Scholar
Stradaioli G, Castellini C, Monaci M, Canali C, Verini Supplizi A and Boiti C 1994. Effetti del PMSG sulle prestazioni riproduttive delle coniglie: risultati di un quadriennio di sperimentazione. 6° Meeting Nazionale Studio della Efficienza Riproduttiva degli Animali di Interesse Zootecnico, 29 Aprile, 1994, Bergamo, Italie, 153–157.Google Scholar
Theau-Clément M, Lebas F and Mercier P 2007. Influence of different eCG doses on sexual receptivity and productivity of rabbit does. World Rabbit Science, in press.Google Scholar
Thorell, JI, Johansson, BG 1971. Enzymatic iodination of polypeptides with 125I to high specific activity. Biochemica and Biophysica Acta 251, 363–369.CrossRefGoogle ScholarPubMed